Immunohistochemical study on alternative expressions of various transcription factors in carcinogenesis of oral mucosa

口腔黏膜癌变过程中多种转录因子选择性表达的免疫组化研究

• 批准号: 09672057
• 负责人: SHIBA Ryosuke
• 金额: $ 2.05万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672057/
• 关键词: precancerous lesion; oral mucosa; carcinogenesis; c-Fos; c-Jun; immunohistochemistry; cーfos; cーjun; c-fos; c-jun

It has been well known that malignant transformation of a cell is concerned in changes of transcription factors. In this work, we aimed at the changes of c-fos and c-jun genes.Several kinds of oral mucosal lesion (cancer, precancer and normal mucosa) obtained from patients, which have been stocked in our department, were fixed in formalin and embedded in paraffin. The paraffin sections were immunohistochemically stained for c-Fos and c-Jun proteins.As a result, expression of c-Fos and c-Jun was highest in frequency and intensity in normal epithelial cells and lowest in squamous cell carcinomas. Several precancerous lesions showed various degree of frequency and intensity case by case. Among squamous cell carcinomas, poorly-differentiated ones were weaker than well-differentiated ones.C-fos and c-jun genes have been reported to be expressed in connection with keratinization or differentiation of the epithelial cells. It is considered that decreased expression of these genes means disturbance of cell differentiation and start of malignant transformation.Considering the above results, the lesions of leucoplakia and lichen planus showing decreased expression of c-Fos and c-Jun is considered to have a tendency to transform into malignancy.

众所周知，细胞的恶性转化与转录因子的变化有关。本研究以c-fos和c-jun基因的变化为研究对象，对我科保存的几种口腔粘膜病变（癌、癌前病变和正常粘膜）标本进行福尔马林固定和石蜡包埋。结果表明，c-Fos和c-Jun蛋白在正常上皮细胞中表达频率和强度最高，在鳞癌中表达最低。几种癌前病变的出现频率和强度各不相同。在鳞状细胞癌中，低分化者比高分化者弱，C-fos和c-jun基因的表达与上皮细胞的角化或分化有关。认为这些基因表达的降低意味着细胞分化的障碍和恶性转化的开始。考虑到上述结果，认为表现出c-Fos和c-Jun表达降低的白斑和扁平苔藓病变具有向恶性转化的趋势。

项目成果

TERAYAMA,R.: "Differential expression of Fos protein after transection of the rat infraorbital nerve in the trigeminal nucleus caudalis." BRAIN RESEARCH. 768. 135-146 (1997)

TERAYAMA,R.：“横断大鼠三叉神经尾核中眶下神经后 Fos 蛋白的差异表达。”