Construction of High-Rate Anaerobic Process in Treating Aromatic Compounds-Containing Wastewater

采用间歇厌氧毒性试验，研究了苯甲酸、苯酚、邻苯二酚、间苯二酚、对苯二酚、间苯三酚等6种芳香族化合物对产乙酸甲烷菌的抑制作用。同时，利用驯化苯酚的厌氧污泥进行间歇厌氧毒性试验，研究了苯酚的降解特性和代谢途径。根据结果，可以得出以下结论：1）添加芳香族化合物后剩余的相对产甲烷活性随着测试的所有芳香族化合物浓度的增加而降低。苯<50>甲酸、苯酚、邻苯二酚、间苯二酚、对苯二酚和间苯三酚对产甲烷活性的50%抑制浓度（IC）分别为49.2，18.6，22.7，27.7，40.0和51.6 mmol/L。辛醇/水分配系数（logP）的对数与产甲烷活性的抑制率呈线性关系，其方程为：logIC_<50>=-0.283logP +1.68（n=5，r^2=0.953）3）苯酚对苯酚厌氧降解速率的影响可用Haldan方程表示。动力学参数k、K_s和K_i值分别为0.096hr ~<-1>（-1）、1128 mg/L和188 mg/L。苯酚主要代谢为苯甲酸盐。苯甲酸盐进一步转化为脂肪酸，其随后矿化为乙酸盐和氢。苯甲酸盐和乙酸盐是苯酚厌氧降解的主要代谢产物。

The inhibitory effects of six kinds of aromatic compounds (benzoate, phenol, catechol, resorcinol, hydroquinone, phloroglucinol) on the acetotrophic methanogenesis were investigated by batch anaerobic toxicity assays. Concomitantly, the degradation characteristics and metabolic pathway were clarified by batch anaerobic toxicity assays using anaerobic sludge acclimatized to phenol. Based on the results, the following conclusions can be made :1)The relative methanogenic activity remaining after addition of aromatic compounds decreased with an increase in the concentration of all aromatic compounds tested. The concentrations (IC_<50>) of benzoic acid, phenol, catechol, resorcinol, hydroquinone and phloroglucinol resulting in 50% inhibition of methanogenic activity were 49.2,18.6,22.7,27.7,40.0, and 51.6 mmol/L,respectively.2)For all the phenolic compounds tested, a good correlationship between their inhibition effect and hydrophobicity was observed, and the logarithm of the octanol/water partition coefficient (logP), an indicator of hydrophobicity, was found to be correlated with the inhibition of methanogenic activity, according to the following equation : logIC_<50>=-0.283 logP+1.68 (n=5, r^2=0.953)3)The effect of phenol on the anaerobic degradation rate of phenol was represented by Haldan equation. The values of k, K_s and K_i as kinetic parameter were 0.096hr^<-1>,1128mg/L and 188mg/L,respectively. Phenol was metabolized primarily into transformed into benzoate. Benzoate was further converted to fatty acids which are subsequently mineralized to acetate and hydrogen. Benzoate and acetate were major metabolites of anaerobic degradation of phenol.