Therapeutic angiogenesis to ischemic heart disease by cell transplantation

通过细胞移植治疗缺血性心脏病的血管生成

• 批准号: 16390220
• 负责人: IKEDA Uichi
• 金额: $ 8.83万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390220/
• 关键词: regeneration; atherosclerosis; myocardial infarction; bone marrow; angiogenesis

We have previously reported the effectiveness and safety of therapeutic angiogenesis by transplantation of autologous bone marrow mononuclear cells to ischemic limbs (Lancet 2002). In this study, we investigated whether transplantation of bone marrow-derived endothelial progenitor cells (EPCs) would also be effective in patients with ischemic heart disease. Bone marrow-derived EPCs were transplanted to the myocardium in patients with ischemic heart disease ; They received bone marrow-derived CD34^+ cell transplantation into ungraftable regions of the left ventricle with concomitant coronary artery bypass grafts (hybrid therapy). Before and 1 month after cell transplantation, we assessed the regional blood flow by single photon emission computed tomography (SPECT). The blood flow in the EPC-transplanted region as well as the grafted region was significantly increased at 1 month after cell transplantation. No side effects were observed. In this study, we demonstrated that autologous transplantation of bone marrow-derived EPCs represents a new and promising strategy for clinical application designed to revascularize the ischemic myocardium

我们以前曾报道过自体骨髓单核细胞移植到缺血肢体的治疗性血管生成的有效性和安全性（Lancet 2002）。在这项研究中，我们调查了骨髓来源的内皮祖细胞（EPCs）移植是否也对缺血性心脏病患者有效。将骨髓来源的EPCs移植到缺血性心脏病患者的心肌中;他们接受骨髓来源的CD34 ^+细胞移植到左心室的不可移植区域，同时接受冠状动脉旁路移植术（混合疗法）。在细胞移植前和移植后1个月，通过单光子发射计算机断层扫描（SPECT）评估局部血流。EPC移植区域以及移植区域的血流量在细胞移植后1个月显著增加。未观察到副作用。在这项研究中，我们证明了骨髓来源的EPCs的自体移植代表了一种新的和有前途的临床应用策略，旨在使缺血心肌再血管化

项目成果

Interleukin-1beta attenuates beta-very low-density lipoprotein uptake and its receptor expression in vascular smooth muscle cells.

Interleukin-1beta 减弱血管平滑肌细胞中 β-极低密度脂蛋白的摄取及其受体表达。

• 发表时间: 2005
• 期刊: J Mol Cell Cardiol 38
• 作者: Takahashi M;Takahashi S;et. al.
• 通讯作者: et. al.

Changes in cardiac lipid metabolism during sepsis: The essential role of very low-density lipoprotein receptors

• DOI: 10.1016/j.cardiores.2005.11.014
• 发表时间: 2006-02-01
• 期刊: CARDIOVASCULAR RESEARCH
• 影响因子: 10.8
• 作者: Jia, LJ;Takahashi, M;Ikeda, U
• 通讯作者: Ikeda, U

Effective hepatocyte transplantation using rat hepatocytes with low asialoglycoprotein receptor expression

• DOI: 10.1016/s0002-9440(10)63315-9
• 发表时间: 2004-08-01
• 期刊: AMERICAN JOURNAL OF PATHOLOGY
• 影响因子: 6
• 作者: Ise, H;Nikaido, T;Ikeda, U
• 通讯作者: Ikeda, U

Repair of infarcted myocardium mediated by transplanted bone marrow-derived CD34+ stem cells in a nonhuman primate model

• DOI: 10.1634/stemcells.2004-0200
• 发表时间: 2005-03-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Yoshioka, T;Ageyama, N;Hanazono, Y
• 通讯作者: Hanazono, Y

Cardiac overexpression of MCP-1 in transgenic mice prevents cardiac dysfunction and remodeling after myocardial infarction.

转基因小鼠心脏中 MCP-1 的过度表达可预防心肌梗塞后的心脏功能障碍和重构。

• 发表时间: 2006
• 期刊: Circ Res 99
• 作者: Morimoto H;Takahashi M;Izawa A;Ise H;Hongo M;Kolattukudy PK;Ikeda U
• 通讯作者: Ikeda U