The study for new therapy of endotoxin shock

我们能够通过缩合或诱导其与卤素反应来固定多粘菌素B。在缩合反应后，一克聚苯乙烯纤维上附着的多粘菌素B的剂量比卤素反应后要大得多。发现固定多粘菌素B牢固地附着在纤维上。注射含有内毒素的PMX-F处理液后小鼠的死亡率表明，处理组有24%（21只中的5只）存活。而未经治疗的21人中有21人100%死亡。在离体实验中，对内毒素注射犬进行了PMX-F直接血液灌流（DHP）。对照组只有12.5%（1 / 8）存活，而接受DHP + PMX-F治疗组有83%（10 / 12）存活。治疗组死亡率明显降低。结果表明，PMX-F具有中和内毒素的作用。对于临床应用，PMX-F正在评估血液相容性与大柱人。使用PMX-F检测DHP时，肝素用量比早期检测时少。该剂量的肝素在动物体内可安全驱动DHP。

We were able to immobilize Polymyxin B through condensation or by inducing in to react with halogen. A much larger dose of Polymyxin B adhered to one gr of polystyrene fiber after the condensation reaction than it did following the halogen reaction. Fixed polymyxin B was found to be firmly attached to the fiber. The mortality of mice after injection of PMX-F treated fluid contained endotoxin, indicated that 24 % (5 of 21) of the treated group survived. Whereas 100 % (21 of 21) of the untreated group died. In ex vivo experiments, direct hemoperfusion (DHP) by PMX-F was performed for endotoxin-injected canine. Only 12.5 % (1 of 8) survived in the control group, but 83 % (10 of 12) survived in the treated group receiving DHP with PMX-F. Mortality in the treated group decreased remarkably. The results thus indicated the efficacy of PMX-F in neutralizing endotoxin.For the clinical application, PMX-F is being estimated blood compatibility with larger column for human. And DHP using PMX-F is tried by smaller amount of heparin than that of earlier examination. DHP can be driven safely with this quantity of heparin in animal.