Development of tissue engineering for three-dimensional tissue regeneration

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To establish the angioplasty method using the novel printing technique, we developed a new substrate prepared by coating glass with polyethylene glycol to create a non-adhesive surface and subsequent photo-lithography to finely tune the adhesive property for efficient cell transfer. MicroCT analysis showed rapid and effective bone formation by the cell-equipped amniotic membrane. The cell-printing and transfer technology used to create the cell-equipped amniotic membrane was beneficial for the cell delivery system. Moreover, using scar model we developed the cell-equipped amnion membrane was examined.