Regulation of Na-pump gene expression in cardiovascular system

心血管系统钠泵基因表达的调控

• 批准号: 05670632
• 负责人: IKEDA Uichi
• 金额: $ 1.34万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670632/
• 关键词: sodium pump; Na, K-ATPase; cardiac myocyte; angiotensin II; 転写因子; Na, K-ATPase; 高血圧; 心肥大

Na, K-ATPase, also called the sarcolemmal Na^+-pump, is a ubiquitous transmembrane protein of mammalian cells that maintains electrochemical Na^+ and K^+ gradients across the plasma membrane and affects excitability, contractility, and cell volume regulation of various kinds of cells. The Na, K-ATPase protein comprises two subunits : a large catalytic alpha-subunit and a smaller glycosylated beta-subunit of unknown function. At least three alpha-subunit isoforms, alpha1, alpha2, and alpha3, have been characterized in rats and humans. Two beta-subunit isoforms, beta1 and beta2, have been identified in rats.In the present research project, we investigated the effects of sodium ion and various hormones on Na, K-ATPase alpha1 and beta1 mRNA expression in the cardiovascular system. Na, K-ATPase mRNA expression was analyzed by Northern blotting and Na^+-pump protein content was analyzed by enzyme-linked immunosorbent assay (ELISA). We have revealed that sodium ion, thyroid hormone, and angiotensin II stimulate Na, K-ATPase alpha1-and beta1-isoform mRNA and protein expression in rat vascular smooth muscle cells, mesangial cells, and cardiac myocytes and regulate contractility, excitability, and volume regulation of these cells.

Na， K- atp酶，也称为肌层Na^+泵，是哺乳动物细胞中普遍存在的跨膜蛋白，维持Na^+和K^+在质膜上的电化学梯度，影响各种细胞的兴奋性、收缩性和细胞体积调节。Na， k - atp酶蛋白包括两个亚基：一个较大的催化α亚基和一个较小的功能未知的糖基化β亚基。至少有三种α -亚基亚型，α 1、α 2和α 3，已经在大鼠和人类中被发现。两种β亚基异构体，β a1和β a2，已经在大鼠中被鉴定出来。在本研究项目中，我们研究了钠离子和各种激素对心血管系统Na、k - atp酶α 1和β 1 mRNA表达的影响。Northern blotting法检测Na、k - atp酶mRNA表达，酶联免疫吸附法（ELISA）检测Na^+泵蛋白含量。我们发现，钠离子、甲状腺激素和血管紧张素II刺激大鼠血管平滑肌细胞、系膜细胞和心肌细胞中Na、k - atp酶α 1和β 1异构体mRNA和蛋白的表达，并调节这些细胞的收缩性、兴奋性和体积调节。

项目成果

U.Ikeda: "Ouabain enhances nitric oxide synthesis in rat vascular smooth muscle cells induced by interleukin-1beta." Eur.J.Phamacol.288. 379-383 (1995)

U.Ikeda：“哇巴因可增强白细胞介素 1β 诱导的大鼠血管平滑肌细胞中的一氧化氮合成。”

Uichi Ikeda: "Neutrophil adherence to rat cardiac myocyte by pro-inflammatory cytokines." J.Cadiovasc.Pharmacol. (印刷中). (1994)

Uichi Ikeda：“中性粒细胞通过促炎细胞因子粘附于大鼠心肌细胞。”J.Cadiovasc.Pharmacol（出版中）。

Yoshio Tsuruya: "Decreased Na,K-ATPase gere expression in carodionyopathic hamster hearts" Life Sciences. 54. 71-77 (1994)

Yoshio Tsuruya：“心血管病仓鼠心脏中 Na,K-ATP 酶表达降低”生命科学。

Uichi Ikeda: "Expression of ixtarcellular adhesion molecule-1 on rat vasiular smooth miscle cells by pro-inflammatoy cytokines" Atheroscleosis. 104. 61-68 (1993)

Uichi Ikeda：“促炎细胞因子在大鼠血管平滑肌细胞上表达 ixtarcellular 粘附分子-1”动脉粥样硬化。

Keiji Yamamoto: "Endothelin Production in Pulmanary Circulalion of Patients with Mitral Stenosis" Ciculation. (印刷中). (1994)

Keiji Yamamoto：“二尖瓣狭窄患者肺循环中的内皮素生成”（出版中）。