Proliferative effects of humoral factors derive from neuronal cells on cultured astrocytes

从两种类型的培养神经元，未成熟的神经母细胞瘤细胞（NRE细胞）和成熟的原代培养神经元（PCNs）释放的体液因子的生长促进作用，清楚地显示在浓度依赖性的方式对未成熟的胶质细胞，C6细胞。在较高浓度的神经元条件培养液条件下，未观察到生长受限现象。另一方面，在成熟的原代培养的星形胶质细胞（PCA）上，观察到来自神经元条件培养基（NCM）的体液因子以浓度依赖性方式的生长抑制作用。NCM对NRE的抑制作用虽无统计学意义，但有抑制作用的趋势，两种NCM处理均引起C6细胞和PCA的形态学改变。C6和PCA的细胞体均变细，突起变长，两种NCMs处理后C6细胞的胸苷掺入量均显著增加。另一方面，在PCA中，两种类型的NCM处理均抑制胸苷掺入。e.生长促进和抑制作用，对培养的星形胶质细胞。这表明，释放因子的不同作用，增殖或抑制，是依赖于目标星形胶质细胞，不成熟或成熟的条件。

Growth promoting effects of humoral factors released from both types of cultured neurons, immature neuroblastoma cells (NRE cells) and mature primary cultured neurons (PCNs), were clearly shown in concentration dependent manner on immature glial cells, C6 cells. No growth limited phenomenon was observed under the condition of higher concentration of neuron conditioned medium. On mature primary cultured astrocytes (PCAs), on the other hand, growth inhibitory effects of humoral factor (s) from neuron conditioned medium (NCM), was observed in concentration dependent manner. Although statistically significant inhibition was not shown by the treatment of NCM from NRE,tendency of the inhibitory effects were found. Morphological alterations were found by both types of NCM treatments in C6 cells and PCAs. The cell bodies of both C6 and PCAs became slender weth longer processes.Drastic increase in thymidine incorporation was found by the treatment of both types of NCMs in C6 cells. In PCAs, on the other hand, thymidine incorporation were suppressed by the treatment of both types of NCMs.Thus humoral factors from neuronal cells had dual effects, i. e. growth-promoting and -inhibitory actions, on cultured astrocytes. It is suggested that the different effects of the releasing factors, proliferative or inhibitory, are dependent upon the conditions of target astrocytes, immature or mature.