The expression of Mn-SOD in reproductive and endocrine organs.

超氧化物歧化酶（SOD）具有清除酶的作用。在此之前，我们报道了Mn-SOD参与类固醇生成。在本研究中，我们采用原位杂交技术检测了不同实验条件下大鼠雌性生殖器官和内分泌器官中Mn-SOD mRNA的表达，得到了以下结果：在PMSG/hCG早期排卵过程中，卵膜内细胞中Mn-SOD mRNA显著表达。2 . Mn-SOD mRNA在卵泡上皮细胞中表达升高，随后在黄体中表达升高。3 .经PMSG处理后，子宫内膜上皮细胞中Mn-SOD mRNA的表达开始升高，在hCG注射后6-24 h达到峰值，48 h下降。Mn-SOD mRNA的表达水平在发情周期中发生变化。在含有血红蛋白代谢物的白色体细胞中发现了明显的表达。雌激素诱导未成熟大鼠子宫内膜中Mn-SOD mRNA的表达。肾上腺中Mn-SOD的表达局限于束状带和网状带，具有活跃的类固醇生成作用。垂体化大鼠肾上腺、卵巢和子宫中Mn-SOD mRNA表达降低。这些结果表明，Mn-SOD的表达与细胞代谢包括类固醇生成密切相关。

It has been emphasized that superoxide dismutase (SOD) has a role as a scavenger enzyme. Previously, we reported that Mn-SOD were involved in steroidogenesis. In the present study, we examined the expression of Mn-SOD mRNA, in the female reproductive organs and endocrine organs of the rats under various experimental conditions using in situ hybridization technique and obtained the following results.1. Mn-SOD mRNA was expressed markedly in the theca interna cells during the early PMSG/hCG ovulatory process.2. Mn-SOD mRNA increased in the epithelial cells of the ovulated follicles and later, increased in luteal bodies.3. The expression of Mn-SOD mRNA in epithelial cells of endometrium began to increase by PMSG administration, reached maximum 6-24 h after hCG injection, and decreased 48 h after hCG injection.4. The level of Mn-SOD mRNA expression was shown to change during the estrous cycle. The marked expression was found in the cells of corpus albicans which contained hemoglobin metabolites.5. Estrogen induced the expression of Mn-SOD mRNA in the endometrium of immature rats.6. The expression of Mn-SOD in adrenal gland was localized to zona fasciculata and zona reticularis which showed active steroidogenesis.7. The expression of Mn-SOD mRNA decreased in adrenal gland, ovary and uterus of hypophysecto-mized rats.These results showed that Mn-SOD expression are deeply concerned in cellular metabolism including steroidogenesis.