Effect of poly(ADP-ribose) synthetase on the expression of MHC class II molecules.

聚（ADP-核糖）合成酶对 MHC II 类分子表达的影响。

• 批准号: 05670148
• 负责人: TANIGUCHI Taketoshi
• 金额: $ 1.34万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670148/
• 关键词: MHC class II; Gene Regulation; Poly (ADP-ribose); Fusion Protein; Macrophage; B Lymphocyte; MHC class II; ポリADP-リボース; マクロファージ; ポリADPR; HLA-DR; IFNgamma; Reporter gene; 遺伝子調節; アンチセンスRNA; Promoter

We have demonstrated that the high expression of poly (ADP-ribose) synthetase (PARS) caused the depression of MHC class II molecules. In B cell lines, which constitutively express the class II molecule, there are significant amounts of PARS.Thus, we attempted to detect a cell type-specific trans-acting factor which interacted with PARS and regulated the MHC class II gene.I.We constructed a fusion protein by using the automodification and DNA binding domains of PARS.We labeled nuclear proteins with ^<35>S-Met in B cells and macrophage cells and the ^<35>S-labeled nuclear proteins were incubated with the PARS-fusion protein. We detected a 45kDa and 35kDa binding partner for PARS in B cells.II.We prepared a probe DNA of human MHC class II genomic DNA in the region of DNase I hypersensitive site II(DHSII). We prepared nuclear proteins from class II-positive cells, class II-inducible cells and negative cells and analyzed them by South-western blotting by the use of the DHS II DNA probe. We detected a 35kDa nuclear protein which interacted with the DNA probe. Whether or not the DHS II-associated 35kDa protein interacts with PARS is still under investigation.

我们已经证明，高表达的聚（ADP-核糖）合成酶（PARS）引起的抑制MHC II类分子。在组成型表达MHC Ⅱ类分子的B细胞系中，存在大量的PARS，因此，我们试图检测与PARS相互作用并调节MHC Ⅱ类基因的细胞类型特异性反式作用因子<35><35>。我们在B细胞中检测到PARS的45 kDa和35 kDa的结合伴侣。II.我们在DNase I超敏位点II（DHS II）区域制备了人MHC II类基因组DNA的探针DNA。我们从II类阳性细胞、II类诱导细胞和阴性细胞制备核蛋白，并通过使用DHS II DNA探针的South-western印迹法对其进行分析。我们检测到一个35 kDa的核蛋白与DNA探针相互作用。DHS II相关的35 kDa蛋白是否与PARS相互作用仍在研究中。

项目成果

Matsuura,Yasushi et al.: "Class II-Restricted Presentation of An Immunoglobulin Heavy-Chain-Gene Product by A Gene-Transfected B-cell Line." Jpn.J.Med.Sci.Biol.47. 195-210 (1994)

Matsuura, Yasushi 等人：“基因转染的 B 细胞系对免疫球蛋白重链基因产物的 II 类限制性呈现。”

Takeo Imai, Katsuya Ohta, Hiromitsu Kigawa, hiromi Kanoh, Taketoshi Taniguchi, And Jiro Tobari: "Preparation of High-Molecular-Weight DNA : Application to Mycobacterial Cells." Anal.Biochem.222. 479-482 (1994)

Takeo Imai、Katsuya Ohta、Hiromitsu Kikawa、hiromi Kanoh、Taketoshi Taniguchi 和 Jiro Tobari：“高分子量 DNA 的制备：在分枝杆菌细胞中的应用”。

Tomoda,Takashi et al.: "Imbalance of the interleukin 2 system in children with IDDM." Diabetologia. 37. 476-482 (1994)

Tomoda,Takashi 等人：“IDDM 儿童白细胞介素 2 系统失衡。”

Imai,Takeo et al.: "Preparation of High-Molecular-Weight DNA;Application to Mycobacterial Cells." Anal,Biochem,. 222. 479-482 (1994)

Imai, Takeo 等人：“高分子量 DNA 的制备；在分枝杆菌细胞中的应用。”

Taniguchi,T.,Nemoto,Y.,Ota,K.,Imai,T.& Tobari,J.: "Participation of poly (ADP-ribose) synthetase in the process of norepinephrineinduced inhibition of MHC class II antigen expression in astrocytoma cells." Biochem. Biophys. Res. Commun.193. 886-889 (1993)

谷口 T.、根本 Y.、太田 K.、今井 T.