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The research for the therapy of myoma uteri and endometriosis using Gn-RH receptor

Gn-RH受体治疗子宫肌瘤及子宫内膜异位症的研究

基本信息

批准号：
05671381
负责人：
ISHIMARU Tadayuki
金额：
$ 1.41万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671381/
关键词：
Gn-RH Receptor Autoradiography Myoma Uteri Endometriosis GnRH analogue 受容体 -

项目摘要

We exam ined whether gonadotrop in releasing horm one receptor (Gn-RH receptor) existed or did not exist in normal myometrium and myoma nodule using the auto radiography method. We used ^<125>I-Buserelin as Gn-RH analogue and used pituitary anterior lobe and hippocampus of rat as control samples. We recognized there were specific bindings of ^<125>I-Buserelin to pituitary anterior lobe and hippocampus of rat. However, the binding activities of ^<125>I-Buserelin to normal myometrium and myoma nodule were variable and we could not demonstrate their specific binding sites of ^<125>I-Buscrelin in those tissues. Although some incubation conditions, that were preincubation time and incubation temperature et al. were changed, we could not find the specific binding sites of ^<125>I-Buserelin in normal myometrium and myoma nodule. In conclusion, we could not demonstrate the existence of Gn-RH receptor in myometrium and myoma nodule.In the next stage, using cell culture system we examined whether Gn-RHa had the direct effects to uterine myoma. 1x10^5 cells/dish of myoma and normal myometrium were cultured in the medium which contained several concentrations of Gn-RHa, and the number of cells/dish and the volume of protein/dish were analyzed every 2 days for 2 weeks. There were no significant differences in the number of cells/dish and the volume of protein/dish between the Gn-RHa contained group and the control group. Therefore, we guessed that Gn-RHa had no direct effect to the proliferative ability of myoma and normal myometrial cells.In conclusion, it was guessed that the effect of Gn-RHa that reduced the volume of uterine myoma was not a direct effect of Gn-RHa through Gn-RH receptor but an indirect effect which was, for example, due to low estrogenic environment.

采用自体X线摄影方法检查正常子宫肌层和肌瘤结节中是否存在释放激素一受体（Gn-RH受体）的促性腺激素。我们使用^ 125 I-布舍瑞林作为Gn-RH类似物并使用大鼠垂体前叶和海马作为对照样品。我们认识到125 I-布舍瑞林与大鼠垂体前叶和海马有特异性结合。然而， 125 I-布丝瑞林与正常子宫肌层和肌瘤结节的结合活性是可变的，并且我们无法证明它们在这些组织中的 125 I-布丝瑞林的特异性结合位点。尽管有一些孵化条件，即预孵化时间和孵化温度等。改变后，我们在正常子宫肌层和肌瘤结节中找不到^ 125 I-Buserelin的特异性结合位点。总之，我们无法证明Gn-RH受体在子宫肌层和肌瘤结节中的存在。在下一阶段，我们利用细胞培养系统检测Gn-RHa是否对子宫肌瘤有直接作用。将1x10^5个细胞/皿的肌瘤和正常子宫肌层培养在含有多个浓度的Gn-RHa的培养基中，每2天分析细胞/皿的数量和蛋白/皿的体积，持续2周。含有Gn-RHa的组与对照组之间，细胞/培养皿的数量和蛋白质/培养皿的体积没有显着差异。因此，我们推测Gn-RHa对肌瘤和正常子宫肌层细胞的增殖能力没有直接影响。综上所述，推测Gn-RHa缩小子宫肌瘤体积的作用不是Gn-RHa通过Gn-RH受体的直接作用，而是间接作用，例如由于低雌激素环境所致。