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Cell kinetics of bone and soft tissue tumors

骨和软组织肿瘤的细胞动力学

基本信息

批准号：
05671211
负责人：
MATSUMOTO Keiji
金额：
$ 1.47万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

项目摘要

Human bone and soft tissue tumors were doubly stained with markers of proliferating cells (proliferating cell nuclear antigen, bromodeoxyuridine and iododeoxyuridine) and some kind of tumor markers including S-100, osteocalcin, prolyl hydroxylase. The purpose of this study is to investigate the relationship between cell growth and differentiation of tumor cells using immunohistochemical technique. The results were follows :(1) The main proliferative portion in dedifferentiated chondrosarcoma is dedifferentiated area that is S-100 protein negative.(2) The main proliferative cells in malignant fibrous histiocytoma of bone and soft tissue are undifferentiated fibroblast-like cells that are prolyl hydroxylase positive.(3) The main proliferative cells in liposarcoma are S-100 negative tumor cells.(4) In benign cartilaginous tumors S-100 positive chndroblastic cells have little ability of proliferation.(5) The main proliferative cells in osteosarcoma are osteocalcin positive osteoblast-like cells while chondrocyte-like cells and giant cells have little ability to proliferate.(6) Periosteal osteosarcoma is characterized by the proliferation of osteoblastic stromal cells, whereas parosteal chondrosarcoma is characterized with proliferation of chondroblastic tumor cells.(7) Duration of S period in cell cycle is measured with the transplanted sarcoma cells in nude mice. It could be a logical predictor of prognosis in bone and soft tissue sarcomas.(8) Measurement of S period duration under treatment of anti-cancer drugs showed chemosensitivity of the tumor cells. This method could be a clinical chemosensitivity test in practical use.

用增殖细胞标志物（增殖细胞核抗原、溴脱氧尿苷、碘脱氧尿苷）和S-100、骨钙素、脯氨酰羟化酶等肿瘤标志物对人骨组织和软组织肿瘤进行双重染色。本研究的目的是利用免疫组织化学技术探讨肿瘤细胞生长与分化的关系。结果表明：(1)去分化软骨肉瘤的主要增殖部位为S-100蛋白阴性的去分化区。(2)骨和软组织恶性纤维组织细胞瘤的主要增殖细胞为脯氨酸羟化酶阳性的未分化成纤维细胞样细胞。(3)脂肪肉瘤的增殖细胞以S-100阴性肿瘤细胞为主。(4)良性软骨肿瘤中S-100阳性软骨母细胞增殖能力弱。(5)骨肉瘤的主要增殖细胞是骨钙素阳性的成骨细胞样细胞，而软骨细胞样细胞和巨细胞的增殖能力较弱。(6)骨膜骨肉瘤以成骨基质细胞增生为特征，而骨旁软骨肉瘤以成软骨肿瘤细胞增生为特征。(7)用裸鼠移植的肉瘤细胞测量细胞周期S期的持续时间。它可能是骨和软组织肉瘤预后的一个合乎逻辑的预测因子。(8)测定抗癌药物作用下的S周期持续时间，显示肿瘤细胞的化学敏感性。该方法可作为临床实际应用的化学敏感性试验。