Mechanism of synergistic effect on sesame lignans and tocopherols

三个实验证明，芝麻籽及其木脂素产生显著高的α-生育酚浓度，并改善喂食含低α-生育酚饮食的大鼠的维生素E状况。1）在喂食含10（低）、50（正常）、或250（高）mg α-生育酚/kg饮食，含或不含20%芝麻籽8周。血浆和组织中α-生育酚的浓度与饲料中α-生育酚的含量呈平行进行性增加。芝麻种子引起显著增加α-生育酚在血浆和组织中的大鼠喂养任何数量的α-生育酚。然而，仅在低α-生育酚+芝麻籽喂养组中发现γ-生育酚，而在正常或高α-生育酚+芝麻籽喂养组中没有发现。2）在喂养五种饮食的大鼠中检查红细胞溶血、丙酮酸激酶活性和血浆、肝脏和肾脏中过氧化物的变化，作为维生素E缺乏的指标：不含维生素E的对照饮食、低α-生育酚饮食和三种补充有5%、10%和15%芝麻籽的低α-生育酚饮食。这些指数在低α-生育酚饮食中较高，而即使补充5%芝麻籽也能完全抑制这些指数，并导致血浆和肝脏中α-生育酚浓度显著升高。3）两种含有芝麻木脂素的饮食在两个芝麻木脂素喂养组中的结果与在芝麻籽中观察到的结果相当。实验2中的喂食组。这些实验表明，芝麻籽木酚素增强了喂食低α-生育酚饮食的大鼠的维生素E活性，并导致喂食含有芝麻籽或其木酚素的α-生育酚饮食的大鼠的血液和组织中α-生育酚浓度显著增加。

Three experiments demonstrated that sesame seed and its lignans produce significantly high alpha-tocopherol concentrations and improve vitamin E status in rats fed a low alpha-tocopherol containing diet.1) The effect of sesame seed on tocopherol concentrations in the plasma and tissue was studied in rats fed diets containing 10 (low), 50 (normal), or 250 (high) mg alpha-tocopherol/kg diet with or without 20% sesame seed for 8 weeks. The concentrations of alpha-tocopherol in the plasma and tissue showed a progressive increase paralleling the alpha-tocopherol contents in the diets. Sesame seed caused marked increase of alpha-tocopherol in the plasma and tissue of rats fed any amount of alpha-tocopherol. However, gamma-tocopherol was found only in the low alpha-tocopherol + sesame seed-fed group, and not in the normal or high alpha-tocopherol + sesame seed-fed group.2) Changes in red blood cell hemolysis, pyruvate kinase activity, and peroxides in the plasma, liver and kidney, as indices of vitamin E deficiency, were examined in rats fed five diets : a vitamin E-free control diet, a low alpha-tocopherol diet and three low alpha-tocopherol diets supplemented with 5%, 10%, and 15% sesame seed. These indices were high in the low alpha-tocopherol diet, whereas supplementation with even 5% sesame seed suppressed these indices completely and caused significantly higher concentrations of alpha-tocopherol in the plasma and liver.3) Two diets containing sesame lignan (sesaminol or sesamin) and low alpha-tocopherol were tested.Results in both of the sesame lignan-fed groups were comparable to those observed in the sesame seed-fed groups in Experiment 2. These experiments indicate that sesame seed lignans enhance vitamin E activity in rats fed a low alpha-tocopherol diet and cause a marked increase in alpha-tocopherol concentration in the blood and tissue of rats fed an alpha-tocopherol containing diet with sesame seed or its lignans.