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Protective effects of sesame lignans on senescence accelerate brain in rats and mice

芝麻木酚素对大鼠和小鼠大脑衰老的保护作用

基本信息

批准号：
12680143
负责人：
YAMASHITA Kanae
金额：
$ 2.37万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680143/
关键词：
sesame seed sesame lignans α-tocopherol senescence of brain rats SAMP8 TBARS learning test

项目摘要

Recent studies have shown that oxidative stress is important in the pathogenesis of Alzheimer disease and high doses of vitamin E to Alzheimer disease patients improved the disease state. β-Amyloid (Aβ), which is found abundantly in the brains of Alzheimer disease patients, is cytotoxic to neurons and has a central role in the pathogenesis of the disease. There was a paper to report protective effects of α-tocopherol (α-Toc) on Aβ(1-42)-induced learing and memory deficits in rats. Our previous studies showed that sesame seed and its lignans elevated α-Toc concentrations in brain as well as plasma and liver. In this study, we investigated whether the protective effects of sesame seed on senescence accelerated brain were observed in Aβ infused rats and senescence accelerated mouse (SAM)P8. In Experiment 1, we examined α-Toc concentrations in cerebrum, cerebellum, brain stem and hippocampus in brain and TEARS in brain in rats fed diets containing 500 mg/kg α-Toc alone and 50 mg/kg α-Toc with sesame seed. In Experiment 2, learning and memory in radial maze task were examined in rats infused Aβ and fed diets 50 mg/kg α-Toc alone and 50 mg/kg α-Toc with sesame seed. In Experiment 3, passive avoidance task was examined in SAMP8 mice fed the same diets in Exp.2. We obtained the results that sesame seed group showed higher concentrations of α-Toc in all parts of brain examined compared to 500 mg/kg α-Toc group and lower concentration of TEARS in brain, however we could not observe that sesame seed improved learning and memory on senescence accelerated brain in rats and mice.

最近的研究表明，氧化应激在阿尔茨海默病的发病机制中起重要作用，给阿尔茨海默病患者服用高剂量的维生素E可以改善疾病状态。β-淀粉样蛋白(A-β)广泛存在于阿尔茨海默病患者的大脑中，对神经元具有细胞毒性作用，在阿尔茨海默病的发病机制中起着核心作用。有一篇论文报道了生育酚(α-tocoherol，α-ToC)对A-β(1-42)诱导的大鼠学习记忆障碍的保护作用。我们先前的研究表明，芝麻及其木脂素提高了大脑、血浆和肝脏中α-ToC的浓度。在本研究中，我们观察了芝麻籽对A-β灌胃大鼠和衰老加速小鼠P8衰老加速脑的保护作用。在实验1中，我们检测了α-ToC在脑、小脑、脑干和海马区的浓度以及脑内泪液的含量。实验二采用A-β灌胃大鼠，分别饲喂50 mg/kgα-ToC和50 mg/kgα-ToC加芝麻，观察大鼠在径向迷宫中的学习记忆能力。在实验3中，对实验2中饲喂相同饲料的SAMP8小鼠进行被动回避任务的检测。结果表明，芝麻籽组各部位α-ToC含量均高于α-ToC 500 mg/kg组，而脑内泪液浓度低于500 mg/kg组，但未观察到芝麻籽对加速衰老大鼠和小鼠学习记忆的改善作用。