Studies on antioxidant activities of bilirubin and in vivo lipid peroxidation of fish.

1.用挤压机饲料(EP)和未注射细菌(JB)饲喂五叶黄尾鱼2个月，其感染被认为是引起黄褐斑的原因之一。注射后引起溶血。溶血导致体内脂质过氧化，消耗α-生育酚(α-Toc)和抗坏血酸(ASA)以防止体内脂质过氧化。饲喂高水平α-ToC和AsA饲料的鱼，其血浆胆红素水平显著低于投喂低水平α-ToC和AsA饲料的鱼；饲喂高水平α-ToC和AsA饲料的鱼，其血浆胆红素水平显著低于投喂低α-ToC和AsA饲料的鱼。3.同时测定抗坏血酸(AA)和脱氢抗坏血酸…的含量采用高效液相色谱-柱后衍生法测定了鱼类组织中较多的脂肪酸(DHA)。提取的AA和DHA用Shim-pack SCR-101H色谱柱在20min内分离，与含硼氢化钠的氢氧化钠反应，在300 nm处检测。黄尾鱼以高水平的L抗坏血酸镁和抗坏血酸饲喂2个月(H组)或低水平抗坏血酸和抗坏血酸镁(L组)饲养2个月。H组血浆和肝组织的TBARS值和PH含量均低于L组。肝脏AsA含量H组高于L组，但血浆AsA水平接近两组。这些结果表明，血浆AsA水平不足以预防H.5组大鼠黄疸的发生。血浆胆红素水平略有升高。从肝脏的TBARS值和红细胞膜的脂肪酸组成来看，该鱼体内发生了脂质过氧化。较少

1.Yellowtail Seriola quinqueradiata were fed on extruder diets (EP) for 2 month and unjected bacteria (JB) whose infection is thought to one of the cause of jaudice. The injection induced to hemolysis. Hemolysis occurred bring about in vivo lipid peroxidation and alpha-tocopherol (alpha-Toc) and ascorbic acid (ASA) were consumed to prevent in vivo lipid peroxidation.2.Yellowtail were fed on EP containing different POV,alpha-Toc and ASA levels of sardine oil for 2 months and the phenyl hydorazine and JB.2-Thiobarbituric acid reactive substances (TBARS) values and phospholipids hydroperoxides (PH) contents of fish fed on diet containing high levels of alpha-Toc and ASA were much lower than those of fish fed on EP containing low level of alpha-Toc and ASA.Plasma bilirubin level of fish fed on diet containing high level of alpha-Toc and ASA were much lower than those of fish fed on diet containing low of alpha-Toc and ASA.3.Simultaneous determination of ascorbic acid (AA) and dehydroascorb … More ic acid (DHA) in fish tissues has been developed by high performance liquid chromatography with postcolumn derivatization method. AA and DHA extracted were separated by a Shim-pack SCR-101H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300nm. The detection limits for both AA and DHA were 0.1 mug/ml.4.Yellowtail were fed on EP containing high level of L-Ascorby1-2-phosphate Mg (APM) and ASA for 2 months (group H) or low level of APM and ASA (group L). The plasma and liver TBARS value and PH contents were lower in group H than in group L.Liver ASA contents in group H was higher than those in group L but plasma ASA levels were similar to both qroups When JB was injected to the fish plasma bilirubin levels, plasma bilirubin levels were similar to both groups. These results suggest that plasma ASA level was not enough to prevent jaundice of group H.5.Coho salmon was injected with erythrocytic inclusion body syndrome (EIBS) virus. Plasma bilirubin levels were slightly increased. Judging from the liver TBARS values and fatty acid composition of erythrocytes membrane, in vivo lipid peroxidation was occurred in the fish. Less