Studies on antioxidant activities of bilirubin and in vivo lipid peroxidation of fish.

1.用膨化饲料（EP）和未注入细菌（JB）喂养五角黄尾鱼2个月，细菌感染被认为是导致黄疸的原因之一。注射引起溶血。溶血引起体内脂质过氧化，消耗α -生育酚（α -toc）和抗坏血酸（ASA）来防止体内脂质过氧化。在不同POV、α - toc和ASA水平的沙丁鱼油中饲喂黄尾鱼2个月，高α - toc和ASA水平饲料的黄尾鱼的苯基氢氮和jb .2-硫代巴比妥酸活性物质（TBARS）值和磷脂过氧化氢（PH）含量显著低于低α - toc和ASA水平饲料的黄尾鱼。饲粮中α - toc和ASA含量高的鱼血浆胆红素水平明显低于饲粮中α - toc和ASA含量低的鱼。建立了柱后衍生高效液相色谱法同时测定鱼类组织中抗坏血酸（AA）和脱氢抗坏血酸（DHA）的方法。提取的AA和DHA用Shim-pack SCR-101H色谱柱在20 min内分离，与含硼氢化钠的氢氧化钠反应，300nm下监测。AA和DHA的检出限均为0.1 μ g/ml。分别饲喂高水平的L-抗坏血酸1-2-磷酸Mg （APM）和ASA的EP （H组）和低水平的APM和ASA （L组）2个月。H组血浆和肝脏TBARS值及PH含量均低于L组，H组肝脏ASA含量高于L组，但血浆ASA水平与L组相近。注射JB后，两组血浆胆红素水平相近。提示血浆ASA水平不足以预防H.5组黄疸的发生。用红细胞包涵体综合征（EIBS）病毒注射银鲑。血浆胆红素水平略有升高。从肝脏TBARS值和红细胞膜脂肪酸组成判断，鱼体内发生了脂质过氧化。少

1.Yellowtail Seriola quinqueradiata were fed on extruder diets (EP) for 2 month and unjected bacteria (JB) whose infection is thought to one of the cause of jaudice. The injection induced to hemolysis. Hemolysis occurred bring about in vivo lipid peroxidation and alpha-tocopherol (alpha-Toc) and ascorbic acid (ASA) were consumed to prevent in vivo lipid peroxidation.2.Yellowtail were fed on EP containing different POV,alpha-Toc and ASA levels of sardine oil for 2 months and the phenyl hydorazine and JB.2-Thiobarbituric acid reactive substances (TBARS) values and phospholipids hydroperoxides (PH) contents of fish fed on diet containing high levels of alpha-Toc and ASA were much lower than those of fish fed on EP containing low level of alpha-Toc and ASA.Plasma bilirubin level of fish fed on diet containing high level of alpha-Toc and ASA were much lower than those of fish fed on diet containing low of alpha-Toc and ASA.3.Simultaneous determination of ascorbic acid (AA) and dehydroascorb … More ic acid (DHA) in fish tissues has been developed by high performance liquid chromatography with postcolumn derivatization method. AA and DHA extracted were separated by a Shim-pack SCR-101H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300nm. The detection limits for both AA and DHA were 0.1 mug/ml.4.Yellowtail were fed on EP containing high level of L-Ascorby1-2-phosphate Mg (APM) and ASA for 2 months (group H) or low level of APM and ASA (group L). The plasma and liver TBARS value and PH contents were lower in group H than in group L.Liver ASA contents in group H was higher than those in group L but plasma ASA levels were similar to both qroups When JB was injected to the fish plasma bilirubin levels, plasma bilirubin levels were similar to both groups. These results suggest that plasma ASA level was not enough to prevent jaundice of group H.5.Coho salmon was injected with erythrocytic inclusion body syndrome (EIBS) virus. Plasma bilirubin levels were slightly increased. Judging from the liver TBARS values and fatty acid composition of erythrocytes membrane, in vivo lipid peroxidation was occurred in the fish. Less