Physical Activity and Immune Senescene in Men

一项横断面调查研究了习惯性耐力锻炼者在老年时是否比久坐不动的人保留了更高水平的T细胞功能。受试者均为男性，包括17名老年跑步者，16名年轻对照者和19名老年对照者（平均年龄<正负> SD: 63.8 <正负> 3.3,23.6 <正负> 1.6,65.8 <正负> 3.5，年）。运动后至少36小时采集外周血样本。仅对非吸烟者的数据进行组间比较，采用单因素方差分析和事后多重比较的Bonferroni调整。淋巴细胞功能被评估为每个主要应答细胞的调整活性以及通常未调整的活性。与年轻受试者相比，两组老年人的循环CD3^+和CD8^+计数均较低（组效应分别为P= 0.029和P= 0.001）， CD4/CD8比值有升高趋势，但活化CD3^+和“记忆”CD4^+和CD8^+细胞百分比较高（均P< 0.001）。老年人对植物血凝素（PHA）、美洲商陆有丝分裂原（PWM）和同种异体抗原（混合淋巴细胞反应）的增殖反应明显降低（未调整活性，P< 0.001， P< 0.001和P= 0.024）。老年久坐者IL-2的产生倾向于降低（P= 0.058 vs年轻受试者）。然而，自然杀伤细胞活性并未受损，il -1 β、ifn - γ和IL-4的产生在久坐的老年人中保持不变。活动组和久坐组之间的比较显示，免疫活性细胞的循环计数没有差异。然而，活跃的老年受试者对PHA（未调整为P= 0.016，调整为P= 0.034）、PWM（分别为P= 0.011和P= 0.040）、IL-2 （P= 0.021）、ifn - γ (P= 0.015)和IL-4 （P= 0.012）的增殖反应显著增强。这些结果表明，晚年的耐力训练与循环T细胞功能和相关细胞因子产生的某些方面的年龄相关下降有关。少

A cross-sectional survey examined whether habitual endurance exercisers retained a higher level of T cell function than sedentary individuals in old age. Subjects, all male, comprised 17 elderly runners, 16 young controls and 19 elderly controls (mean ages <plus-minus> SD : 63.8 <plus-minus> 3.3,23.6 <plus-minus> 1.6, and 65.8 <plus-minus> 3.5, yr, respectively) . Samples of peripheral blood were collected at least 36 h after exercise. Group comparisons were made on data from non-smokeres only, using one-way ANOVA and a Bonferroni adjustment for post-hoc multiple comparisons. Lymphocyte functions were assessed as adjusted activity per major responding cell as well as the usual unadjusted activity. In comparison wtih the young subjects, both elderly groups had lower circulating CD3^+ and CD8^+ counts (group effects : P=.029, P=.001, respectively) , with a trend to a higher CD4/CD8 ratio, but higher percentages of activated CD3^+, and "memory" CD4^+ and CD8^+ cells (all, P<.001) . Prolif … More erative responses to phytohemagglutinin (PHA) , pokeweed mitogen (PWM) and alloantigens (mixed lymphocyte reaction) were markedly reduced in the elderly (for unadjusted activities, P<.001, P<.001 and P=.024, respectively). IL-2 production tended to be decreased in the elderly sedentary subjects (P=.058 vs young subjects) . However, natural killer cell activity was not impaired, and IL-1beta, IFN-gamma and IL-4 production remained unchanged in the elderly sedentary subjects. Comparison between the active and sedentary elderly groups showed no differences in circulating counts of immunocompetent cells. However, the active elderly subjects demonstrated significantly greater proliferative responses to PHA (P=.016 for unadjusted and P=.034 for adjusted activity) , and to PWM(P=.011 and P=.040, respectively) , and higher rates of IL-2 (P=.021), IFN-gamma (P=.015) and IL-4 production (P=.012) . These results suggest that endurance training in later life is associated with a lesser age-related decline in certain aspects of circulating T cell function and related cytokine production. Less