CELL-PHYSIOLOGICAL STUDY ON THE INHIBITORY ACTIONS OF VOLATILE FATTY ACIDS ON THE GH RELEASE FORM RUMINANT SOMATOTROPES

挥发性脂肪酸对反刍动物生长激素释放形式抑制作用的细胞生理学研究

• 批准号: 06454111
• 负责人: KATOH Kazuo
• 金额: $ 4.16万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454111/
• 关键词: GH Release; Somatotropes; Ruminant; Calcium Channel; Calcium Ion; Calcium Current; カルシウムチャネル; VFA; GH; ソマトトロフ; カルシウム電流; ヤギ; GRF; GHRP

We obtained the following results with caprine and ovine anterior pituitary cells :1)A rise in GH release from ovine and caprine anterior pituitary cells in response to GRF or GHRP stimulation was significantly inhibited by an addition of VFA in the medium (acetate, propionate and butyrate : 10 mM), where the cells were perifused immediately after cell dispersion or were cultured for 3 days and incubated in the culture dishes.2)The antagonist for L-type calcium channels (nifedipine) completely inhibited, while the antagonist for the non-specific cation channels (tetramethrine) never affected, the GH release induced by GRF stimulation.3)We got the following results using the whole-cell patch clamp method : A.Application of depolarization pulse to the cultured cells caused an increase in inward current at the holding potential was set at - 20 mV.B.The inward current was completely abolished by an addition of nifedipine or fatty acids in the bath medium. Form these results we conclude that volatile fatty acids inhibit the GH release induced by GRF or GHRP stimulation, which is probably due to the inhibitory effects of volatile fatty acids on calcium channels on ruminant somatotropes.

在绵羊和绵羊脑垂体细胞上，我们得到以下结果：1)在细胞分散后立即灌流或培养3天后加入VFA，可显著抑制绵羊和绵羊脑垂体细胞对GRF或GHRP刺激的GH释放增加；2)L型钙通道拮抗剂(硝苯地平)完全抑制GH的释放，而非特异性阳离子通道拮抗剂(四甲基平)则不受影响。3)应用全细胞膜片钳方法，我们得到了以下结果：A.施加去极化脉冲，使保持电位处的内向电流增加至-20 mV.B.，加入硝苯地平或脂肪酸可完全消除内向电流。这些结果表明，挥发性脂肪酸抑制了GRF或GHRP刺激引起的生长激素释放，这可能是由于挥发性脂肪酸对反刍动物生长激素上钙通道的抑制作用。

项目成果

Katoh, K.: "Effects of GHRP on GH release and intracellulay calcium concentration in primary cultured ovine anterior pituitary cells" Journal of Reproduction and Development. (1996)

Katoh, K.：“GHRP 对原代培养的绵羊垂体前叶细胞中 GH 释放和细胞内钙浓度的影响”《生殖与发育杂志》。

Wu, D.: "The effects of GH-releasing peptide-2 (GHRP-2 or KP102) on GH secretion from primary cultured ovine pituitary cells can be abolished by a specific GRF receptor antagonist" Journal of Endocrinology. 140. R9-R13 (1994)

Wu, D.：“GH 释放肽 2（GHRP-2 或 KP102）对原代培养的绵羊垂体细胞 GH 分泌的影响可以通过特定的 GRF 受体拮抗剂消除”《内分泌学杂志》。

Wu, D: "The effects of GH-releasing peptide-2 (GHRP or KP102) on GH secretion from primary cultured pituitary cells can be abolished by a specific GRF receptor antagonist" Journal of Endocrinology. 140. R9-R13 (1994)

Wu, D：“GH 释放肽 2（GHRP 或 KP102）对原代培养的垂体细胞 GH 分泌的影响可以通过特定的 GRF 受体拮抗剂消除”《内分泌学杂志》。

Katoh, K.: "Effects of GHRP on GH release and intracellular calcium concentration in primary cultured ovine anterior pituitary cells" Journal of Reproduction and Development. (1996)

Katoh, K.：“GHRP 对原代培养的绵羊垂体前叶细胞中 GH 释放和细胞内钙浓度的影响”《生殖与发育杂志》。

Wu, D.: "Effects in vitro of new growth hormone relessing peptide (GHRP-1) on growth hormone secretion from ovine pituitary cells in primary culture" Journal of Seuroendocrinology. 6. 185-190 (1994)

Wu, D.：“新型生长激素释放肽（GHRP-1）对原代培养中绵羊垂体细胞生长激素分泌的体外影响”《神经内分泌学杂志》。