Involbement of adhesion molecules in osteoclast formation

粘附分子参与破骨细胞形成

• 批准号: 05671540
• 负责人: MORITA Ikuo
• 金额: $ 1.34万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671540/
• 关键词: osteoclasts; cell-cell fusion; bone metaboilsm; stromal cells; mannose residues; ストローマ細胞; CD11; CD18; TCAM-1; 接着分子

Osteoclast, the bone resorbing cell, is formed from hematopoietic precursors via cell-cell fusion. To evaluate the possibility that under certain specific conditions mannose residues may be expressed on the preosteoclast cell surface, we examined the action of some compound which bind terminal mannose specifically, on the farmation of osteoclast induced in the coculture of mouse spleen cells with mouse stromal cell, TMS,a process in which cell-cell fusion is involved.Osteoclast formation was inhibited by treatment of this coculture system with some terminal mannose-binding drug at the fusion evednt. Whith an interactive lazer cytometer ACAS570, fluorescein isothiocyanate labeled drug, which binds mannose residue specifically, was observed to bind osteolast progenitors at thefusion process between mononuclear preosteoclasts.These results suggest that mannose residues were expressed on outer membranes of monocytes under pathophysiological conditions and that they were involved in the osteoclast formation via cellular membrane fusiom events.

破骨细胞是由造血前体细胞通过细胞-细胞融合形成的骨吸收细胞。为了评估在某些特定条件下甘露糖残基可能在破骨细胞前体细胞表面表达的可能性，我们检测了某些特异性结合末端甘露糖的化合物对小鼠脾细胞与小鼠基质细胞TMS共培养中诱导的破骨细胞培养的作用，一种涉及细胞与细胞融合的过程。在融合发生时，用某些末端甘露糖结合药物处理该共培养系统可抑制破骨细胞的形成。用ACAS 570激光流式细胞仪观察到特异性结合甘露糖残基的异硫氰酸荧光素标记药物在单核前破骨细胞融合过程中与破骨祖细胞结合，提示在病理生理条件下，甘露糖残基表达于单核细胞外膜，并通过细胞膜融合事件参与破骨细胞的形成。

项目成果

T.Kurachi,I.Morita: "Expression of outer membranes of mannose residues,which are involved in osteoclast formation via cellular fusion" J.Biol.Chem.269. 17572-17576 (1994)

T.Kurachi，I.Morita：“甘露糖残基外膜的表达，通过细胞融合参与破骨细胞的形成”J.Biol.Chem.269。

K.Sakaguchi,I.Morita: "Eicosapentaenoic acid inhibits bone loss due to avariectomy in rats" PG.LT and EFA. 51. 51-55 (1994)

K.Sakaguchi，I.Morita：“二十碳五烯酸抑制大鼠因卵巢切除术引起的骨质流失”PG.LT 和 EFA。

T.Kurachi, I.Morita et al.: "Expression on outher membranes of mannose residues which are involved in osteoclast formation via cell fusion events" J.Biol.Chem. 269. 17572-17576 (1994)

T.Kurachi、I.Morita 等人：“通过细胞融合事件参与破骨细胞形成的甘露糖残基外膜上的表达”J.Biol.Chem。

K.Sakaguchi,I.Morita: "Eicosapentaenoic acid inhibits bone loss due to avariectomy in rats" PG.LT.EFA.(in press).

K.Sakaguchi，I.Morita：“二十碳五烯酸抑制大鼠因卵巢切除术引起的骨质流失”PG.LT.EFA.（印刷中）。

N.Suda,I.Morita etc.: "Partification of oxidative stress in the presence of osteoclasts differentiation" Biochim.Biophys.Acta. 1151. 318-323 (1993)

N.Suda，I.Morita 等：“破骨细胞分化中氧化应激的部分”Biochim.Biophys.Acta。