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The expression of Mn-SOD in reproductive and endocrine organs.

The expression of Mn-SOD in reproductive and endocrine organs.
Mn-SOD在生殖和内分泌器官中的表达。
批准号:
05670012
负责人:
SASAKI Junzo
金额:
$0.9万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1993
资助国家:
日本
项目状态:
已结题
起止时间:
1993 至 1994

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中文摘要
翻译
人们一直强调,超氧化物歧化酶(SOD)具有清道夫酶的作用。以前,我们曾报道过锰-超氧化物歧化酶参与类固醇的合成。本研究采用原位杂交技术,检测了不同实验条件下大鼠雌性生殖器官和内分泌器官中Mn-SOD mRNA的表达,获得了以下结果。结论:1.在PMSG/hCG排卵早期,内膜细胞中有明显的Mn-SOD基因表达。在排卵的卵泡上皮细胞中,锰-超氧化物歧化酶的mRNA表达增加,之后在黄体中的表达增加。注射PMSG后,子宫内膜上皮细胞中Mn-SOD mRNA的表达开始增加,6-24 h达高峰,48 h后表达下降。在发情周期中,锰-超氧化物歧化酶的表达水平发生了变化。在含有血红蛋白代谢物的白念珠菌细胞中有明显的表达。雌激素诱导未成熟大鼠子宫内膜锰-超氧化物歧化酶的表达。在肾上腺中,Mn-SOD的表达定位于束状带和网状带,表现出活跃的类固醇激素生成。在去垂体大鼠的肾上腺、卵巢和子宫中,锰-超氧化物歧化酶的表达减少,表明锰-超氧化物歧化酶的表达与细胞代谢包括类固醇激素的合成密切相关。
英文摘要
It has been emphasized that superoxide dismutase (SOD) has a role as a scavenger enzyme. Previously, we reported that Mn-SOD were involved in steroidogenesis. In the present study, we examined the expression of Mn-SOD mRNA, in the female reproductive organs and endocrine organs of the rats under various experimental conditions using in situ hybridization technique and obtained the following results.1. Mn-SOD mRNA was expressed markedly in the theca interna cells during the early PMSG/hCG ovulatory process.2. Mn-SOD mRNA increased in the epithelial cells of the ovulated follicles and later, increased in luteal bodies.3. The expression of Mn-SOD mRNA in epithelial cells of endometrium began to increase by PMSG administration, reached maximum 6-24 h after hCG injection, and decreased 48 h after hCG injection.4. The level of Mn-SOD mRNA expression was shown to change during the estrous cycle. The marked expression was found in the cells of corpus albicans which contained hemoglobin metabolites.5. Estrogen induced the expression of Mn-SOD mRNA in the endometrium of immature rats.6. The expression of Mn-SOD in adrenal gland was localized to zona fasciculata and zona reticularis which showed active steroidogenesis.7. The expression of Mn-SOD mRNA decreased in adrenal gland, ovary and uterus of hypophysecto-mized rats.These results showed that Mn-SOD expression are deeply concerned in cellular metabolism including steroidogenesis.
期刊论文(54)
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会议论文
Watanabe,S et al.: "Selective statng of the superficial cells of mouse urinary bladder epithelium by horseradisdr perox・dase(HRP)" Jouynal of Electron Microscopy. 43. 119-121 (1994)
Watanabe, S 等人:“通过辣根过氧化物酶 (HRP) 对小鼠膀胱上皮的表面细胞进行选择性染色”,《电子显微镜杂志》43. 119-121 (1994)。
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Fuchigami M, Hyakumoto N, Miyazaki K, Nomura T and Sasaki J: "Texture and histological structure of carrots frozen at a programd rate and thawed in an electrostatic field." J Food Sci. 59. 1162-1167 (1994)
Fuchigami M、Hyakumoto N、Miyazaki K、Nomura T 和 Sasaki J:“以程序速率冷冻并在静电场中解冻的胡萝卜的质地和组织结构。”
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通讯作者:
Watanabe S, Kimura Y, Honda M, Sasaki J: "Selective staining of the superficial cells of mouse urinary bladder epithelium by horseradish peroxidase (HRP)" Journal of Electron Microscopy. 43. 119-121 (1994)
Watanabe S、Kimura Y、Honda M、Sasaki J:“辣根过氧化物酶 (HRP) 对小鼠膀胱上皮表面细胞的选择性染色”《电子显微镜杂志》。
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27
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