Studies on bilirubin metabolism in fish

1. 对黄疸黄尾鱼各组织中TBA值、α - toc含量进行了分析。采用高效液相色谱法和环评法对黄疸黄尾鱼血浆中胆红素含量进行了分析。黄疸鱼组织中脂质过氧化物进展，血浆中胆红素X增高。这些结果证实活性氧可能是黄疸的主要原因。研究黄尾鱼分别饲喂3种不同脂质过氧化水平（I-111组）和9 %沙丁鱼油(POV分别为4.7 （IV组）和100 （V组）的褐鱼粉饲料饲喂30 d，观察黄尾鱼肝脏中a-生育酚（α -toc）含量、2-硫代巴比托酸活性物质（TBARS）值以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性的变化。饲料中脂质过氧化素含量最高或含有脂质过氧化物的饲料可使黄尾鱼肝脏发生体内脂质过氧化，抑制体内脂质过氧化可预防黄尾鱼黄疸。1989年4月和6月在渔场捕获的红鲷鱼中观察到高胆红素血症。原因可能与生殖有关。分析了黄尾鱼体内脂质过氧化和自由基清除能力的季节变化。结果说明了黄疸主要发生在夏季的原因。制备并鉴定了非偶联胆红素lx α特异性抗胆红素lx α单克隆抗体。抗体仅与胆红素ix α反应，而不与偶联胆红素lx α反应。采用重氮法和采用抗胆红素- lx α单克隆抗体的EIA法分析几种疾病患者血清中胆红素的含量。并对胆红素X的结构进行了研究。结果表明，胆红素是最原始、最有效的自由基清除剂。

1. TBA values, content of alpha-Toc in some tissues of jaundiced yellowtail were analyzed. Bilirubin contents in the plasma of jaundiced yellowtail were analyzed by HPLC and EIA method. In vivh lipid peroxidadon progressed in the tissues of jaundiced fish and so call blirubin X increased in the plasma. These results confirm that the active oxygen species may be a piincipale cause of the jaundice.2. Yellowtail were fed on three brown fish meal diets whose lipid peroxide levels were deffemt (group I-111) or brown fishmeal 9 % of sardine oil whose POV was 4.7 (group IV) or 100 (group V) for 30 days and changes in a-tocopherol (alpha-Toc) contents, 2-thiobarbituric acid reactive substances (TBARS) values and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the liver were analyzed. In vivo lipid peroxidaion may progress in the liver of yellowtail fed on diets whose lipid peroxidatin was the highest levels or which contain lipid peroxides and that the suppression of in vivo lipid peroxidation may prevent the jaundice of yellowtail.3. Highperbilirubinemia were observed in red sea bream obtained from fish farm in April and June, 1989. The cause may probably related to the reproduction.4. Seasonal changes of in vivo lipid peroxidation and free radical scavenging abilty of yellowtail were analyzed. Results obtained account for the reason that the jaundice of yellowtail occurred mainly in summer.5. Anti-bilirubin-lX alpha monoclonal antibodies exclusively specific for unconjugated bilirubin-lX alpha are prepared and characterized. The antibodies reacted only bilirubin-IX alpha but did not react with conjugated bilirubin-lX alpha.6. Bilirubin contents in the serum of several disease were analyzed by diazo method and EIA method in which antibilirubin-lX alpha monoclonal antibodies were used. Structure of so call bilirubin X were also studied. Results obtained indicate that bilirubin is the most primitive and effective free radical scavenger.