Intercellular recognition and signal transduction in plants

植物细胞间识别和信号转导

• 批准号: 06304023
• 负责人: DOKE Noriyuki
• 金额: $ 10.88万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06304023/
• 关键词: Recognition of elicitor; Oxidative burst; Ca^<2+> dependent protein kinase; Active electronic potential; Cytoskeleton; Systemic acquired resistance; phospholipase; Sub-systemic oxidative burst; タンパク質リン酸化酵素; ホスホリパーゼA_2; 全身的獲得抵抗性; 電気的シグナル; システミッ

In this research project, recognition and signal transduction in a cell as well as tissue-cells were investigated using various systems of plant-microbe, biotic elicitor or physical stimuli, and the following major results were obtained.1) In relation to Ca ^<2+>signalling, effect of Mg ^<2+>on the Ca ^<2+>/H+ antiporter or Ca^<2+>, purification and characterization of Ca^<2+>dependent protein kinase and involvement of Ca ^<2+>dependent protein kinase in inositol phospholipid turnover and PAL induction in elicitor-treated suspension cultured cells was demonstrated. 2) N-acetylchitoorigosaccharide elicited an oxidative burst generating OH radical and phosphorylation of the various proteins in rice suspension cultured cells. 3) Active electric potential was generated and transduced in characean cells by a physical stimulus and resulted in cause a cessation of protoplasmic streaming by Ca^<2+> dependent phosphorylation of myosin. 4) Fungal elicitors stimulated the oxidative burst, followed by lipid peroxidation with an activation of phospholipase A^2. This enzyme activity in potato tissues was dependent on CaM and protein kinase and appeared to be patatin. 5) Cytoskeleton was involved as some signal transduction in rejection-response of plant cells against invading pathogens. 6) In induction of systemic acquired resistance in barley seedlings to pathogens, infection or wounding signals was found to cause first a electric signal and then production of some molecules for secondary signal. 7) The oxidative burst in potato tissues treated with fungal elicitor was found to be dependent on Ca^<2+> influx, CaM and protein kinase and to cause a subsystemic oxidative burst by generated H_2O_2. The subsystemic oxidative burst was also due to Ca^<2+> signalling.

本研究利用植物-微生物、生物激发子或物理刺激等多种系统，对细胞和组织细胞的识别和信号转导进行了研究，得到了以下主要结果：1)与钙信号转导有关，镁离子对钙离子逆向转运体或钙离子逆向转运蛋白的影响，钙离子依赖性蛋白激酶的纯化和性质，以及钙离子的参与。在激发子处理的悬浮培养细胞中，肌醇磷脂转化和PAL诱导中的依赖蛋白激酶被证明。2)N-乙酰基壳聚糖在水稻悬浮培养细胞中引起氧化猝发，产生羟基自由基和各种蛋白质的磷酸化。3)在物理刺激的作用下，细胞内产生并传递激活电位，并通过钙依赖的肌球蛋白磷酸化导致原生质流的停止。4)真菌激发子刺激氧化爆发，随后发生脂质过氧化，激活磷脂酶A^2。马铃薯组织中磷脂酶A^2的活性依赖于CaM和蛋白激酶，表现为Patatin。5)细胞骨架参与了植物细胞对入侵病原菌的排斥反应。6)在诱导大麦幼苗对病原菌的系统获得性抗性中，侵染或伤害信号首先引起电信号，然后产生一些次生信号分子。7)经真菌激发子处理的马铃薯组织中的氧化猝发依赖于Ca~(2+)内流、CaM和蛋白激酶，并通过产生H_2O_2而引起亚系统氧化猝发。亚系统氧化猝发还与Ca~(2+)&gt；

项目成果

T. Yuasa: "Purification and characterization of a Ca^<2+>-dependentprotein kinase from the halotolerant green alga Dunaliella tertiolecta." Plant Cell Physiol.34. 699-708 (1995)

T. Yuasa：“来自耐盐绿藻 Dunaliella tertiolecta 的 Ca^2 依赖性蛋白激酶的纯化和表征。”

Y. Kamada: "Protein kinase inhibitors inhibit stimulation of inositol phospholipid turnover and induction of phenylalanine ammonia-lyase in fungal elicitor-treated tobacco suspensioncultured cells." Plant Cell Physiol.35. 405-409 (1994)

Y. Kamada：“在经真菌激发子处理的烟草悬浮培养细胞中，蛋白激酶抑制剂可抑制肌醇磷脂周转的刺激和苯丙氨酸解氨酶的诱导。”

T. Shimmen: "Unique after-hyperpolarization accompanying action potential in Chara globularis." J. Plant Res.107. 371-375 (1994)

T. Shimmen：“球轮轮中独特的后超极化伴随动作电位。”

T.Wayne: "The relation between carbon and water transport in single cells of Chara corallina" Protoplasma. 180. 118-135 (1994)

T.Wayne：“珊瑚轮藻单细胞中碳和水运输的关系”原生质体。

T.Simmen: "Studies on mechanoperception in characean cells : development of apparatus." J.Plant Res.(in press). (1995)

T.Simmen：“对轮虫细胞机械知觉的研究：装置的发展。”