The expression of Mn-SOD in reproductive and endocrine organs.

人们一直强调，超氧化物歧化酶(SOD)具有清道夫酶的作用。以前，我们曾报道过锰-超氧化物歧化酶参与类固醇的合成。本研究采用原位杂交技术，检测了不同实验条件下大鼠雌性生殖器官和内分泌器官中Mn-SOD mRNA的表达，获得了以下结果。结论：1.在PMSG/hCG排卵早期，内膜细胞中有明显的Mn-SOD基因表达。在排卵的卵泡上皮细胞中，锰-超氧化物歧化酶的mRNA表达增加，之后在黄体中的表达增加。注射PMSG后，子宫内膜上皮细胞中Mn-SOD mRNA的表达开始增加，6-24 h达高峰，48 h后表达下降。在发情周期中，锰-超氧化物歧化酶的表达水平发生了变化。在含有血红蛋白代谢物的白念珠菌细胞中有明显的表达。雌激素诱导未成熟大鼠子宫内膜锰-超氧化物歧化酶的表达。在肾上腺中，Mn-SOD的表达定位于束状带和网状带，表现出活跃的类固醇激素生成。在去垂体大鼠的肾上腺、卵巢和子宫中，锰-超氧化物歧化酶的表达减少，表明锰-超氧化物歧化酶的表达与细胞代谢包括类固醇激素的合成密切相关。

It has been emphasized that superoxide dismutase (SOD) has a role as a scavenger enzyme. Previously, we reported that Mn-SOD were involved in steroidogenesis. In the present study, we examined the expression of Mn-SOD mRNA, in the female reproductive organs and endocrine organs of the rats under various experimental conditions using in situ hybridization technique and obtained the following results.1. Mn-SOD mRNA was expressed markedly in the theca interna cells during the early PMSG/hCG ovulatory process.2. Mn-SOD mRNA increased in the epithelial cells of the ovulated follicles and later, increased in luteal bodies.3. The expression of Mn-SOD mRNA in epithelial cells of endometrium began to increase by PMSG administration, reached maximum 6-24 h after hCG injection, and decreased 48 h after hCG injection.4. The level of Mn-SOD mRNA expression was shown to change during the estrous cycle. The marked expression was found in the cells of corpus albicans which contained hemoglobin metabolites.5. Estrogen induced the expression of Mn-SOD mRNA in the endometrium of immature rats.6. The expression of Mn-SOD in adrenal gland was localized to zona fasciculata and zona reticularis which showed active steroidogenesis.7. The expression of Mn-SOD mRNA decreased in adrenal gland, ovary and uterus of hypophysecto-mized rats.These results showed that Mn-SOD expression are deeply concerned in cellular metabolism including steroidogenesis.