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Dynamics of microglial cells in the brain of Ihara epileptic rats in accordance with seizure progress

Ihara癫痫大鼠脑内小胶质细胞随癫痫发作进展的动态变化

基本信息

批准号：
13670212
负责人：
AMANO Shigeru
金额：
$ 2.11万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

It is supposed that the significance of glial reaction in epileptic brain might be unveiled by clarify the localization and degree of gliosis in epileptic mutant, Ihara epileptic rat. Possible function of microglia cells in the rat experienced epileptic seizures was studied by investigation of localization and degree of microglial reaction in IER brains with immunohitochemical examination using OX-42 antibody. Density of OX-42 positive microglial cells was investigated in the portions of cerebral cortex, endorrhinal cortex, thalamus, amygdale, hippocampus and dentate gyrus in IERs and non-epileptic control rats.In IERs, rats of 2 months old, microglial with OX-42 positive reaction was increased in mild or moderate degree in the portions of the brain described above, particularly in the hippocampus, the thalamus and the amygdala. In rats of 6 months and 10 months old, marked increase in OX-42 positive microglia was found in hippocampus, thalamus and amygdala in addition to the whole brain. Microglia cells with OX-42 positive reaction, which possessed short and thick processes, were thought to be an activated microglial of amoeboid type.It is suggested that microglial activation was induced by epileptic activities in the brain of IER, and then cytokines released by activated glial cells have some influences to the functions of neurons or glial cells. Because increased activated microglia was shown in the portions of hippocampus, thalamus and amygdala from young age, those areas ware thought to have significant role for the epileptogenesis. Furthermore, it is safely assumed that epileptic activities influenced not only in the hippocampus, thalamus and amygdala but also in other portions of the brain from the finding of diffuse increase in OX-42 positive cells in the whole brain of IER.

通过阐明癫痫突变体Ihara癫痫大鼠神经胶质瘤的定位和程度，有望揭示癫痫脑内神经胶质瘤反应的重要意义。应用OX-42抗体免疫组化检查，观察大鼠脑内小胶质细胞的定位和反应程度，探讨小胶质细胞在癫痫发作大鼠脑内的可能功能。研究了ier大鼠和非癫痫对照大鼠大脑皮层、内鼻皮层、丘脑、杏仁核、海马和齿状回部分OX-42阳性小胶质细胞的密度。在2月龄大鼠中，上述脑区，特别是海马、丘脑和杏仁核，OX-42阳性反应的小胶质细胞轻度或中度增加。6月龄和10月龄大鼠除全脑外，海马、丘脑和杏仁核的OX-42阳性小胶质细胞明显增加。OX-42阳性的小胶质细胞具有短而粗的突起，被认为是一种活化的变形虫型小胶质细胞。提示癫痫病患者脑内小胶质细胞激活，激活后胶质细胞释放的细胞因子对神经元或胶质细胞的功能有一定影响。由于海马、丘脑和杏仁核部分被激活的小胶质细胞增加，这些区域被认为在癫痫发生中起重要作用。此外，从发现IER全脑OX-42阳性细胞弥漫性增加来看，可以肯定癫痫活动不仅影响海马、丘脑和杏仁核，还影响大脑的其他部分。