权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Gene expression as possible biomarker in bladder urothelium of patients with ulcerative interstitial cystitis

溃疡性间质性膀胱炎患者膀胱尿路上皮的基因表达作为可能的生物标志物

基本信息

批准号：
20591876
负责人：
NISHIZAWA Osamu
金额：
$ 3.08万
依托单位：
Shinshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591876/
关键词：
間質性膀胱炎遺伝子発現バイオマーカー

项目摘要

We investigated the genes responsible for ulcerative interstitial cystitis by DNA microarray analysis and quantitative real-time polymerase chain reaction. Bladder urothelial tissues were taken from a site apart from the ulcerative lesion in 9 patients with ulcerative interstitial cystitis and from a normal-looking area in 9 controls, including 7 with bladder carcinoma and 2 with benign prostatic hyperplasia. Total RNA was extracted from bladder samples and gene expression was compared between these 2 groups using Whole Human Genome DNA microarray 44K (Agilent Technologies, Santa Clara, California). Microarray data were analyzed by GeneSpring GX software and Ingenuity Pathway Analysis. Chosen genes were confirmed for altered transcription by quantitative real-time polymerase chain reaction. We identified 564 probes that were significantly expressed in mRNA more than 4-fold vs those in controls using volcano plot analysis (p<0.001). Further network Ingenuity Pathway Analysis of these genes showed the top 3 functions, including 1) cell-to-cell signaling and interaction, and hematological system development and function, 2) inflammatory disease and 3) cellular development. Quantitative real-time polymerase chain reaction confirmed increased mRNA expression of several genes in the bladder samples of patients with ulcerative interstitial cystitis, including CXCR3 binding chemokines (CXCL9, 10 and 11) and tumor necrosis factor ligand superfamily member 14. Our study using DNA microarray analysis followed by quantitative real-time polymerase chain reaction reveals over expression of genes related to immune and inflammatory responses, including T-helper type 1 related chemokines, and cytokines such as CXCR3 binding chemokines and tumor necrosis factor ligand superfamily member 14. These genes may be potential interstitial cystitis biomarkers.

我们通过DNA芯片分析和实时定量聚合酶链反应研究了溃疡性间质性膀胱炎的致病基因。9例溃疡性间质性膀胱炎患者的膀胱尿路上皮组织取自溃疡病变以外的部位，9例对照患者的膀胱尿路上皮组织取自外观正常的部位，其中7例膀胱癌患者和2例良性前列腺增生患者。从膀胱样本中提取总RNA，并使用全人类基因组DNA芯片44K （Agilent Technologies, Santa Clara, California）比较两组之间的基因表达。芯片数据采用genesspring GX软件和Ingenuity Pathway Analysis进行分析。选择的基因通过定量实时聚合酶链反应证实转录改变。通过火山图分析，我们发现564个探针的mRNA显著表达量是对照组的4倍以上（p<0.001）。进一步对这些基因的网络匠心途径分析显示了前3个功能，包括1)细胞间信号传导和相互作用，血液系统发育和功能，2)炎症疾病和3)细胞发育。实时定量聚合酶链反应证实溃疡性间质性膀胱炎患者膀胱样本中多个基因mRNA表达增加，包括CXCR3结合趋化因子（CXCL9、10和11）和肿瘤坏死因子配体超家族成员14。我们的研究使用DNA微阵列分析和定量实时聚合酶链反应，揭示了免疫和炎症反应相关基因的过度表达，包括辅助t型1相关趋化因子和细胞因子，如CXCR3结合趋化因子和肿瘤坏死因子配体超家族成员14。这些基因可能是潜在的间质性膀胱炎生物标志物。