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LONG-TERM CLINICAL APPLICATION OF WEARABLE ARTIFICIAL ENDOCRINE PANCREAS - THE DEVELOPMENT OF A NEEDLE - TYPE GLUCOSE SENSOR COVERED WITH PHOSPHOLIPID MEMBRANE -

可穿戴式人工内分泌胰腺的长期临床应用——磷脂膜覆盖的针式血糖传感器的研制——

基本信息

批准号：
05670872
负责人：
SAKAKIDA Michiharu
金额：
$ 1.34万
依托单位：
Kumamoto University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

To prepare the long-life and stable glucose sensor, we developed the ferrocene-mediated needletype glucose sensor covered with newly designed biocompatible membrane, 2-methacryloyloxyethyl phosphorylcholine-co-n butyl methacrylate (MPC-co-BMA) membrane. In this membrane, the hydrophilic phosphorylcholine chains were grafted on the hydrophobic polymer surface. 1.The ferrocene-mediated needle-type glucose senso covered with poly (MPC-co-BMA) membrane achieved excellent results in vitro. Subcutaneous tissue blucose concentrations were measured in a wide range from 30 to more than 400 mg/dl. The correlation between subcutaneous tissue (Y) and blood (X) glucose concentrations was Y = 1.04X + 0.12 (r=0.98). The subcutaneous tissue glucose concentrations could be monitored precisely for 7 days without any in vivo calibrations, and for 14 days by introducting in vivo calibrations. 2.In ambulatory diabetic patients, physiological glycemic regulations could be acheived by application of wearable artificial endocrine pancreas with this ferrocene-mediated needle-type glucose sensor covered with poly (MPC-co BMA) membrane for 12 days by replacing the sensor every 5th day to avoid local infection at the site of sensor insertion. We therefore conclude that this sensor is stable and reliable, as compared to any other glucose sensors we developed.

为了制备长寿命、稳定的葡萄糖传感器，我们研制了一种二茂铁介导的针型葡萄糖传感器，该传感器表面覆盖有新设计的生物相容性膜2-甲基丙烯酰氧乙基磷酰胆碱-co-甲基丙烯酸正丁酯（MPC-co-BMA）膜。在该膜中，亲水性的磷酸胆碱链被接枝到疏水性的聚合物表面。1.二茂铁介导的聚（MPC-co-BMA）膜覆盖的针型葡萄糖传感器在体外取得了良好的效果。皮下组织蓝糖浓度的测量范围从30到超过400毫克/分升。皮下组织葡萄糖浓度（Y）与血液葡萄糖浓度（X）的相关性为Y = 1.04X + 0.12（r=0.98）。皮下组织葡萄糖浓度可以精确地监测7天而无需任何体内校准，并且通过引入体内校准可以精确地监测14天。2.在非卧床糖尿病患者中，使用聚（MPC-co BMA）膜覆盖的二茂铁介导的针型葡萄糖传感器的可穿戴式人工内分泌胰腺，通过每5天更换传感器以避免传感器插入部位的局部感染，可以实现生理性血糖调节，持续12天。因此，我们得出结论，与我们开发的任何其他葡萄糖传感器相比，该传感器稳定可靠。