THE IMMUNOLOGICAL HYPORESPONSIVENESS AND DONOR CELL PERSISTENCE IN HOST LIVER AFER PORTAL VENOUS INNOCULATION

免疫抑制剂现在在移植中使用的日子并不只是不完全地抑制了拒绝，但也有一些复杂性，如对抗性感染、恶性肿瘤等。催眠或没有对抗原被称为免疫耐受性的反应，这表明免疫抑制剂的减少受到的拒绝和结果的影响较低或没有。另一方面，捐赠者抗原的门户本质干扰被报道为受捐赠者--动物模型中特定的超响应和放大，门户容忍的机制并没有被清楚地理解。在这项研究中，我们调查了捐赠者细胞在宿主肝脏中的持久性和捐赠者特定混合淋巴细胞反应的抑制。The sequential changes in fluoresence labeled donor cells inoculated via the portal （PV组） or peripheral （IV组） vein were examined using fluoresence microscopy and flow cytometry. Fluorescent Positive Cells主要位于我的位置 ... More 第三天和第七天，对于Hepatic lobes的外围和中间区域，以及几个阳性细胞，每个Hepatic lobes，在第3天和第7天，PV组中的荧光阳性细胞数量明显大于第4组。更定量地，对孤立的半胱氨酸非半胱氨酸细胞的流动细胞分析揭示了荧光阳性细胞的百分比在PV组中明显高于第四组，但在第三天没有明显的差异可能不会在第七组之间看到明显的差异。MLR在第3天明显受到抑制，在第7天略微受到抑制，在第4组与较轻的MLR抑制相比，仅在第3天受到抑制。氯化钠（Gd）在门户暴露前2天内抑制了Kupffer细胞的噬细胞活性（GdPV组）的情况下，允许老鼠在2天内托管。在GdPV组捐赠者细胞持久性的高百分比和MLR对门户网站有效性的抑制在第四组每天3天损失较低的水平。口腔耐受性，是指在抗原特定时尚中对生物体生产和细胞免疫性的抑制作用，被认为是有可能应用于移植耐受性的。Donor spleen cell的内部管理（5×10 × D17 × D1/天× 5天），通过Jejunostomy tube自豪地抑制了Donor specific manager和foot pad反应，并显著地促进了Cardiac allograft survival。Gd管理局失去了这些捐赠者-特定的免疫学超应答和牙龈存活的延长。这些结果表明，宿主肝脏中持久性捐赠细胞的高百分比具有良好的核心作用，在捐赠者特定的MLR和Kupffer细胞中发挥了关键作用，并在门户耐受性中发挥了重要作用。Less（低）

The immunosuppressants used now a days in transplantation have not only incomplete inhibition of the rejection but also some incidense of complications such as oppotunistic infection, malignansies etc. hypo- or no response to the antigen is called immunological tolerance, which induces low or no incidense of rejection and results from the reduction of immunosuppressants. Although portal venous inoculation of donor antigen was reported to induce donor-specific hyporesponsiveness and prolongation of the grafts in animal models, the mechanism of the portal tolerance has not been clearly understood. In this study, we investigated the corelation between donor cell persistence in host liver and the suppression of donor-specific mixed lymphocyte reaction. The sequential changes in fluoresence labeled donor cells inoculated via the portal (PV group) or peripheral (IV group) vein were examined using fluoresence microscopy and flow cytometory. The fluorescent positive cells were mainly located i … More n the sinusoid of periportal and midzonal area of hepatic lobules, and several and a few positive cells per hepatic lobules on day 3 and 7, respectively. The number of fluoresent positive cells was seen impresively greater in PV group than in IV group on day 3 and 7. More quantitatively, flow cytometric analysis of the isolated hepatic non-parenchymal cells revealed that the percentage of fluoresent positive cells were significantly higher in PV group than in IV group on day 3, but no significant difference could not be observed between both groups on day 7. MLR was significantly suppressed on day 3 and slightly on day 7 in PV group, comparing with slight MLR suppression only on day 3 in IV group. Gadolinium chloride (Gd) which inhibits the phagocytic activity of Kupffer cell was given intravenously to host rats on 2 consecutive days before portal venous inoculation (GdPV group). In GdPV group high percentage of donor cell persistence and the suppression of MLR by portal venous inoculation were lost to the low level of IV group on day 3. Oral tolerance, which induces the suppression of anbibody production and cellular immunity in an antigen specific fashion, was studied to be able to apply to the transplantation tolerance. Intra-jejunal administration of donor spleen cells (5×10ィイD17ィエD1/day×5days) through the jejunostomy tube was proud to suppress the MLR and foot pad reaction in a donor specific manner, and significantly prolonge the cardiac allograft survival. Gd administration lost these donor-specific immunological hyporesponsiveness and the prolongation of gaft survival. These results indicates that high percentage of persistent donor cells in the host liver was good corelation with the suppression in donor-specific MLR and Kupffer cells play a crucial role in the portal tolerance. Less