Modes of action and structure of host-selective pathogenicity factors produced by Phytophthora pathogens
Modes of action and structure of host-selective pathogenicity factors produced by Phytophthora pathogens
批准号:
06454061
负责人:
DOKE Noriyuki
金额:
$4.67万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1994
资助国家:
日本
项目状态:
已结题
起止时间:
1994 至 1995
中文摘要
在这个项目中,从致病疫霉(Phytophthora spp.)中分离纯化了一种具有寄主选择性抑制活性的葡聚糖,并对其作用方式进行了分析。结果表明:1)从接种致病疫霉游动孢子的透析管内液和孢子在含Ca ~(2+)蒸馏水中的萌发液中分离到一种磷酸葡聚糖(P-SG); 2)从致病疫霉游动孢子的透析管内液中分离到一种磷酸葡聚糖(P-SG); 3)从致病疫霉游动孢子的透析管内液中分离到一种磷酸葡聚糖(P-SG); 4)从致病疫霉游动孢子的透析管内液中分离到一种磷酸葡聚糖(P-SG); 5)从致病疫霉游动孢子的透析管内液中分离到一种磷酸葡聚糖(P-SG); 6)从致病疫霉游动孢子的透析管内液中分离到一种磷酸葡聚糖(P-SG)。SG的特征在于葡聚糖在18-23聚合葡萄糖,每个分子具有1-4个磷酸盐,以及β-1,3-半乳聚糖酶和磷酸酶敏感的葡聚糖。P-SG在不同pH下通过凝胶过滤柱的高效液相色谱分析得到的P-SG仍由多个葡聚糖分子组成,其结构有待进一步分析; 3)通过检测受试组织的自显影,设计了一种抑制剂活性的检测系统; 4)发现P-SG抑制Ca^<2+> 5)P-SG与寄主质膜表面存在分子相互作用,但这种作用受到质膜悬浮液中葡聚糖酶和磷酸酶的干扰,为进一步研究该抑制剂的作用机制,需要进一步完善体外培养体系。
英文摘要
In this project, isolation and purification of the active host-selective suppressor-glucans from Phytophthora spp.and analyzes of the their mode of action were carried out and the following results were obtained.1) A phospho-glucan (P-SG) was isolated from inner solution of a dialysis tube on which zoospore-suspension of P.infestrans was inoculated as well as spore-germinated fluid in Ca2+ containing destilled water.2) The P-SG was characterized by glucans with polymerization of glucose at 18-23 and with 1-4 phosphate per molecule and beta-1,3-galucanase- and phosphatase sensitive glucans. The P-SG was still consisted of several glucan molecules since it was further separated through HPLC analyzes on gel filtration columns at different pH.The detailed analyzes of their structures were remained to be carried out.3) A assay system for suppressor activity was devised by testing the reduction of elicitorstimulated chemiluminescence on an autophotography of the test tissued.4) P-SG was found to inhibit the Ca^<2+> movement from plasmamembrane which was stimulated by elicitor treatment and resultantly inhibit the activation of the superoxide generating system.5) A molecular interaction between P-SG and surface of host plasmamembrane was tested, but the results was disturbed by the presence of glucanase and phosphatase in the plasmamembrane suspension, The further improvement of the in vitro systemn was requested for analyzes of the molecular mechanisms of the supperessor.
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Yoshioka,H.: "Rapid accumulation of Phenylalanine ammonialyase mRNA and 3-hydroxy-3-methylglutarul CoA reductase mRNA by protein synthesis inhibitors." Plant Cell physiol.35. 1257-1260 (1994)
Yoshioka, H.:“蛋白质合成抑制剂使苯丙氨酸解氨酶 mRNA 和 3-羟基-3-甲基戊二酰辅酶 A 还原酶 mRNA 快速积累。”
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通讯作者:
L.M.Sanchez: "Involvement of suppressor-glucans and plant epidermal cells in host-selective pathogenesis of Phytophthora capsici" J.Phytopathol.140. 153-164 (1994)
L.M.Sanchez:“抑制葡聚糖和植物表皮细胞参与辣椒疫霉宿主选择性发病机制”J.Phytopathol.140。
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Miura.Y.: "An autophotographic determination of the active oxygen generation in potato tuber discs durin hypersensitive responseto fungal infection or elicitor." Plant Sci.105. 45-52 (1995)
Miura.Y.:“对真菌感染或激发子过敏反应期间马铃薯块茎中活性氧产生的自照相测定。”
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道家紀志: "植物の感染防御応答における活性酸素生成と寄生戦略.露無慎二編、植物の感染機構の進化を考える." 日本植物病理学会, 118(86-97) (1995)
Noriyoshi Michie:“植物感染防御反应中的活性氧产生和寄生策略。Shinji Tsuyu,编辑,考虑植物感染机制的演变,118(86-97)(1995)”
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通讯作者:
Yoshioka, H.: "Rapid accumulation of phenylalanine ammonia-lyase mRNA and 3-hydroxy-3-methylglutaryl CoA reductase mRNA by protein synthesis inhibitors." Plant Cell physiol.35. 1257-1260 (1994)
Yoshioka, H.:“蛋白质合成抑制剂导致苯丙氨酸解氨酶 mRNA 和 3-羟基-3-甲基戊二酰辅酶 A 还原酶 mRNA 快速积累。”
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共 26 条
Molecular and physiological studies on oxidative burst and active defense mechanism in plant and its application for disease tolerance
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批准号:14104004
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项目类别:Grant-in-Aid for Scientific Research (S)
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资助金额:$65.06万
-
财政年份:2002
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负责人:DOKE Noriyuki
-
依托单位:
Molecular mechanism or oxidative burst in infected plants and its control mechanism of networks of local nd systemic defense response to infection
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批准号:11306004
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项目类别:Grant-in-Aid for Scientific Research (A)
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资助金额:$27.08万
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财政年份:1999
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负责人:DOKE Noriyuki
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依托单位:
Induction of plant immunization by components of bio-resources and its application for screening of inducers.
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批准号:10556010
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项目类别:Grant-in-Aid for Scientific Research (B).
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资助金额:$8.0万
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财政年份:1998
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负责人:DOKE Noriyuki
-
依托单位:
Molecular and physiological mechanism of oxidative burst in relation to signal transduction for defense response of plant to infection
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批准号:09460027
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.96万
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财政年份:1997
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负责人:DOKE Noriyuki
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依托单位:
Intercellular recognition and signal transduction in plants
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批准号:06304023
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项目类别:Grant-in-Aid for Co-operative Research (A)
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资助金额:$10.88万
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财政年份:1994
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负责人:DOKE Noriyuki
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依托单位:
ENHANCEMENTS OF DISEASE RESISTANCE AND PRODUCTIVITY IN POTATO PLANTS BY INDUCTION TREATMENT OF PLANT IMMUNIZATION
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批准号:03556007
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$9.22万
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财政年份:1991
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负责人:DOKE Noriyuki
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依托单位:
Signal transduction in induction of bio-defense and pathogen-reception in plants infected with fangi
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批准号:02454051
-
项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.78万
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财政年份:1990
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负责人:DOKE Noriyuki
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依托单位: