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Studies on the functions of the actirated lymphocytes after partial hepatectomy

肝部分切除术后活化淋巴细胞的功能研究

基本信息

批准号：
60480293
负责人：
TANAKA Noriaki
金额：
$ 2.24万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

We observed that activity of natural killer (NK) cells and in vitro inducibility of lymphokine activated killer (LAK) cells were augmented by partial hepatectomy (HEP) in the spleen cells of C3H mice. LAK cells induced from spleen cells of hepatectomized mice (HEP-LAK) were cytotoxic not only against tumor cell lines but also against parenchymal cells of regenerating liver (hepatoblasts) and lectin induced lymphoblasts. Further more, it was found that IL-2 production of spleen cells was also augmented by HEP. Therefore, we examined the possibility that LAK cells cytotoxic against hepatoblasts are induced in vivo in the regenerating liver. Non-parenchymal cells of liver and spleen cells obtained from normal mice exhibited little cytotoxicity against hepatoblasts. The activities were remarkably augmented by daily administration of IL-2 after HEP. Non-parenchymal liver cells of rat in which parenchymal cells are completely eliminated by density gradient centrifugation exhibited high cytotoxic activity against hepatoblasts. When rats were previously subjected to splenectomy, the activity of non-parenchymal cells after HEP were weakened. Cytostatic activity of HEP-LAK cells was examined in vivo experimental systems. HEP-LAK cells injected intradermaly suppressed epidermal proliferation stimulated by cholera toxin. HEP-LAK cells injected intravenously suppressed Tritium thymidine uptake of the regenerating liver. From above results, it was suggested that lymphocytes are able to regulate proliferation of normal epithelial cells including epidermal cells and liver cells, and play a role of auto-organization.

本文观察到肝部分切除（HEP）可增强C_3H小鼠脾细胞的自然杀伤（NK）细胞活性和淋巴因子激活的杀伤（LAK）细胞的体外诱导。从肝切除小鼠脾细胞诱导的LAK细胞（HEP-LAK）不仅对肿瘤细胞系有细胞毒作用，而且对再生肝实质细胞（肝母细胞）和凝集素诱导的淋巴母细胞也有细胞毒作用。此外，还发现HEP还能促进脾细胞产生IL-2。因此，我们研究的可能性，LAK细胞对肝细胞的细胞毒性诱导在体内再生肝脏。从正常小鼠获得的肝和脾细胞的非实质细胞对成肝细胞几乎没有细胞毒性。HEP后每天给予IL-2可显著增强其活性。用密度梯度离心法去除肝实质细胞的非肝实质细胞对成肝细胞具有较高的细胞毒活性。当大鼠预先进行脾切除，非实质细胞的活性减弱后，HEP。在体内实验系统中检测HEP-LAK细胞的细胞抑制活性。皮内注射HEP-LAK细胞抑制霍乱毒素刺激的表皮增殖。静脉注射HEP-LAK细胞抑制再生肝的氚胸苷摄取。提示淋巴细胞对正常上皮细胞包括表皮细胞和肝细胞的增殖具有调节作用，并具有自组织作用。