Molecular and physiological studies on oxidative burst and active defense mechanism in plant and its application for disease tolerance

植物氧化爆发和主动防御机制的分子生理学研究及其抗病应用

• 批准号: 14104004
• 负责人: DOKE Noriyuki
• 金额: $ 65.06万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14104004/
• 关键词: oxidative burst; networks of defense response; O_2^- generating NADPH oxidase; MAP kinase cascade; generation of disease tolerant potato; PVS3 promoter; NO generating nitrate reductase; systemic signal transduction; rboh; CDPK; PVS3; ZAT12; 耐病

This project made it clear the followings on molecular mechanisms of oxidative burst (OXB) and active defense-network in Phytophtora infestans-infected potato plants. 1)In the course of the defense response, immediately-responsive OXB and induced OXB, which were dependent on superoxide-generating NADPH oxidase, RbohA and RbohB, were induced and then played a key signal role in the induction of HR-type defense response. 2)RbohA was activated through Ca^<2+> influx and a Ca^<2+>-dependent protein kinase, whereas RbohB was induced under control of MAPK cascade, and then activated by CDPK in relation to the 1st OXB. 3)A transient expression of MEK1^<DD> of MAPKK, that was modified by amino acid exchange to be constitutively active, caused activation of SIPK and WIPK of MAPK, induction of RbohB, HMGR,PVS etc. and then induction of HR-type defense response. 4)Activated MPK1 phosphorylated multiple host proteins (PPS1-8),PPS3 of which regulated expressions of RbohB, HMGR, and PVS. 5)Compatible races also induced activation of MAPK cascade and induction of non-active RbohB,HMGR and PVS, and stimulate infection-specific GUS expression in transgenic potato with PVS3 promoter-linked GUS gene. 6)NO generation was found to be accompanied by the 1st OXB, playing an important role in induction of HR-type response. 7)The NO generation was dependent on inducible-nitrate reductase (NR) and other unknown enzyme. 8)Local OXB stimulated a sequential Ca^<2+> influx among inner tissue cells, and this signal came to stimulate systemic OXB in the surface tissue, following expression of RbohB and PAL for induction of systemic acquired resistance (SAR). 9)Transgenic potato plants with PVS3 promoter-linked MEK^<DD> demonstrated tolerance to virulent P.infestans and Alternaria solani with HR-type defense response.

本课题对致病疫霉侵染马铃薯植株氧化猝发(OXB)和主动防御网络的分子机制进行了研究。1)在防御反应过程中，诱导了依赖于超氧化物产生的NADPH氧化酶、RbohA和RbohB的即刻反应型OXB和诱导型OXB，并在HR型防御反应的诱导中发挥了关键的信号作用。2)RbohA是通过钙内流和依赖于钙的蛋白激酶激活的，而RbohB是在MAPK级联调控下被激活的，然后被CDPK激活，涉及第一个OXB。3)MAPKK的瞬时表达，经氨基酸交换修饰为结构性活性后，可激活MAPK的SIPK和WIPK，诱导RbohB、HMGR、PVS等，进而诱导HR型防御反应。4)激活的MPK1磷酸化多宿主蛋白(PPS1-8)，其中PPS3调节RbohB、HMGR和PVS的表达。5)亲和小种还诱导了MAPK级联激活和非活性RbohB、HMGR和PVS的诱导，并刺激了PVS3启动子连锁的GUS基因转基因马铃薯的侵染特异性GUS表达。6)没有发现伴随第一个OXB的生成，这在诱导HR型反应中起着重要作用。7)NO的生成依赖于诱导型硝酸还原酶(NR)和其他未知的酶。8)局部OXB刺激组织内细胞内钙离子顺序性内流，该信号继RbohB和PAL表达后刺激表层组织中的系统OXB，从而诱导系统获得性抗性(SAR)。9)转PVS3启动子的马铃薯植株表现出对致病疫霉和茄病链格孢菌的耐性，表现出HR类型的防御反应。

项目成果

A strategy to enhance disease resistance of potato using the mechanism of the hypersensitive reaction against potato late blight.

利用马铃薯晚疫病过敏反应机制增强马铃薯抗病性的策略。

• 发表时间: 2005
• 期刊: J. Gen. Plant Pathol. 71
• 作者: Doke;N.
• 通讯作者: N.

宿主植物の抵抗性制御と糸状菌病に対する耐病性強化の展望.

控制寄主植物抗性和增强真菌病害抗性的前景。

• 发表时间: 2003
• 期刊: 植物感染生理談話会論文集 : 作物の耐病性強化戦略と植物-病原体相互作用の分子機構 39
• 作者: Mabuchi;H;吉岡博文
• 通讯作者: 吉岡博文

植物における活性酸素の役割とその誘導

活性氧在植物中的作用及其诱导

• 发表时间: 2006
• 期刊: 蛋白質 核酸 酵素 50
• 作者: T.Ujihara;K.Obara;N.Usami;K.Fujiwara;G.Sazaki et al.;吉岡博文
• 通讯作者: 吉岡博文

Elicitation of primary and secondary metabolism during defense in the potato.

• DOI: 10.1007/s10327-003-0075-6
• 发表时间: 2003-12-01
• 期刊: Journal of General Plant Pathology
• 影响因子: 1.2
• 作者: Nakane, Eiichi;Kawakita, Kazuhito;Yoshioka, Hirofumi
• 通讯作者: Yoshioka, Hirofumi

植物免疫としてのオキシダティブバーストを探る-MAPKカスケードが活性酸素生成と細胞死を制御する.

探索氧化爆发作为植物免疫 - MAPK 级联控制活性氧的产生和细胞死亡。

• 发表时间: 2003
• 期刊: 化学と生物 41(10)
• 作者: K.Nakajima;N.Usami;K.Fujiwara;Y.Murakami;T.Ujihara;G.Sazaki;T.Shishido;A.Hashidzume;Chutiporn Anutariya;吉岡博文
• 通讯作者: 吉岡博文