Osteogenesis by Conjugation of Bone Morphogenetic Protein Derived from Human Dentin Matrix and Multipolar Hydroxyapatite.

通过人牙本质基质衍生的骨形态发生蛋白和多极羟基磷灰石的缀合来成骨。

• 批准号: 01571098
• 负责人: SHIBA Ryosuke
• 金额: $ 1.34万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571098/
• 关键词: Human Dentin Matrix; Chondrogenesis; Tissue Culture; Bone Morphogenetic Protein; Hydroxyapatite; ヒト象芽質基質; 骨形成; アテロコラ-ゲン; 未分化間葉組織; 骨形成性蛋白

1. In vitro chondrogenesis with human tooth matrix gelatin (hーTMG) :(1) H-TMG was prepared from human teeth by the method of Urist et al. (2) Minced femoral muscle from rat fetus was incubated on the h-TMG. (3) Histological observations revealed that at first immature fibroblastic cells migrated from muscle tissue and then altered to chondrocytes in contact with h-TMG. Cartilage was formed on the h-TMG, but no bone was formed.2. In vivo osteogenesis with h-TMG or rat tooth matrix gelatin (r-TMG) :(1) H-TMG or r-TMG was inoculated subcutaneously in rats. (2) Bone formation was detected in the rats which r-TMG was inoculated to, but not in ones which h-TMG was inoculated to.3. In vivo osteogenesis with partial purified bone morphogenetic protein (PPBMP) from human teeth :(1) PPBMP was prepared from hーTMG by extraction with 4M GuHCl and gel filtration (15,000 - 40,000 MW). (2) When mixture of PPBMP and bovine athelocollagen was inoculated subcutaneously in rats, bone formation was detected. However, when mixture of PPBMP and hydroxyapatite (HA) grains was inoculated,o bone formation could be detected.4. Conclusion : (1) h-TMG, as well as r-TMG, contains special protein which stimulates immature mesenchimal cells to differentiate to osteoblasts and to form bone tissue. (2) An ideal artificial bone, which is able to induce bone tissue, might be botained, if HA is so united with PPBMP that the BMP is delivered gradually.

1.人牙基质明胶(h-ー-TMG)的体外软骨形成：(1)用Urist等人的方法从人牙中制备H-TMG。(2)取胎鼠股骨肌碎块与h-TMG孵育。(3)组织学观察显示，未成熟的成纤维细胞首先从肌肉组织中迁移出来，然后转化为软骨细胞，与h-TMG接触。结论：1.h-TMG表面有软骨形成，但无骨形成。H-TMG或大鼠牙基质明胶(r-TMG)体内成骨：(1)大鼠皮下接种h-TMG或r-TMG。(2)接种r-TMG的大鼠骨形成明显，而接种h-TMG的大鼠未见骨形成。人牙部分纯化的骨形态发生蛋白的体内成骨作用：(1)h-ー骨形态发生蛋白经4M盐酸提取和凝胶过滤(15,000-40,000 mW)制备成骨形成蛋白。(2)大鼠皮下接种PPBMP和牛膝胶原后，可观察到成骨现象。然而，当PPBMP和羟基磷灰石(HA)颗粒混合接种时，可以检测到骨形成。结论：(1)h-TMG和r-TMG均含有刺激未成熟间充质细胞向成骨细胞分化并形成骨组织的特殊蛋白。(2)将HA与PPBMP复合，使BMP逐步输送，可获得理想的骨组织诱导型人工骨。

项目成果

Ihoki M. and Shiba R.: "Chondrogenesis following induction of alkaline phosphatase activity in fetal rat muscle tissue by human and rat demineralized tooth matrix gelatin." Arch oral Biol.

Ihoki M. 和 Shiba R.：“人类和大鼠脱矿质牙基质明胶在胎鼠肌肉组织中诱导碱性磷酸酶活性后的软骨形成。”

Ihoki M.and Shiba R.: "Chondrogenesis following induction of alkaline phosphatase activity in foetal rat muscle tissue by human and rat demineralized tooth matrix gelatin." Arch oral Biol.

Ihoki M. 和 Shiba R.：“人和大鼠脱矿牙基质明胶诱导胎鼠肌肉组织中碱性磷酸酶活性后的软骨形成。”

Ihoki,M.,Shiba,R.: "Chondrogenesis following induction of alkaline phosphatase activity in foetal rat muscle tissus by human and rat demineralized tooth matrix gelatin" Arch oral Biol.

Ihoki,M.,Shiba,R.：“人和大鼠脱矿质牙基质明胶诱导胎鼠肌肉组织中碱性磷酸酶活性后的软骨形成”Archoral Biol。