Physiological and molecular biological study on the mechanisms for VFA-induced inhibition of GH release from somatotrophs

VFA抑制生长激素释放GH机制的生理和分子生物学研究

• 批准号: 09460122
• 负责人: KATOH Kazuo
• 金额: $ 3.39万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460122/
• 关键词: VFA; GH (GROWTH HORMONE); GH SECRETING CELLS; SOMATOTROPHS; 分泌

(1) Effects of VFA on the basal and GHRH-induced GH releaseWhen the cells incubated with butyrate or propionate for 72 hrs were stimulated with GHRH, GH release was significantly suppressed compared with the cells cultured in a medium without a fatty acid.(2) Effects of VFA on membrane Ca currentWhen membrane potential was depolarized in a step manner, inward current was increased via the cell membrane. This current, which was completely abolished by nifedipine, was suppressed in a medium containing butyrate.(3) Effects of VFA on cellular Ca ion concentrationsButyrate itself slightly increased, but it rater suppressed GHRH-induced increase in cellular Ca concentration.(4) Effects of VFA on GHmRNA contentButyrate suppressed GHmRNA content.

(1)VFA对基础生长激素释放和GHRH诱导生长激素释放的影响与不含脂肪酸的培养液相比，GHRH刺激丁酸盐或丙酸盐孵育72小时的细胞时，生长激素释放明显受到抑制。(2)VFA对细胞膜Ca电流的影响当膜电位阶跃去极化时，通过细胞膜的内向电流增加。这种电流，这是完全废除硝苯地平，抑制在培养基中含有丁酸。(3)VFA对细胞内Ca ~（2+）浓度的影响丁酸本身对细胞内Ca ~（2+）浓度有轻微的升高作用，但对GHRH引起的细胞内Ca ~（2+）浓度升高有一定的抑制作用。(4)VFA对GHmRNA含量的影响丁酸抑制GHmRNA的含量。

项目成果

Matsunaga, N., Arakawa, N.T., Goka, T., Nam, K.-T., Ohneda, A., Sasaki, Y.and Katoh, K.: "Effects of ruminal infusion of volatile fatty acids on plasma concentration of growth hormone and insulin in sheep" Domestic Animal Endocrinology. (in press).

Matsunaga, N.、Arakawa, N.T.、Goka, T.、Nam, K.-T.、Ohneda, A.、Sasaki, Y. 和 Katoh, K.：“瘤胃输注挥发性脂肪酸对血浆浓度的影响

Ohataら: "Growth hormone release Induced by an amino acid mixture from primary cultured anterior pitultary cells of goats" Domestic Animal Endocrinology. 14. 99-107 (1997)

Ohata 等人：“由原代培养的山羊垂体前细胞的氨基酸混合物诱导的生长激素释放”家畜内分泌学 14. 99-107 (1997)。

Ohata, Y., Maruyama, Y., Katoh, K.and Sasaki, Y.: "Growth hormone release induced by an amino acid mixture from primary cultured anterior pituitary cells of goats." Domestic Animal Endocrinology. 14 (2). 99-107 (1997)

Ohata, Y.、Maruyama, Y.、Katoh, K. 和 Sasaki, Y.：“原代培养的山羊垂体前叶细胞的氨基酸混合物诱导生长激素释放。”

Katoh, K., Ohata, Y.and Ishiwata, H.: "Suppressing effects of short-chain fatty acids on GHRH-induced GH release in isolated anterior pituitary cells of goats." Domestic Animal Endocrinology. (in press).

Katoh, K.、Ohata, Y. 和 Ishiwata, H.：“短链脂肪酸对山羊离体垂体前叶细胞中 GHRH 诱导的 GH 释放的抑制作用。”

Ohata et al.: "Growth hormone release induced by an amino acid mixture from primary cultured anterior pituitary cells of goats." Domestic Animal Endocrinology. 14(2). 99-107 (1997)

Ohata 等人：“原代培养的山羊垂体前叶细胞的氨基酸混合物诱导生长激素释放。”