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Anti-Aging Effects of Natural Antioxidants on Senescence Accelerated Mouse (SAM)

天然抗氧化剂对加速衰老小鼠 (SAM) 的抗衰老作用

基本信息

批准号：
63580072
负责人：
YAMASHITA Kanae
金额：
$ 1.54万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63580072/
关键词：
SAM sesame sesaminol catechin Vitamin E peroxidized lipids anti-aging effects

项目摘要

To investigate the effects of antioxidants in foods on the aging of biological function, senescence accelerated mice (SAM) were used throughout this study as a test animal, in addition to Wistar rats. Sesame seed products, sesaminol isolated from sesame oil and catechin isolated from green tea were used as antioxidants and the following results were obtained.1) By long term feeding with the diets containing high levels of crushed sesame seeds, the peroxidation of lipids in the liver and increases of lipofuscin in the testis were effectively depressed in comparison with the control group without crushed sesame seeds.2) The addition of crushed sesame seeds to the diets suppressed the grading score for evaluation of the degree of senecence of SAM, especially the score for glossiness and coarseness of skin hair.3) Peroxidized lipids in the liver and plasma, and hemolysis and pyruvate kinase activity in the plasma were markedly increased in rats fed a tocopherol deficient diet. The addition of sesame products (crushed sesame seeds and oils) which contained -tocopherol exclusively, lowered these characteristics to the same level as the diets containing -tocopherol. However, the addition of -tocopherol to the tocopherol deficient diet depressed the characteristics less than the diet containing -tocopherol.4) The increase of peroxidized lipids in the liver of SAM fed with tocopherol deicient diets was depressed by the addition of sesaminol (0.1%), a potent antioxidative lignan in sesame oil. The dosage of carbon tetra chloride solution to rats also induced lipid peroxidation in its liver, but it was suppressed to an ordinary level by the addition of sesaminol to the solution.5) On the same experiments using tocopherol deficient diets, a slight depression of lipid peroxidation in liver, kidney and plasma was observed by the addition of catechin and its analogues from green tea to the diets.

为了研究食物中抗氧化剂对生物功能衰老的影响，除Wistar大鼠外，本研究还使用衰老加速小鼠（senescence accelerated mice， SAM）作为实验动物。以芝麻产品、芝麻油中分离的芝麻胺和绿茶中分离的儿茶素作为抗氧化剂，得到了以下结果：1)长期饲喂高水平芝麻碎饲料，与不添加芝麻碎饲料的对照组相比，有效抑制了肝脏脂质过氧化和睾丸脂褐素的升高。2)饲粮中添加芝麻碎抑制了SAM衰老程度评分，尤其是皮肤毛发光泽度和粗糙度评分。3)缺乏生育酚的大鼠肝脏和血浆过氧化脂质、血浆溶血和丙酮酸激酶活性均显著升高。添加只含有-生育酚的芝麻产品（芝麻碎和芝麻油），将这些特性降低到与含有-生育酚的日粮相同的水平。然而，在缺乏生育酚的饮食中添加-生育酚比添加-生育酚的饮食对这些特征的抑制要小。4)在缺乏生育酚的饲粮中添加0.1%的芝麻油木质素（sesaminol），可抑制SAM肝脏过氧化脂质的升高。四氯化碳溶液对大鼠肝脏也有诱导脂质过氧化的作用，但通过在溶液中添加芝麻胺将其抑制到正常水平。5)在相同的试验条件下，在缺乏生育酚的饲料中添加绿茶中的儿茶素及其类似物，可以轻微抑制肝脏、肾脏和血浆中的脂质过氧化。