Studies on Bilirubin Excretion Mechanism of Fish

1.建立了鱼肉中胆色素的高效液相色谱分析方法。该方法快速、灵敏、可靠，可用于鱼类胆汁和其他组织中胆汁色素的测定。用高效液相色谱法分析了牛磺酸与苯肼所致的溶血性贫血黄鱼胆汁中胆汁色素含量的变化。结果表明，牛磺酸在黄鱼胆红素排泄机制中起重要作用。对黄尾鱼的TBA值、α-生育酚和胆汁色素含量进行了分析。肝、脾组织学观察。结果证实，肝脏中脂肪酸的过氧化反应参与了黄疸的发生。通过对游离酸及其邻氨基苯甲酸乙酯重氮衍生物的高效液相色谱分析，在鳗鱼胆汁中检测到胆红素-IXβ异构体。胆红素-9β占胆红素总量的30-40%。对黄褐鳗肝脏进行了组织病理学和酶免疫组织化学观察。用高效液相色谱法测定血浆胆红素含量。结果证实内分泌系统功能障碍是该病发生的原因之一。

1. HPLC method for analysis of bile pigment in fish was constructed. The method is rapid, sensitive, and reliable and seems to be available for measurement of bile pigments in the bile and other tissues of fish.2. Changes in bile pigment contents in the bile of yellowtail fed on taurine with phenylhydrazine induced hemolytic anemia were analyzed by HPLC. The results indicate that taurine plays an important role for bilirubin excretion mechanism of yellowtail.3. TBA values, content of alpha-tocopherol and bile pigments in jaundiced yellowtail were analyzed. The liver and spleen were also observed histologically. The results confirm that the peroxidation of fatty acids in the liver contributes the occurrence of the jaundice.4. Bilirubin-IX beta isomer was detected in the bile of eel, as evidenced by HPLC analysis of free acid and its ethyl anthranilate diazo derivatives. Bilirubin-IX beta accounted for 30-40 % of the total bilirubins in the bile.5. The liver of jaundiced eel was observed histopathologically and enzyme-imunochemically. Bilirubin contents in the plasma were also analyzed by HPLC. The results confirm that the dysfunction of the endocrine systems contributes the occurrence of the disease.