Metabolism of oxidative phospholipids in peroxidized human erythrocytes membranes

过氧化人红细胞膜中氧化磷脂的代谢

• 批准号: 07672376
• 负责人: NAKAGAWA Yasuhito
• 金额: $ 1.34万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672376/
• 关键词: oxidative stress; lipid peroxide; oxidative phospholipid; aldehydic phospholipid; ageing; 生成膜; 生体膜

The aim of this project was to examine the metabolism of oxidized phospholipids in biological membranes peroxidized with different kinds of radicals which were alkyl radical produced with AAPH and hydroxy radical with xanthin/xanthin oxidase. CGP hydroperoxide as a primary product of peroxidation significantly formed in RBC membrane peroxidized with AAPH.No consisitent generation of CGP hydroperoxide was evident in XO/XOD/Fe peroxidation, indicating that CGP hydroperoxide is instable in this system. Aldehydic CGP as a secondary product of hydroperoxide decomposition also detected in AAPH-peroxidized RBC membrane. AAPH mainly oxidized CGP to hydroperoxide and aldehydic phospholipids and the production of thiobarbityric acid-reactive substance (TBARS) was relatively low. On the other hand, XO/XOD/Fe peroxidation caused the potential decompositon of phospholipids to eliminate large amounts of TBARS in conrast to generate a trace amounts of oxidized phopholipids. The present study indicates that the process of peroxidation of phospholipids in biomembrane greatly depends on their strucure and the peroxidation condition.The amounts of oxidized phospholipids were also determined in various tissues of young and old rats. Phospholipid hydroperoxides and aldehydic phospholipids in liver, brain heart and kidney of old rats were significantly higher than those of young rats.The present studt demonstrated the productions of oxidazed phospholipids, especially aldehydic phospholipids in biological samples suffered with oxidative stress at the first time.

本课题的目的是研究不同种类的自由基（AAPH产生的烷基自由基和黄嘌呤/黄嘌呤氧化酶产生的羟基自由基）对氧化磷脂在生物膜中的代谢作用。在AAPH过氧化体系中，红细胞膜主要生成CGP过氧化氢，而在XO/XOD/Fe过氧化体系中，CGP过氧化氢不稳定。醛CGP作为氢过氧化物分解的次级产物也在AAPH-过氧化红细胞膜中检测到。AAPH主要将CGP氧化为过氧化氢和磷脂酰胆碱，硫代巴比妥酸反应物（TBARS）的生成量相对较低。另一方面，XO/XOD/Fe过氧化导致磷脂潜在分解，消除对照中大量的TBARS，产生微量的氧化磷脂。本研究表明，生物膜中磷脂的过氧化过程与磷脂的结构和过氧化条件密切相关，并测定了青年和老年大鼠各组织中氧化磷脂的含量。老年大鼠肝、脑、心、肾组织中的磷脂过氧化氢和磷脂酰胆碱含量明显高于青年大鼠，本研究首次证实了氧化应激生物样品中氧化磷脂的产生，特别是磷脂酰胆碱的产生。