Studies on antioxidant activities of bilirubin and in vivo lipid peroxidation of fish.

鱼类胆红素抗氧化活性及体内脂质过氧化作用的研究。

• 批准号: 07660272
• 负责人: SAKAI Tadashi
• 金额: $ 1.41万
• 依托单位: Miyazaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660272/
• 关键词: Bilirubin; Jaundice; Yellowtail; Substance X; alpha-Tocopherol; Ascorbic Acid; Extruder Treatment; Fish Meal

1.Yellowtail Seriola quinqueradiata were fed on extruder diets (EP) for 2 month and unjected bacteria (JB) whose infection is thought to one of the cause of jaudice. The injection induced to hemolysis. Hemolysis occurred bring about in vivo lipid peroxidation and alpha-tocopherol (alpha-Toc) and ascorbic acid (ASA) were consumed to prevent in vivo lipid peroxidation.2.Yellowtail were fed on EP containing different POV,alpha-Toc and ASA levels of sardine oil for 2 months and the phenyl hydorazine and JB.2-Thiobarbituric acid reactive substances (TBARS) values and phospholipids hydroperoxides (PH) contents of fish fed on diet containing high levels of alpha-Toc and ASA were much lower than those of fish fed on EP containing low level of alpha-Toc and ASA.Plasma bilirubin level of fish fed on diet containing high level of alpha-Toc and ASA were much lower than those of fish fed on diet containing low of alpha-Toc and ASA.3.Simultaneous determination of ascorbic acid (AA) and dehydroascorb … More ic acid (DHA) in fish tissues has been developed by high performance liquid chromatography with postcolumn derivatization method. AA and DHA extracted were separated by a Shim-pack SCR-101H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300nm. The detection limits for both AA and DHA were 0.1 mug/ml.4.Yellowtail were fed on EP containing high level of L-Ascorby1-2-phosphate Mg (APM) and ASA for 2 months (group H) or low level of APM and ASA (group L). The plasma and liver TBARS value and PH contents were lower in group H than in group L.Liver ASA contents in group H was higher than those in group L but plasma ASA levels were similar to both qroups When JB was injected to the fish plasma bilirubin levels, plasma bilirubin levels were similar to both groups. These results suggest that plasma ASA level was not enough to prevent jaundice of group H.5.Coho salmon was injected with erythrocytic inclusion body syndrome (EIBS) virus. Plasma bilirubin levels were slightly increased. Judging from the liver TBARS values and fatty acid composition of erythrocytes membrane, in vivo lipid peroxidation was occurred in the fish. Less

1.用挤压膨化饲料（EP）和未注入的细菌（JB）饲养黄尾马齿螺（Seriola quinqueradiata）2个月，JB的感染被认为是引起黄尾病的原因之一。注射导致溶血。发生溶血，导致体内脂质过氧化和α-生育酚（α-Toc）和抗坏血酸（阿萨）来防止体内脂质过氧化。沙丁鱼油2个月的alpha-Toc和阿萨水平以及苯基海拉嗪和JB. 2-硫代巴比妥酸反应物质（TBARS）值和磷脂氢过氧化物（PH）高α-Toc和阿萨组的血浆胆红素水平显著低于低α-Toc和ASA组，高α-Toc和ASA组的血浆胆红素水平显著低于低α-Toc和ASA组，高α-Toc和阿萨组的血浆胆红素水平显著低于低α-Toc和ASA组。抗坏血酸和脱氢抗坏血酸的同时测定 ...更多信息 采用高效液相色谱柱后衍生法测定了鱼组织中的DHA。提取的AA和DHA经Shim-pack SCR-101 H色谱柱在20 min内分离，与含硼氢化钠的氢氧化钠反应，在300 nm处监测。AA和DHA的检测限均为0.1 μ g/ml。4.黄尾鱼饲喂高水平L-抗坏血酸镁（APM）和阿萨的EP（H组）或低水平APM和阿萨的EP（L组）2个月。血浆和肝脏TBARS值及PH值H组低于L组，肝脏阿萨含量H组高于L组，而血浆阿萨水平H组与L组相近。结果表明，血浆阿萨水平不足以预防H组的黄疸。血浆胆红素水平略微升高。从肝脏TBARS值和红细胞膜脂肪酸组成来看，该鱼体内发生了脂质过氧化反应。少

项目成果

Ito, T., Murata, H., Yasui, Y., Matui, M., Sakai, T.et al.: "Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography." J.Chromatogr.Vol.667. 355-357 (1995)

Ito, T.、Murata, H.、Yasui, Y.、Matui, M.、Sakai, T.等人：“采用高效液相色谱法同时测定鱼组织中的抗坏血酸和脱氢抗坏血酸。”