The expression of Mn-SOD in reproductive and endocrine organs.

Mn-SOD在生殖和内分泌器官中的表达。

• 批准号: 05670012
• 负责人: SASAKI Junzo
• 金额: $ 0.9万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670012/
• 关键词: Mn-SOD; Ovary; Uterus; Adenal gland; Hypophysectomy; In situ hybridization; Ovulation; Steroidogenesis; in situ ハイブリダイゼーション; 発情周期; 内卵胞膜細胞

It has been emphasized that superoxide dismutase (SOD) has a role as a scavenger enzyme. Previously, we reported that Mn-SOD were involved in steroidogenesis. In the present study, we examined the expression of Mn-SOD mRNA, in the female reproductive organs and endocrine organs of the rats under various experimental conditions using in situ hybridization technique and obtained the following results.1. Mn-SOD mRNA was expressed markedly in the theca interna cells during the early PMSG/hCG ovulatory process.2. Mn-SOD mRNA increased in the epithelial cells of the ovulated follicles and later, increased in luteal bodies.3. The expression of Mn-SOD mRNA in epithelial cells of endometrium began to increase by PMSG administration, reached maximum 6-24 h after hCG injection, and decreased 48 h after hCG injection.4. The level of Mn-SOD mRNA expression was shown to change during the estrous cycle. The marked expression was found in the cells of corpus albicans which contained hemoglobin metabolites.5. Estrogen induced the expression of Mn-SOD mRNA in the endometrium of immature rats.6. The expression of Mn-SOD in adrenal gland was localized to zona fasciculata and zona reticularis which showed active steroidogenesis.7. The expression of Mn-SOD mRNA decreased in adrenal gland, ovary and uterus of hypophysecto-mized rats.These results showed that Mn-SOD expression are deeply concerned in cellular metabolism including steroidogenesis.

超氧化物歧化酶（SOD）作为一种清除酶，其作用已被强调.以前，我们报道Mn-SOD参与类固醇合成。本研究采用原位杂交技术，检测了不同实验条件下大鼠雌性生殖器官和内分泌器官Mn-SOD mRNA的表达，得到以下结果. Mn-SOD mRNA在PMSG/hCG排卵早期卵泡内膜细胞中表达明显. Mn-SOD mRNA在排卵卵泡上皮细胞中表达增加，随后在黄体中表达增加. PMSG处理后，子宫内膜上皮细胞Mn-SOD mRNA表达开始增加，hCG处理后6-24 h达高峰，hCG处理后48 h下降. Mn-SOD mRNA的表达水平在发情周期中发生变化。在含有血红蛋白代谢产物的白小体细胞中有明显表达.雌激素可诱导未成熟大鼠子宫内膜Mn-SOD mRNA的表达. Mn-SOD在肾上腺中的表达主要集中在肾上腺皮质的束状核和网状核，表现出活跃的类固醇生成. Mn-SOD mRNA在垂体切除大鼠肾上腺、卵巢和子宫中的表达降低，提示Mn-SOD的表达与细胞代谢包括甾体激素的生成密切相关。

项目成果

Fuchigami M, Hyakumoto N, Miyazaki K, Nomura T and Sasaki J: "Texture and histological structure of carrots frozen at a programd rate and thawed in an electrostatic field." J Food Sci. 59. 1162-1167 (1994)

Fuchigami M、Hyakumoto N、Miyazaki K、Nomura T 和 Sasaki J：“以程序速率冷冻并在静电场中解冻的胡萝卜的质地和组织结构。”

Watanabe,S et al.: "Selective statng of the superficial cells of mouse urinary bladder epithelium by horseradisdr perox・dase(HRP)" Jouynal of Electron Microscopy. 43. 119-121 (1994)

Watanabe, S 等人：“通过辣根过氧化物酶 (HRP) 对小鼠膀胱上皮的表面细胞进行选择性染色”，《电子显微镜杂志》43. 119-121 (1994)。

Watanabe S, Kimura Y, Honda M, Sasaki J: "Selective staining of the superficial cells of mouse urinary bladder epithelium by horseradish peroxidase (HRP)" Journal of Electron Microscopy. 43. 119-121 (1994)

Watanabe S、Kimura Y、Honda M、Sasaki J：“辣根过氧化物酶 (HRP) 对小鼠膀胱上皮表面细胞的选择性染色”《电子显微镜杂志》。

Watanabe,H: "Electron microscopic study of the cytoskeleton of osteoblasts in rat alveolar bone." The Bulletin of Tokyo Dental College. 34. 89-94 (1993)

Watanabe，H：“大鼠牙槽骨中成骨细胞细胞骨架的电子显微镜研究。”

Sasaki,J.: "Detection of manganese superoxide dismufase(Mn-SOD)mRNA in rat ovary by in situ hybridization(ISH)." Acta Anatomica Nipponica. 68. 761-761 (1993)

Sasaki,J.：“通过原位杂交 (ISH) 检测大鼠卵巢中锰超氧化物歧化酶 (Mn-SOD) mRNA。”