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Regulation of Gene Expression by Poly(ADP-ribosyl)ation

聚（ADP-核糖基）化调节基因表达

基本信息

批准号：
60570117
负责人：
TANIGUCHI Taketoshi
金额：
$ 0.96万
依托单位：
Kochi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570117/
关键词：
Poly(ADP-ribose)Gene Regulation 細胞分化

项目摘要

Poly(ADP-ribose) syntheate is associated with a chromatin and some nuclear proteins are poly(ADP-ribosyl)ated by the enzyme. However, the physiological role of the modification is still unclear. In order to examine tne effect of poly(ADP-ribosyl)ation on gene expression we first established a method to measure the amount of the enzyme in tissues and cells by the antibody against the enzyme. We determined the change in the amount of the enzyme during the neural differenciation of rat chromafin cells induced by nerve growth factor. The level of the enzyme was decreased during the process. Synthesis of uteroglobin in rabbit uterine epithelial cells during early pregnancy is induced by progesterone. We attempted to determine the change in the amount of. the enzyme during the uteroglobin induction process. However, the level of the enzyme was not large enough to determine by the antibody method. Thus, we isolated a cDNA clone coding for the enzyme. We use the cDNA as a probe to estimate the level of mRNA for the enzyme during the differenciation of murine macrophage tumor cell line. The amount of mRNA for the enzyme decreased to 20% of that for the untreated cells by 2 days after incubation in the presence of <gamma> -interferon, while the Ia antigen. one of the histocompartibility gene products, was markedly induced. We conclude that the enzyme in somehow involved in the gene regulation. especially during differnciation.

聚（ADP-核糖）合成物与染色质相关，一些核蛋白被该酶聚（ADP-核糖基）化。然而，修饰的生理作用仍不清楚。为了检查聚（ADP-核糖基）化对基因表达的影响，我们首先建立了一种通过抗酶的抗体来测量组织和细胞中酶的量的方法。我们测定了神经生长因子诱导大鼠嗜铬细胞神经分化过程中酶量的变化。在此过程中酶的水平降低。妊娠早期兔子宫上皮细胞中子宫珠蛋白的合成是由黄体酮诱导的。我们试图确定金额的变化。子宫珠蛋白诱导过程中的酶。然而，酶的水平不足以通过抗体方法测定。因此，我们分离了编码该酶的cDNA克隆。我们使用 cDNA 作为探针来估计小鼠巨噬细胞肿瘤细胞系分化过程中酶的 mRNA 水平。在存在<γ>-干扰素和Ia抗原的情况下孵育2天后，酶的mRNA量减少至未处理细胞的mRNA量的20%。组织相容性基因产物之一被显着诱导。我们得出结论，该酶在某种程度上参与了基因调控。特别是在微分过程中。