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ACTION OF NITRIC OXIDE ON THE INTERNAL URETHRAL SPHINCTER FUNCTION

一氧化氮对尿道内括约肌功能的作用

基本信息

批准号：
05671297
负责人：
NISHIZAWA Osamu
金额：
$ 1.15万
依托单位：
AKITA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671297/
关键词：
Nitric oxide Internal urethral sphincter Urethral relaxation 膀胱尿道 Nitric oxide

项目摘要

The present study was performed to investigate action of nitric oxide on the internal urethral sphincter function. Female rats were anesthetzed with urethane. The bladder neck was ligated in order to measure independently intravesical pressure and internal urethral pressure. Under condition of abolishment of the urethral striated muscle activity by the intravenous administration of pancronium bromide, the bladder and urethral pressure were continuosly recorded during rhythmic isovolumetric bladder contractions before and after intraarterial administration of the following drugs. Used drugs were L-arginine from which nitric oxide is synthetized, L-NMMA,L-NAME which inhibit the synthesis of nitric oxide from L-arginine, and methylene blue which inhibits the activation of soluble guanylyl cyclase. Before the administration of each drug, rhythmic bladder contractions with concomitant urethral relaxations were occurred. Administration of L-arginine produced a decrese in urethral pressure. A … More dministration of L-NMMA,L-NAME and methylene blue produced an inhibition of the internal urethral sphincter relaxation during rhythmic bladder contractions. The nitric oxide/cyclic GMP pathway seems to take an important role for the internal urethral sphincter relaxation.Decerebrate dogs were used to examine the effect of L-arginine and methylene blue on the isovolumetric bladder contractions in response to bladder fillig and electrical stimulation of pontine micturition center. In the isovolumetric bladder contractions in response to bladder filling, L-arginine produced an increase in bladder capacity and a decrease in bladder contraction pressure. Methylene blue produced an inhibition of effect of L-arginine. In the isovolumetric bladder contractions in response to electrical stimulation of pontine micturition center, methylene blue produced an increase in the bladder contraction pressure and duration of bladder contraction. The nitric oxide/cyclic GMP pathway seems to take certain role for bladder activity. Less

本研究旨在探讨一氧化氮对内括约肌功能的影响。雌性大鼠用乌拉坦麻醉。结扎膀胱颈，独立测量膀胱内压和尿路内压。在静脉注射潘克罗溴铵消除尿道纹肌肉活动的情况下，连续记录动脉内给药前后节律性等容膀胱收缩时的膀胱压和尿道压。常用药物有：合成一氧化氮的L-精氨酸，抑制L-精氨酸合成一氧化氮的L-NMMA，L-NAME，抑制可溶性鸟苷酸环化酶活性的亚甲蓝。在服用每种药物之前，都会发生有节律性的膀胱收缩和伴随的尿路松弛。应用L-精氨酸可降低尿路压力。A…在节律性膀胱收缩中，L、L和亚甲蓝的剂量越大，对内括约肌松弛的抑制作用越强。NO/cGMP通路可能在内括约肌松弛中起重要作用。本实验在去脑犬模型上观察了L精氨酸和亚甲蓝对膀胱充盈和电刺激桥脑排尿中枢所致等容膀胱收缩的影响。在膀胱充盈所致的等容膀胱收缩中，L-精氨酸使膀胱容量增加，膀胱收缩压降低。亚甲蓝对L-精氨酸有抑制作用。在电刺激桥脑排尿中枢引起的等容膀胱收缩中，亚甲蓝使膀胱收缩压力增加，收缩持续时间延长。一氧化氮/环GMP途径似乎在膀胱活动中起一定作用。较少