Development of Regenerative Medicine Using Reversibly Proliferating Human Bone Marrow Mesenchymal Stem Cells

利用可逆增殖的人骨髓间充质干细胞开发再生医学

• 批准号: 15390378
• 负责人: TANAKA Noriaki
• 金额: $ 9.41万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390378/
• 关键词: human bone marrow mesenchymal stem cells; osteocyte; adipocyte; chondrocyte; lentiviral vector; mouse embryonic stem cells; 骨髄間葉系幹細胞; HGF

Researchers have paid close attention to human bone marrow mesenchymal stem cells(HBMSCs), because HBMSCs have potentials to differentiate to various types of tissue functional cells. Using a retroviral vector SSR#197, encoding LoxP-flanked human telomerase reverse transcriptase and green fluorescence protein cDNAs, we have successfully established an immortalized HMBMSC line YKNT-12. YKNT-12 cells were able to proliferate unlimitedly, on the other hand, normal HBMSCs stopped their proliferation at population doubling 30, indicating a limited life. YKNT-12 cells efficiently differentiated to osteocytes, adipocytes, and chondrocytes by adding specific growth factors in the culture medium, respectively. To differentiate YKNK-12 cells to other-typed cells, genetic manipulation is an important. Thus, we have investigated the usefulness of a lentiviral vector for such gene manipulation in mouse embryonic stem cells(ECs). After lentiviral transduction, mouse ECs still obtained multi-differentiation potentials. Therefore, a lentiviral vector will be one of the useful tools in gene manipulation in YKNK-12 cells and human ECs.

人骨髓间充质干细胞（human bone marrow mesenchymal stem cells, HBMSCs）具有向各种类型的组织功能细胞分化的潜力，因此受到了研究人员的密切关注。利用逆转录病毒载体SSR#197，我们成功地建立了永生化的HMBMSC系YKNT-12，该载体编码loxp -侧翼的人类端粒酶逆转录酶和绿色荧光蛋白cdna。YKNT-12细胞能够无限增殖，另一方面，正常HBMSCs在群体翻倍30时停止增殖，表明寿命有限。通过在培养基中添加特定生长因子，YKNT-12细胞分别高效分化为骨细胞、脂肪细胞和软骨细胞。为了将YKNK-12细胞分化为其他类型的细胞，基因操作是一个重要的方法。因此，我们研究了慢病毒载体在小鼠胚胎干细胞（ECs）中进行这种基因操作的有效性。小鼠内皮细胞经慢病毒转导后仍具有多分化潜能。因此，慢病毒载体将成为对YKNK-12细胞和人内皮细胞进行基因操作的有效工具之一。

项目成果

間葉系幹細胞による骨や脂肪に再生

间充质干细胞再生为骨骼和脂肪

• 发表时间: 2004
• 期刊: 化学工業 55(7)
• 作者: Takada;M;Tada M;Tamoto E;Kawakami A;Murakawa K;Shindoh G;Teramoto K-I;Matsunaga A;Komuro K;Kanai M;Fujiwara Y;Shirata K;Nishimura N;Miyamoto M;Okushiba S-I;Kondo S;Hamada J-I;Katoh H;Yoshiki T;Moriuchi T;小林直哉
• 通讯作者: 小林直哉

Regeneration of bone and fat using mesenchymal stem cells.

使用间充质干细胞再生骨骼和脂肪。

• 发表时间: 2004
• 期刊: Chemical Industry 55(7)
• 作者: Naoya Kobayashi

小林直哉 他: "Cre/loxPシステム"Surgery Frontier. 10(3). 62-65 (2003)

Naoya Kobayashi 等人：“Cre/loxP 系统”Surgery Frontier 10(3) (2003)。