Visualization of expression of gene increased the unsaturated fatty acids in meat

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This study was to 1) investigate the gene expression of SCD in bovine adipocytes, and 2) generate delta-9-desaturease-GFP- knock in mice to visualize the activation of an enzyme generating a flavor and taste of the intramuscular fat.1) Bovine GH (100 and 1000 ng/ml) decreased SCD mRNA expression in bovine differentiated adipocytes. 2) Using the homologous recombination method, embryo stem cell derived from the mouse embryo with GFP protein in an Exon1 of the internal delta-9 desaturase was established. The chimera mouse with delta-9-desaturease-GFP was identified and hetero delta-9-desaturease-GFP mouse generated.