Development of a new platelet aggregometer based on light scattering.

开发基于光散射的新型血小板聚集计。

• 批准号: 05671919
• 负责人: OZAKI Yukio
• 金额: $ 1.28万
• 依托单位: Yamanashi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671919/
• 关键词: Platelets; aggregation; aggregometry; light scatter; light transmission; particle counting; 小凝集塊; 光透過性

A novel meghod has been developed to detect platelet aggregation by means of the Particle counting technique using light scattering. An optical device disigned to focus upon a limited area of platelet-rich plasma measured the intensity of light scattered by particles passing through the area, minimizing multiple light scattering. The use of polystirene spheres of different diameters confirmed that the light scattering intensity increases in proportion to the particle size in a suspension. Platelet activation induced by various agonists resulted in light scattering of higher intensities, which correlated well with the number and size of aggregates as observed under a microscope. These findings confirmed that the intensity of light scattering detected by the new device provides information on the number and size of aggregates in a suspension.The new method was compared with conventional platelet aggregometry using overall light scattering or changes in light transmission (optical density). The new device appeared to be particularly sensitive to small aggregates such as those formed in platelet activation induced by low concentrations of agonists. Furthermore, the new method has an advantage over the conventional aggregometry, in that it allows the aggregate size distribution and the extent of aggregation to be estimated.

提出了一种利用光散射颗粒计数技术检测血小板聚集的新方法。一种光学装置被设计成聚焦在富含血小板的血浆的有限区域上，测量通过该区域的粒子散射的光的强度，最大限度地减少多次光散射。使用不同直径的聚苯乙烯微球证实，光散射强度与悬浮液中的颗粒尺寸成正比增加。不同激动剂诱导的血小板活化导致更高强度的光散射，这与显微镜下观察到的聚集体的数量和大小有很好的相关性。这些发现证实了新设备检测到的光散射强度提供了悬浮液中聚集体的数量和大小的信息。新方法与传统的利用总体光散射或光透过率变化(光密度)的血小板聚集法进行了比较。这种新设备似乎对小聚集体特别敏感，比如低浓度激动剂诱导的血小板活化形成的小聚集体。此外，与传统的集合体测量方法相比，新方法具有优势，因为它允许估计集合体的尺寸分布和聚集程度。

项目成果

Yukio Ozaki,Kaneo Satoh,Yutaka Yatomi,Tetsuya Yamamoto,Yoshiaki Shirasawa,and Shoji Kume: "Detection of platelet aggregates with a particle counting method using ligt scattering" Analytical Biochemistry. 218. 284-294 (1994)

Yukio Ozaki、Kaneo Satoh、Yutaka Yatomi、Tetsuya Yamamoto、Yoshiaki Shirasawa 和 Shoji Kume：“使用光散射的粒子计数方法检测血小板聚集体”分析生物化学。

Ozaki,Y.,Satob,K.,Yatomi,Y.,Yamamoto,T.,Sirasawa,Y.,and Kume,S.: "Detection of platelet aggregates with particle counting method using light scattering" Analyt.Biochem.(in press). (1994)

Ozaki,Y.、Satob,K.、Yatomi,Y.、Yamamoto,T.、Sirasawa,Y. 和 Kume,S.：“使用光散射的粒子计数方法检测血小板聚集体”Analyt.Biochem.（in

尾崎由基男: "散乱光を用いた血小板凝集能の検査" 臨床病理. 96. 46-55 (1993)

Yukio Ozaki：“利用散射光测试血小板聚集能力”《临床病理学》96. 46-55 (1993)。

Yukio Ozaki, Kaneo Satoh, Yutaka Yatomi, Tetsuo Yamamoto, Yoshiaki Shirasawa, and Shoji Kume: "Detection of platelet aggregates with a particle counting method using light scattering." Analytical Biochemistry. 218. 284-294 (1994)

Yukio Ozaki、Kaneo Satoh、Yutaka Yatomi、Tetsuo Yamamoto、Yoshiaki Shirasawa 和 Shoji Kume：“利用光散射的粒子计数方法检测血小板聚集体。”

Yukio Ozaki,Kaneo Satoh,Yutaka Yatomi,Tetsuya,Yamamoto,Yoshiaki Shirasawa,and Shoii Kume: "Detection of platelet aggregates with a particle counting method using ligt scattering" Analytical Biochemistry. 218. 284-294 (1994)

Yukio Ozaki、Kaneo Satoh、Yutaka Yatomi、Tetsuya、Yamamoto、Yoshiaki Shirasawa 和 Shoii Kume：“使用光散射的粒子计数方法检测血小板聚集体”分析生物化学。