Involbement of adhesion molecules in osteoclast formation

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Osteoclast, the bone resorbing cell, is formed from hematopoietic precursors via cell-cell fusion. To evaluate the possibility that under certain specific conditions mannose residues may be expressed on the preosteoclast cell surface, we examined the action of some compound which bind terminal mannose specifically, on the farmation of osteoclast induced in the coculture of mouse spleen cells with mouse stromal cell, TMS,a process in which cell-cell fusion is involved.Osteoclast formation was inhibited by treatment of this coculture system with some terminal mannose-binding drug at the fusion evednt. Whith an interactive lazer cytometer ACAS570, fluorescein isothiocyanate labeled drug, which binds mannose residue specifically, was observed to bind osteolast progenitors at thefusion process between mononuclear preosteoclasts.These results suggest that mannose residues were expressed on outer membranes of monocytes under pathophysiological conditions and that they were involved in the osteoclast formation via cellular membrane fusiom events.