The study for new therapy of endotoxin shock

内毒素休克新疗法的研究

• 批准号: 60480291
• 负责人: KODAMA Masashi
• 金额: $ 3.52万
• 依托单位: Shiga university of medical science school of medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480291/
• 关键词: endotoxin; endotoxin shock; polymyxin B; lipopolysacharide; sepsis; direct hemoperfusion; septic shock; ポリミキシンB

We were able to immobilize Polymyxin B through condensation or by inducing in to react with halogen. A much larger dose of Polymyxin B adhered to one gr of polystyrene fiber after the condensation reaction than it did following the halogen reaction. Fixed polymyxin B was found to be firmly attached to the fiber. The mortality of mice after injection of PMX-F treated fluid contained endotoxin, indicated that 24 % (5 of 21) of the treated group survived. Whereas 100 % (21 of 21) of the untreated group died. In ex vivo experiments, direct hemoperfusion (DHP) by PMX-F was performed for endotoxin-injected canine. Only 12.5 % (1 of 8) survived in the control group, but 83 % (10 of 12) survived in the treated group receiving DHP with PMX-F. Mortality in the treated group decreased remarkably. The results thus indicated the efficacy of PMX-F in neutralizing endotoxin.For the clinical application, PMX-F is being estimated blood compatibility with larger column for human. And DHP using PMX-F is tried by smaller amount of heparin than that of earlier examination. DHP can be driven safely with this quantity of heparin in animal.

我们能够通过缩合或通过引入与卤素反应来制备多粘菌素B。在缩合反应后，粘附到1克聚苯乙烯纤维上的多粘菌素B的剂量比在卤素反应后大得多。发现固定的多粘菌素B牢固地附着在纤维上。注射含有内毒素的PMX-F处理的液体后小鼠的死亡率表明，处理组中有24%（21只中的5只）存活。而未治疗组100%（21/21）死亡。在离体实验中，通过PMX-F对注射内毒素的犬进行直接血液灌流（DHP）。对照组中仅12.5%（1/8）存活，但接受DHP和PMX-F的治疗组中83%（10/12）存活。治疗组病死率明显降低。因此，PMX-F具有中和内毒素的作用，为临床应用，PMX-F正在进行人体血液相容性评价。使用PMX-F的DHP比早期检查的肝素量更少。在动物体内使用此量的肝素可以安全地驱动DHP。

项目成果

小玉正智 他: 人工臓器.

Masatomo Kodama 等人：人造器官。

小玉正智 他: 人工臓器. 14. 590-594 (1985)

Masatomo Kodama 等：人工器官。14. 590-594 (1985)

小玉正智 他: 免疫と血液. 7. 427-432 (1985)

Masatomo Kodama 等人：免疫与血液。7. 427-432 (1985)

M.Kodama;T.Yoshioka;Y.Endo;T.Tani;K.Hanasawa;T.Oka;K.Matsuda: Journal of Immunology and Immunopharmacology. 6. 224 (1986)

M.Kodama；T.Yoshioka；Y.Endo；T.Tani；K.Hanasawa；T.Oka；K.Matsuda：免疫学和免疫药理学杂志。

M. Kodama et al.: "Studies on Prevention to MOF -Removal of Endotoxin in Serum on Hepatic Disorder" Jpn. J. Artif Organs. 14. 590-594 (1985)

M. Kodama等：“预防MOF的研究-肝脏疾病血清中内毒素的去除”Jpn。