Involvement of PPAR γ in senescence-induced osteoporosis
PPAR γ 参与衰老引起的骨质疏松症
基本信息
- 批准号:12671799
- 负责人:
- 金额:$ 2.37万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Senescence-induced osteoporosis is thought to be a low turnover type and to be due to the shift from osteoblastogenesis to adipogenesis. In this study, we investigated the effects of some drugs affected bone metabolism on adipose differentiation and the effects of adipogenesis on osteoclastogenesis and osteoblastogenesis. We used mouse preosteoblast cell lines established in our laboratory and human mesenchymal stem cells isolated from bone marrow. The PPARγ ligands such as 15-deoxy.-Δ 12,14-PGJ2 and trogritazone stimulated adipogenesis, but IL-11 and vitaminK2 suppressed the adipogenesis. The adipocyte differentiation caused to decrease the osteoclast formation-supporting activity, but did not affect osteoblastogenesis. The similar results were obtained from in vivo study. When human mesenchymal cells with BMP-2 and vitamin K2 inoculated to Matrigel and transplanted subcutaneously to nude mice, the cells was differentiated to osteoblasts and adipocytes by BMP-2. The effect of vitamin K2 on osteoblastogenesis failed to be observed in spite of the suppression of adipogenesis.These data indicate that the suppression of adipogenesis is not benefit for the protect of senescence-induced osteoporosis.
衰老引起的骨质疏松症被认为是一种低转换类型,是由于成骨细胞向脂肪细胞转化所致。在这项研究中,我们研究了一些影响骨代谢的药物对脂肪分化的影响,以及脂肪形成对破骨细胞和成骨细胞形成的影响。我们使用在我们实验室建立的小鼠前成骨细胞系和从骨髓中分离的人间充质干细胞。过氧化物酶体增殖物激活受体γ配体,如15-脱氧。Δ 12,14-PGJ 2和曲格列酮促进脂肪形成,而IL-11和维生素K2抑制脂肪形成。脂肪细胞分化导致破骨细胞形成支持活性降低,但不影响成骨细胞的生成。体内实验也得到了类似的结果。将含有BMP-2和维生素K2的人间充质细胞接种到Matrigel上并皮下移植到裸小鼠体内,细胞在BMP-2的作用下分化为成骨细胞和脂肪细胞。维生素K_2对成骨细胞的作用不明显,但对脂肪细胞的作用明显抑制,提示抑制脂肪细胞的形成对预防衰老性骨质疏松症没有益处。
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Gao Y: "Expression of adhesion molecules LEA-1 and ICAM-lon osteoclast precursors during osteoclast differentiation and involvement of estrogen deficiency"CLIMACTERIC. 3. 278-287 (2000)
高Y:“破骨细胞分化过程中粘附分子LEA-1和ICAM-lon破骨细胞前体的表达以及雌激素缺乏的参与”CLIMACTERIC。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakaguchi K: "Relationship between the ability to support differentiation of osteoclastlike cells and adipogenesis in murine stromal cells derived from bone marrow"Prostaglandin Leukotriene & Essential Fatty Acids. 62.5. 319-327 (2000)
坂口 K:“骨髓来源的小鼠基质细胞支持破骨细胞样细胞分化的能力与脂肪形成之间的关系”前列腺素白三烯
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Palacious VG: "Expression of adipogenesis markers in a murine stromal cell line treated with 15-deoxy Δ12,14-prostaglandin J2, intereleukin-11,9-cis retinoic acid and vitamin K2"Prostaglandin Leukotriene & Essential Fatty Acids. 65.4. 215-221 (2001)
Palacious VG:“用 15-脱氧 Δ12,14-前列腺素 J2、白介素-11,9-顺式视黄酸和维生素 K2 处理的小鼠基质细胞系中脂肪生成标记物的表达”前列腺素白三烯和必需脂肪酸 215-。 221(2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakaguchi K: "Relationship between the ability to support differentiation of osteoclast-like cellsand adipogenesis in murine stromal cells derived from bone marrow"Prostaglandins Leukotrienes & Essential Fatty Acids. 62・5. 319-327 (2000)
Sakaguchi K:“支持破骨细胞样细胞分化的能力与源自骨髓的小鼠基质细胞中的脂肪生成之间的关系”前列腺素白三烯和必需脂肪酸 62・5(2000)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Palacious VG: "Expression of adipogenesis markers in a murine stromal cell line treated with 15-deoxy- Δ 12, 14-prostaglanidn J2, interleukin-11, 9-cis retinoic acid and vitamin K2"Prostaglanidn Leukotriene & Essential Fatty Acids. 65・4. 215-224 (2001)
Palacious VG:“用15-脱氧-Δ12、14-前列腺素J2、白细胞介素-11、9-顺式视黄酸和维生素K2处理的小鼠基质细胞系中脂肪生成标记物的表达”前列腺素白三烯和必需脂肪酸65·。 4. 215-224(2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MORITA Ikuo其他文献
MORITA Ikuo的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MORITA Ikuo', 18)}}的其他基金
Development of novel therapies for myopia by printing technology
利用印刷技术开发近视新疗法
- 批准号:
25670728 - 财政年份:2013
