Differentiation of a human megaryoblastic cell line (MEG-01) and its production of platelets and platelet-derived factors

我们已经建立了一个新的人巨核细胞系（MEG-01）从患者的急变的慢性粒细胞白血病。利用该细胞系，我们获得了以下结果。(1)MEG-01细胞在形态学和表型上通过佛波酯二酯TPA分化为更成熟的巨核细胞。(2)MEG-01细胞在其胞浆中具有凝血因子<VIII>、<V><XII>和<XIII>以及蛋白S。蛋白S是维生素K依赖性凝血因子之一，由MEG-01产生。其功能和分子量（84，000）与正常人血浆中的相同。它的产生被维生素K增强，被华法林抑制。(3)血小板特异性蛋白-<beta>-血小板球蛋白存在于无血清培养基中MEG-01细胞的培养上清液中。(4)使用MEG-01作为免疫原，通过杂交瘤技术产生小鼠单克隆抗体（MG-2）。MG-2（IgM）与MEG-01细胞和人正常巨核细胞特异性反应。

We have established a novel human megakaryoblastic cell line (MEG-01) from a patient with blast crisis of chronic myelogenous leukemia. Using this cell line, we fhave obtained following results. (1) MEG-01 cells are differenciated morphologically and phenotypically to more mature megakaryocytes by a phorbor diester, TPA. (2) MEG-01 cells possess coagulation factors <VIII> , <V> , <XII> and <XIII> as well as protein S in their cytoplasma. Protein S, one of vitamin-K-dependent coagulation factors, is produced by MEG-01. Its function and molecular weight (84,000) is the same as one in normal human plasma. Its production was enhanced by vitamin-K and suppressed by warfarin. (3) A platelet specific protein, <beta> -thromboglobulin, is present in the culture supernatant of MEG-01 cells in serum-free medium. (4) A mouse monoclonal antibody (MG-2) was produced by the hybridoma technique using MEG-01 as an immunogen. MG-2 (IgM) reacts specifically with MEG-01 cells and human normal megakaryocytes.