The effect of platelet activating factor on mucociliary regulation in airway epithelium cultured at airinterface

血小板活化因子对气界面培养气道上皮粘膜纤毛调节的影响

• 批准号: 05670538
• 负责人: KONDO Mitsuko
• 金额: $ 1.34万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670538/
• 关键词: platelet activating factor; ion transport; calcium ion; inositol triphosphate; prostaglandin; air interface; airway epithelial cells; プロスタグランデイン; air interface; カルシウム

To elucidate the effect of platelet activating factor on mucociliary transport of airway epithelial cells, we studied bioelectric properties of cow tracheal epithelial cells cultured at an airinterface. PAF dose-dependently increased short-circuit current and conductance. This effect was inhibited by Cl channel blocker, DPAC,but not Na channel blocker, amiloride. In addition, this effect was inhibited by the pretreatment of PAF antagonist, BAPTA-AM,and indomethacin, suggesting that PAF increases Cl secretion via PAF receptor, calcium and prostaglandin dependent pathway. Therefore, to elucidate the signal transduction of PAF in the airway epithelial cells, we measured second messengers.In fura2-loaded cells, PAF increased [Ca^<2+>]i in a dose-dependent manner. This [Ca^<2+>]i response consisted of a transient increase and a subsequent sustained elevation. Ca^<2+>-free medium totally abolished the sustained response, but it only inhibited the transient response. Moreover, PAF increased inositol triphosphate level whose time course was similar to the transient calcium response. PAF also increased PGE2 and PGF2alpha by RIA assay.These results suggest that PAF-induced epithelial function is mediated by the increased calcium and prostaglandins.

为了阐明血小板活化因子对气道上皮细胞粘液纤毛转运的影响，我们研究了在空气界面上培养的牛气管上皮细胞的生物电特性。PAF剂量依赖性地增加短路电流和电导。Cl通道阻断剂DPAC可抑制此作用，而Na通道阻断剂阿米洛利则不抑制此作用。PAF拮抗剂BAPTA-AM和吲哚美辛预处理可抑制这种作用，提示PAF通过PAF受体、钙离子和前列腺素依赖性途径增加Cl分泌。因此，为了阐明PAF在气道上皮细胞中的信号转导，我们检测了第二信使，在fura 2负载的细胞中，PAF以剂量依赖的方式增加[Ca^<2+>]i。这种[Ca^<2+>]i反应包括短暂升高和随后的持续升高。无Ca^<2+>培养基完全消除持续性反应，但仅抑制瞬时反应. PAF还能增加三磷酸肌醇的含量，其时间过程与瞬时钙反应相似。结果表明，PAF诱导的上皮细胞功能变化是由钙离子和前列腺素介导的。

项目成果

M. Kondo: "The effect of platelet activating factor on intracellular free calcium in cow tracheal epithelium" American Journal of Respiratory Cell and Molecular Bilogy. 10. 279-283 (1994)

M. Kondo：“血小板激活因子对牛气管上皮细胞内游离钙的影响”美国呼吸细胞和分子生物学杂志。

M.Kondo: "The effect of platelet activating factor on intracellular free calcium in cow tracheal epithelium" Am.J.Respir.Cell Mol.Biol. 10(in press). (1994)

M.Kondo：“血小板激活因子对牛气管上皮细胞内游离钙的影响”Am.J.Respir.Cell Mol.Biol。

近藤光子: "気道粘膜における細胞間相互作用-上皮イオントランスポートに対する好酸球の影響-" 日本胸部疾患学会雑誌. 32. 1067-1072 (1994)

Mitsuko Kondo：“气道粘膜中的细胞间相互作用 - 嗜酸性粒细胞对上皮离子转运的影响”日本胸部疾病学会杂志 32. 1067-1072 (1994)。

M.Kondo et al: "Cell-to Cell Interaction in Airway Mucosa-Effect of Eosinophils on Airway Epithelial Ion Transport" The Japanese Joural of Thoracic Diseases. 32. 1067-1072 (1994)

M.Kondo 等人：“气道粘膜中的细胞间相互作用 - 嗜酸性粒细胞对气道上皮离子转运的影响”，日本胸部疾病杂志。

M.Kondo et al: "The effect of platelet activating factor on intracellular free calcium in cow tracheal epithelium" American Journal of Respiratory Cell and Molecular Biology. 10. 279-283 (1994)

M.Kondo 等人：“血小板激活因子对牛气管上皮细胞内游离钙的影响”美国呼吸细胞与分子生物学杂志。