Studies on bilirubin metabolism in fish

1.分析了黄尾鱼部分组织中TBA值和α-Toc含量。采用高效液相色谱法和酶免疫分析法测定了黄疸黄尾鱼血浆中胆红素的含量。在体内，黄疸鱼的组织中脂质过氧化作用进行，因此在血浆中称之为胆红素X增加。这些结果证实了活性氧可能是黄疸的主要原因.用三种过氧化脂质水平不同的褐鱼粉饲料饲养黄尾鱼，（I-111组）或棕鱼粉9%的沙丁鱼油，其溶解度为4.7（IV组）或100（V组）30天，以及α-生育酚的变化（α-Toc）含量，分析肝脏中2-硫代巴比妥酸反应物质（TBARS）值和超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性。黄尾鱼肝脏在体内可能发生脂质过氧化反应，而在体内抑制脂质过氧化反应可以预防黄尾鱼的黄疸. 1989年4月和6月，从养殖场采集的真鲷出现高胆红素血症。其原因可能与繁殖有关.分析了黄尾鱼体内脂质过氧化和清除自由基能力的季节性变化。以上结果解释了黄尾鱼黄疸主要发生在夏季的原因.制备并表征了对未缀合的胆红素-IX α特异性的抗胆红素-IX α单克隆抗体。这些抗体仅与珠蛋白-IX α反应，但不与缀合的珠蛋白-IX α 6反应。用重氮法和EIA法测定了几种疾病血清中胆红素的含量。对胆红素X的结构进行了研究。结果表明，胆红素是最原始、最有效的自由基清除剂。

1. TBA values, content of alpha-Toc in some tissues of jaundiced yellowtail were analyzed. Bilirubin contents in the plasma of jaundiced yellowtail were analyzed by HPLC and EIA method. In vivh lipid peroxidadon progressed in the tissues of jaundiced fish and so call blirubin X increased in the plasma. These results confirm that the active oxygen species may be a piincipale cause of the jaundice.2. Yellowtail were fed on three brown fish meal diets whose lipid peroxide levels were deffemt (group I-111) or brown fishmeal 9 % of sardine oil whose POV was 4.7 (group IV) or 100 (group V) for 30 days and changes in a-tocopherol (alpha-Toc) contents, 2-thiobarbituric acid reactive substances (TBARS) values and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in the liver were analyzed. In vivo lipid peroxidaion may progress in the liver of yellowtail fed on diets whose lipid peroxidatin was the highest levels or which contain lipid peroxides and that the suppression of in vivo lipid peroxidation may prevent the jaundice of yellowtail.3. Highperbilirubinemia were observed in red sea bream obtained from fish farm in April and June, 1989. The cause may probably related to the reproduction.4. Seasonal changes of in vivo lipid peroxidation and free radical scavenging abilty of yellowtail were analyzed. Results obtained account for the reason that the jaundice of yellowtail occurred mainly in summer.5. Anti-bilirubin-lX alpha monoclonal antibodies exclusively specific for unconjugated bilirubin-lX alpha are prepared and characterized. The antibodies reacted only bilirubin-IX alpha but did not react with conjugated bilirubin-lX alpha.6. Bilirubin contents in the serum of several disease were analyzed by diazo method and EIA method in which antibilirubin-lX alpha monoclonal antibodies were used. Structure of so call bilirubin X were also studied. Results obtained indicate that bilirubin is the most primitive and effective free radical scavenger.