Role of PHGPx on the embryogenesis and sperm maturation

为了解PHGPx(Gpx4)基因敲除小鼠胚胎致死机制，建立了受精卵原代培养条件。野生型胚胎培养3d可形成内细胞团(ICM)和PHGPx的显著表达。而在PHGPx-KO胚胎中，ICM的生长受到明显抑制，培养7d后ICM生长消失。我们制备了导入外源PHGPx基因的PHGPx-Hetero小鼠。通过PHGPx基因的导入拯救了KO-PHGPx小鼠，PHGPx基因在精子中的低表达导致男性严重不育。EDS通过睾丸损伤诱导小鼠不育。异种小鼠的睾丸损伤更明显，其PHGPx的表达几乎是野生型小鼠的一半。

Condition of primary culture of fertilized ovum was established to realized the mechanism of embryonic lethal of knockout mouse of PHGPx (GPx4). Inner cell mass (ICM) and prominent expression of PHGPx were formed during 3days culture of wild type of embryo. On the other hand, growth of ICM was significantly suppressed in PHGPx-KO embryo and was disappeared during 7 days culture. We prepared PHGPx-hetero mouse which were introduced exogeous PHGPx gene. KO-PHGPx mouse was rescued by the introduction of PHGPx gene.Low expression of PHGPx in sperm caused severe infertility of male. EDS induced infertility of mouse by the injury of testis. Injury of testis was more prominent in hetero mouse whose expression of PHGPx was almost half as compare to wild type of mouse.