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Expression of superoxide dismutase in cells of the female reproductive organs as well as malignant cells of these organs.

超氧化物歧化酶在女性生殖器官细胞以及这些器官的恶性细胞中的表达。

基本信息

批准号：
07671787
负责人：
SASAKI Junzo
金额：
$ 1.28万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

Reactive oxygen species have been shown to play critical roles in the pathogenesis of various diseases and the roles of superoxide dismutases (SOD) as scavenger enzyme have been emphasized in oxygen radical studies. In the present study, to investigate the role of Mn-SOD in ceuluar metabolism, expressin of Mn-SOD mRNA was examined in rat female reproductive organs and the following results were obtained.1. During the pregnant mare serum gonadotrophin (PMSG)/human chorionic gonadotrophin (HCG) -induced ovulatory process, and during the estrous cycle,(1) . Expression was observed in theca interna cells and granulosa cells of ovulating follicles before ovulation occurred, and in the corpora lutea after ovulation occurred. Expression of Mn-SOD mRNA in the ovary was noted in cells that initiate progesterone synthesis.(2) . Luminal epithelial cells showed maximal expression of Mn-SOD mRNA about 10-12 h after ovulation in the uterus. Uterine eosinophils which possess estrogen binding sites showed cyclic changes in strong expression of the transcripts.(3) . The expression level in the vagina changed after ovulatoin occurred.(4) . The epithelial cells in the oviduct did not express Mn-SOD mRNA throught the ovulatory process or the estrous cycle.2. Expresison in the uterus and vagina can be induced in immature rats by estradiol injection.These results suggest that expresison of Mn-SOD mRNA is involved in hormone-induced metabolic changes.We synthesize RNA probes for mice Mn-SOD mRNA and utilize for detecting Mn-SOD mRNA in vaginal epithelium in normal mice. Further studies using normal and acatalasemic mice which lack catalase activity are in progress. As RNA probes for human Mn-SOD mRNA have not been synthesized yet, studies using human malignant cells have been performed.

活性氧在多种疾病的发病机制中起着重要的作用，超氧化物歧化酶（SOD）作为清除氧自由基的酶，在氧自由基的研究中一直受到重视。本研究通过对雌性大鼠生殖器官Mn-SOD mRNA表达的检测，探讨Mn-SOD在细胞代谢中的作用.在孕马血清促性腺激素（PMSG）/人绒毛膜促性腺激素（HCG）诱导的排卵过程中，以及在发情周期中，（1）。排卵前在排卵卵泡的内膜细胞和颗粒细胞中表达，排卵后在黄体中表达。Mn-SOD mRNA在卵巢中的表达在启动孕酮合成的细胞中被注意到。(2) .在排卵后10-12 h，Mn-SOD mRNA在子宫腔上皮细胞中的表达达到高峰。具有雌激素结合位点的子宫嗜酸性粒细胞在转录物的强表达中表现出周期性变化。(3) .排卵后阴道组织中的表达水平发生变化。(4) .输卵管上皮细胞在整个排卵过程和发情周期均不表达Mn-SOD mRNA.雌激素可诱导未成年大鼠子宫和阴道组织中Mn-SOD mRNA的表达，提示Mn-SOD mRNA的表达参与了雌激素诱导的代谢变化，我们合成了小鼠Mn-SOD mRNA的RNA探针，并用于检测正常小鼠阴道上皮组织中Mn-SOD mRNA的表达。使用缺乏过氧化氢酶活性的正常小鼠和无酶血症小鼠的进一步研究正在进行中。由于人Mn-SOD mRNA的RNA探针尚未合成，因此已使用人恶性细胞进行了研究。