The cytoskeleton of Clara cell mammalian lung
克拉拉细胞哺乳动物肺的细胞骨架
基本信息
- 批准号:62570007
- 负责人:
- 金额:$ 1.28万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1987
- 资助国家:日本
- 起止时间:1987 至 1988
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Previously, the author reported the presence of abundant filaments in apical cytoplasmic protrusions (apical caps) of Clara cells of rats by perfusing their lungs with a medium containing Triton X-100 solution. This finding suggested that the apical cap was quint stable structure and was not apocrine droplet. Clara cells were widely distributed in the terminal bronchioles and in the upper airway and have the important role for the secretion of proteinous materials or oxidative metabolism of xenobiotics. The presence of filaments were investigated in several mammals and the nature and the genesis of the filaments were studied in rat lung.Filaments were found in apical caps of Clara cells of ret, mouse, guinea pig, hamster, steer, pig and human lung. The presence of these filaments can be utilized for identifying nonciliated bronchiolar epithelial cells in each airway. These filaments were in close association with cell membrane and formed homogeneous matrix just under the cell membrance … More .Human Clara cell with few smooth-surfaced endoplasmic reticulum have abundant filaments such as rat or mouse Clara cells.Most of these filaments were found to be actin by the use of heavy meromyosin (HMM). Some filaments which were not decorated with HMM were shown to be cytokeration by immunohistochemical method. Desmin, vimentin, neurofilament and GFAP were not found.The presence of filaments were investigated in pre- and postnatal rat. Clara cells of fetuses at the late gestation were distinguished from ciliated cells and contained a large amount of glycogen in the cytoplasm. After detergent treatment, filaments were not found in the cytoplasm. However, glycogen began to decrease rapidly and the presence of filaments was confirmed in the cell of a 1-day old rat. It has been shown that Clara cells are immature at birth and undergo marked early postnatal maturation. The present results suggest that the appearance of filaments also relates to the maturation of Clara cells and the release of granules. Less
以前,作者报道了通过用含有Triton X-100溶液的培养基灌注大鼠肺,在Clara细胞的顶端细胞质突起(顶端帽)中存在丰富的细丝。这一结果表明,顶帽是一种五端稳定结构,而不是顶泌液滴。Clara细胞广泛分布于终末细支气管和上呼吸道,在蛋白质物质的分泌和外源性物质的氧化代谢中起重要作用。本文研究了几种哺乳动物肺纤维的存在,并对大鼠肺纤维的性质和起源进行了研究。在大鼠、小鼠、豚鼠、仓鼠、阉牛、猪和人肺的Clara细胞顶帽中发现了纤维。这些细丝的存在可用于识别每个气道中的无纤毛细支气管上皮细胞。这些纤维与细胞膜紧密结合,在细胞膜下形成均匀的基质 ...更多信息 人Clara细胞具有丰富的微丝,其表面光滑的内质网较少,与大鼠或小鼠Clara细胞相似,这些微丝大多数是肌动蛋白。免疫组化染色显示部分未修饰的丝细胞角化。未发现结蛋白、波形蛋白、神经丝和胶质纤维酸性蛋白。妊娠晚期胎儿的Clara细胞与纤毛细胞不同,细胞质中含有大量糖原。去污剂处理后,在细胞质中没有发现细丝。然而,糖原开始迅速减少,并在1日龄大鼠的细胞中证实了细丝的存在。已经表明,克拉拉细胞在出生时是不成熟的,并且经历显著的出生后早期成熟。这些结果表明,纤维的出现也与Clara细胞的成熟和颗粒的释放有关。少
项目成果
期刊论文数量(17)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Junzo Sakaki: J.Electron Microsc.37. 38-44 (1988)
Junzo Sakaki:J.Electron Microsc.37。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sadahiro Watanabe: "Encapsulation of the small specimens for electron microscopy with the use of low gel temperature agarose." J. Electron Microsc.37. 89-91 (1988)
Sadahiro Watanabe:“使用低凝胶温度琼脂糖封装用于电子显微镜的小样本。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Junzo Sasaki.: A cta Med.Okayama. 42. 193-200 (1988)
Junzo Sasaki.:A cta Med.Okayama。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Junzo Sasaki: Okajimas Folia Anat.Jpn.65. 155-170 (1988)
佐佐木纯三:Okajimas Folia Anat.Jpn.65。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
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SASAKI Junzo其他文献
SASAKI Junzo的其他文献
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{{ truncateString('SASAKI Junzo', 18)}}的其他基金
Identification of osteoblast function regulating factor secreted by human mesenchymal stem cell
人间充质干细胞分泌的成骨细胞功能调节因子的鉴定
- 批准号:
21591945 - 财政年份:2009
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Quantification of transcripts in testes preparation and its application to other tissue sections
睾丸制备中转录物的量化及其在其他组织切片中的应用
- 批准号:
18591801 - 财政年份:2006
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of gene expression in the rat testis during exposure to endocrine-disrupting chemicals
暴露于内分泌干扰化学物质期间大鼠睾丸的基因表达分析
- 批准号:
15591748 - 财政年份:2003
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
How reactive oxygen species mediate the effects of endocrine-disrupting agents on the reproductive organs?
活性氧如何介导内分泌干扰剂对生殖器官的影响?
- 批准号:
13671718 - 财政年份:2001
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Ovulation and superoxide
排卵和超氧化物
- 批准号:
09671683 - 财政年份:1997
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Expression of superoxide dismutase in cells of the female reproductive organs as well as malignant cells of these organs.
超氧化物歧化酶在女性生殖器官细胞以及这些器官的恶性细胞中的表达。
- 批准号:
07671787 - 财政年份:1995
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The expression of Mn-SOD in reproductive and endocrine organs.
Mn-SOD在生殖和内分泌器官中的表达。
- 批准号:
05670012 - 财政年份:1993
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
The Observation of the cells expressing superoxide dismutase mRNA by in situ hybridization.
原位杂交观察细胞表达超氧化物歧化酶mRNA。
- 批准号:
02670011 - 财政年份:1990
- 资助金额:
$ 1.28万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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