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
A novel periodontal tissue regeneration method using a printing technology
一种利用打印技术的牙周组织再生新方法
- 批准号:
24390442 - 财政年份:2012
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of tissue engineering for three-dimensional tissue regeneration
三维组织再生组织工程的发展
- 批准号:
22659354 - 财政年份:2010
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Mechanism for maintenance of homeostasis of several functions by connexin 43
连接蛋白 43 维持多种功能稳态的机制
- 批准号:
20390470 - 财政年份:2008
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of pathology based on four-dimensional analysis of intracellular communication through gap junction and their development for therapy
基于间隙连接细胞内通讯四维分析的病理学分析及其治疗发展
- 批准号:
17209058 - 财政年份:2005
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Regulation of angiogenesis by PEDF, anti-angiogenic factor, for controlling Oral Diseases
通过 PEDF(抗血管生成因子)调节血管生成,以控制口腔疾病
- 批准号:
15390558 - 财政年份:2003
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Identification of inhibitor of osteoclastogenesis
破骨细胞生成抑制剂的鉴定
- 批准号:
10671734 - 财政年份:1998
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Physiological role of prostaglandin H2 synthase-2 (COX-2) in bone metabolism
前列腺素 H2 合酶 2 (COX-2) 在骨代谢中的生理作用
- 批准号:
08672119 - 财政年份:1996
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Involbement of adhesion molecules in osteoclast formation
粘附分子参与破骨细胞形成
- 批准号:
05671540 - 财政年份:1993
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Isolation of osteoclast-forming factor produced by stromal cells and mechanism of osteoclast differentiation
基质细胞产生的破骨细胞形成因子的分离及破骨细胞分化机制
- 批准号:
03670864 - 财政年份:1991
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似国自然基金
支链氨基酸代谢紊乱调控“Adipocytes - Macrophages Crosstalk”诱发2型糖尿病脂肪组织功能和结构障碍的作用及机制
- 批准号:81970721
- 批准年份:2019
- 资助金额:55.0 万元
- 项目类别:面上项目
相似海外基金
New development of cellular regeneration therapy in jaw bone using stem cells derived from adipocytes jaw bone
利用颌骨脂肪细胞来源的干细胞进行颌骨细胞再生治疗的新进展
- 批准号:
23K16058 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
A novel mechanism of insulin resistance mediated by uric acid metabolism in adipocytes
脂肪细胞尿酸代谢介导胰岛素抵抗的新机制
- 批准号:
23K10969 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Hypertrophic adipocytes as biophysical mediators of breast cancer progression
肥大脂肪细胞作为乳腺癌进展的生物物理介质
- 批准号:
10751284 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Development of adipocytes for gene therapy that avoids cellular stress due to overexpression of therapeutic proteins
开发用于基因治疗的脂肪细胞,避免因治疗蛋白过度表达而造成的细胞应激
- 批准号:
23H03065 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional analysis of bitter taste receptors in adipocytes and hepatocytes
脂肪细胞和肝细胞中苦味受体的功能分析
- 批准号:
23K05107 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of mechanisms for conversion of adipocytes to cancer-associated fibroblasts in osteosarcoma microenvironment
阐明骨肉瘤微环境中脂肪细胞转化为癌症相关成纤维细胞的机制
- 批准号:
23K19518 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
Study on UCP-1 independent metabolic regulation by brown adipocytes
棕色脂肪细胞对UCP-1独立代谢调节的研究
- 批准号:
23K18303 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
NKA/CD36 signaling in adipocytes promotes oxidative stress and drives chronic inflammation in atherosclerosis
脂肪细胞中的 NKA/CD36 信号传导促进氧化应激并驱动动脉粥样硬化的慢性炎症
- 批准号:
10655793 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
The mechanisms of the signal transduction from brown adipocytes to afferent neurons and its significance.
棕色脂肪细胞向传入神经元的信号转导机制及其意义。
- 批准号:
23K05594 - 财政年份:2023
- 资助金额:
$ 2.37万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Characterizing breast cancer invasion and proliferation when co-aggregated with adipocytes in multicellular spheroids created with a custom bioreactor to augment cell-cell connectivity.
当与多细胞球体中的脂肪细胞共聚集时,表征乳腺癌的侵袭和增殖,该多细胞球体是用定制生物反应器创建的,以增强细胞间的连接。
- 批准号:
10334113 - 财政年份:2022
- 资助金额:
$ 2.37万 - 项目类别: