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ACTION OF NITRIC OXIDE ON THE INTERNAL URETHRAL SPHINCTER FUNCTION

一氧化氮对尿道内括约肌功能的作用

基本信息

批准号：
05671297
负责人：
NISHIZAWA Osamu
金额：
$ 1.15万
依托单位：
AKITA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671297/
关键词：
Nitric oxide Internal urethral sphincter Urethral relaxation 膀胱尿道 Nitric oxide

项目摘要

The present study was performed to investigate action of nitric oxide on the internal urethral sphincter function. Female rats were anesthetzed with urethane. The bladder neck was ligated in order to measure independently intravesical pressure and internal urethral pressure. Under condition of abolishment of the urethral striated muscle activity by the intravenous administration of pancronium bromide, the bladder and urethral pressure were continuosly recorded during rhythmic isovolumetric bladder contractions before and after intraarterial administration of the following drugs. Used drugs were L-arginine from which nitric oxide is synthetized, L-NMMA,L-NAME which inhibit the synthesis of nitric oxide from L-arginine, and methylene blue which inhibits the activation of soluble guanylyl cyclase. Before the administration of each drug, rhythmic bladder contractions with concomitant urethral relaxations were occurred. Administration of L-arginine produced a decrese in urethral pressure. A … More dministration of L-NMMA,L-NAME and methylene blue produced an inhibition of the internal urethral sphincter relaxation during rhythmic bladder contractions. The nitric oxide/cyclic GMP pathway seems to take an important role for the internal urethral sphincter relaxation.Decerebrate dogs were used to examine the effect of L-arginine and methylene blue on the isovolumetric bladder contractions in response to bladder fillig and electrical stimulation of pontine micturition center. In the isovolumetric bladder contractions in response to bladder filling, L-arginine produced an increase in bladder capacity and a decrease in bladder contraction pressure. Methylene blue produced an inhibition of effect of L-arginine. In the isovolumetric bladder contractions in response to electrical stimulation of pontine micturition center, methylene blue produced an increase in the bladder contraction pressure and duration of bladder contraction. The nitric oxide/cyclic GMP pathway seems to take certain role for bladder activity. Less

本研究旨在探讨一氧化氮对尿道内括约肌功能的影响。用乌拉坦麻醉雌性大鼠。结扎膀胱颈以独立测量膀胱内压和尿道内压。在静脉注射潘可溴铵（Pancronium bromide）消除尿道横纹肌活动的条件下，连续记录动脉注射下列药物前后膀胱节律性等容收缩时的膀胱和尿道压力。使用的药物是合成一氧化氮的L-精氨酸、抑制L-精氨酸合成一氧化氮的L-NMMA、L-NAME和抑制可溶性鸟苷酸环化酶活化的亚甲蓝。每种药物给药前，发生节律性膀胱收缩伴尿道松弛。应用L-精氨酸可使尿道压力下降。一 ...更多信息 L-NMMA、L-NAME和亚甲蓝对膀胱节律性收缩时的内尿道括约肌松弛有抑制作用。用去脑犬观察了L-精氨酸和亚甲蓝对膀胱充盈和电刺激桥脑排尿中枢引起的等容膀胱收缩的影响。在等容膀胱收缩膀胱充盈，L-精氨酸产生膀胱容量的增加和膀胱收缩压力的降低。亚甲蓝对L-精氨酸的作用有抑制作用。在电刺激脑桥排尿中枢引起的等容膀胱收缩中，亚甲蓝使膀胱收缩压和收缩持续时间增加。一氧化氮/环鸟苷酸途径似乎对膀胱活动起一定作用。少