Immunohistochemical Study on Gene Expression of Oral Precancerous Lesions with Reference to Malignant Transformation

口腔癌前病变与恶变相关基因表达的免疫组织化学研究

• 批准号: 05671677
• 负责人: SHIBA Ryosuke
• 金额: $ 1.34万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671677/
• 关键词: Fos protein; Jun protein; Precancerous lesions; Suquamous cell carcimoma; Malignant transformation; Oral mucosa; lmmunohistochemistry; Antigen retrieval methods; Fos蛋白質; Jun蛋白質

Histochemical observation of c-Fos and c-Jun was carried out by using their polyclonal antibodies in 49 patients with various kinds of oral lesions such as precancerous lesions, well- and moderately-differentiated squamous cell carcinomas and non-tumorous epithelial tissues with reference to these gene expressions and malignant transformation. For elevation of detectability of these gene proteins, the sections were treated by an autoclave or a microwave, that is an antigen retrieval method, and the following results were obtained :1.No positive cells for c-Fos or c-Jun could be detected without the antigen retrieval methods, however, c-Fos-or c-Jun-positive cells were detected in many kinds of tissues after treatment with this methods. More number of positive cells and more intensity of reaction were showed in the sections treated by the autoclave than in those treated by the microwave. However, there was no significant difference about distribution of c-Fos-or c-Jun-positive cells between the tissues treated by autoclave and microwave.2.In the non-tumorous tissues, both of c-Fos-and c-Jun-positive cells distributed in the granular layr adjucent to the cornified layr. In the precancerous and cancerous tissues, these gene proteins did not always located in the same layr of epithelial tissue.3.Intensity of reaction and number of positive cells for c-Fos and c-Jun were compared among many kinds of epithelial benign and malignant tissues, and following orders were observed : non-tumorous epithelial tissues>precancerous lesions>well-differentiated squamous cell carcinomas>moderately-differentiated squamous cell carcinomas. Especially, the expression of c-Jun had these tendency more typically. From the above results, it was considered that the expression of c-Fos and c-Jun was inhibited, reflecting to malignant transformation as well as to malignancy.

应用c-Fos和c-Jun的多克隆抗体，对49例口腔癌前病变、高、中分化鳞癌及非肿瘤性上皮组织中c-Fos和c-Jun的表达及癌变情况进行了组织化学观察。为了提高这些基因蛋白的可检测性，将切片用高压灭菌或微波处理，即抗原修复法，得到以下结果：1.未经抗原修复法处理的组织中不能检测到c-Fos或c-Jun阳性细胞，但经抗原修复法处理后，在多种组织中检测到c-Fos或c-Jun阳性细胞。高压灭菌处理组的阳性细胞数和反应强度均高于微波处理组。2.在非肿瘤组织中，c-Fos和c-Jun阳性细胞均分布于与皮质层相邻的颗粒层。在癌前病变和癌组织中，这些基因蛋白并不一定位于同一层上皮组织中。3.比较了多种上皮良恶性组织中c-Fos和c-Jun的反应强度和阳性细胞数，其顺序为：非肿瘤上皮组织>癌前病变>高分化鳞癌>中分化鳞癌。尤其是c-Jun的表达更具有典型性。从以上结果可以认为，c-Fos和c-Jun的表达受到抑制，反映了恶性转化以及恶性肿瘤。