Changes in the function of neutrophils from diabetic rats

本项目的目的是研究花生四烯酸代谢变化与链脲佐菌素诱导的糖尿病状态下大鼠中性粒细胞功能受损之间的可能联系。结果发现：1）糖尿病大鼠中性粒细胞磷脂中花生四烯酸的比例明显降低; 2）与正常大鼠相比，A23187刺激的糖尿病大鼠中性粒细胞中游离花生四烯酸LTB_4的产生减少; 3）A23187刺激的糖尿病大鼠中性粒细胞聚集明显受到抑制。然而，花生四烯酸诱导的中性粒细胞从糖尿病和正常大鼠聚集到相似的程度。本研究表明，糖尿病相关的花生四烯酸代谢的变化可能在调节中性粒细胞聚集的关键作用。

The aim of this project was to examine a possible link between changes in the metabolism of arachidonic acid and impairment of the function of rats neutrophils in the state of diabetes induced by streptozotocin. We found several results as follows ;1) Profound decreases in the proportion of arachidonic acid of phospholipids were found in neutrophils from diabetic rats.2) There were decreases in the production of free arachidonic acid LTB4 in A23187-stimulated from diabetic rats as compared to those from normal rats.3) Aggregation of neutrophils provoked by A23187 was significantly suppressed in neutrophils from diabetic rats. However, arachidonic acid induced aggregations of neutrophils from diabetic and normal rats to similar extents.The present study suggest that diabetes-associated changes in arachidonic acid metabolism might play a critical role in the modulation of aggregation of neutrophils.