Coordination Funds

协调基金

• 批准号: 437224664
• 负责人: Professor Dr. Jens Rolff
• 金额: --
• 依托单位: Arbeitsgruppe Rolff - Evolutionsbiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/437224664?language=en
• 关键词: Coordination; Funds

The overall goal of this research unit is to understand the impacts of the host immune system and the resident microbiota on pathogen virulence and resistance evolution. We study insects as they are excellent models, crucial providers of ecosystem services, important vectors of disease and the most diverse group of animals. To address our goal, we combine six empirical projects with one integrative theoretical and one bioinformatic project. We will use six insect species representing the three most speciose groups as host, and a suite of mostly bacteria, symbionts and pathogens. The new projects are based on a conceptual framework resulting from the current funding period. P1 will investigate the contributions of a host, the microbiota and a pathogen on the evolution of virulence using the fruit fly as a model. This will be achieved by decomposing virulence into host resistance and tolerance, and pathogen exploitation and per parasite pathogenicity and focus on non-competitive interactions. P2 is a theoretical project that will build a mathematical framework for the research unit to understand the consequences of tripartite interactions between hosts, microbiota and pathogens for pathogen virulence evolution. It will use the tools of population dynamics and models to understand interactions and collaborate with all other projects. P3 will use the co-evolved honey bee-Paenibacillus system to study within-host virulence evolution of a spore-forming necrotrophic pathogen. It will study the co-evolutionary interactions between host and pathogen mediated by the microbiota. In P4, using Lagria beetles and their symbiotic Burkholderia bacteria, the evolution of symbiont establishment in the light of defense cocktails will be studied providing a deeper insight into co-evolution in a tripartite interaction. P5 will focus on the host side: how is the regulation of AMPs in the host altered by the presence/absence of microbiota? Using the mealworm beetle the evolution of virulence in P. entomophila will be studied. In P6, using roaches and termites, the evolution of host immune genes in response to the presence/absence of symbiotic Blattabacteria will be investigated, allowing comparative insights into how symbiont co-evolution has shaped host immune defenses and its consequence for pathogen virulence. P7 will investigate the virulence -transmission tradeoff in a pathogen with spores. It will use red flour beetles as hosts and investigate virulence evolution in the presence or absence of the microbiota. The bioinformatic project including the technical support will be pivotal as most empirical projects will partially rely on next generation sequencing. A postdoc will lead this work. We argue that our combined approach is essential to gain new insight into host-microbiota-pathogen interactions and their consequences for the evolution of pathogen virulence.

这个研究单位的总体目标是了解宿主免疫系统和驻留微生物区系对病原菌毒力和抗性进化的影响。我们研究昆虫是因为它们是优秀的模型，是生态系统服务的关键提供者，是疾病的重要媒介，也是最多样化的动物群体。为了实现我们的目标，我们将六个经验项目与一个综合理论项目和一个生物信息学项目结合在一起。我们将使用代表三个最特殊类群的六种昆虫作为宿主，以及一套主要由细菌、共生体和病原体组成的寄主。新项目的基础是本供资期间产生的概念框架。P1将以果蝇为模型，研究寄主、微生物区系和病原体在毒力进化中的作用。这将通过将毒力分解为寄主抗性和耐受性、病原菌开发和每个寄生虫的致病性并关注非竞争性相互作用来实现。P2是一个理论项目，将为研究单位建立一个数学框架，以了解宿主、微生物区系和病原体之间的三方相互作用对病原体毒力进化的影响。它将使用种群动力学和模型的工具来了解相互作用并与所有其他项目合作。P3将使用共同进化的蜜蜂-拟青霉系统来研究一种形成孢子的坏死性病原体的寄主内毒力进化。它将研究微生物区系介导的寄主和病原体之间的共同进化相互作用。在P4中，使用拉格里亚甲虫和它们的共生伯克霍尔德氏菌，将研究在防御鸡尾酒的情况下共生体的建立的进化，提供对三方相互作用中的共同进化的更深层次的洞察。P5将重点放在宿主一侧：宿主中AMP的调节如何因微生物区系的存在/不存在而改变？利用粉虫甲虫，将研究嗜食昆虫病原虫毒力的进化。在P6中，使用蟑螂和白蚁，将研究宿主免疫基因的进化，以响应共生Blattabacteria的存在/不存在，从而能够比较深入地了解共生菌的共同进化如何塑造宿主免疫防御及其对病原体毒力的影响。P7将研究病原体和孢子之间的毒力-传播权衡。它将使用红粉甲虫作为宿主，并在微生物区系存在或不存在的情况下研究毒力进化。生物信息学项目，包括技术支持，将是关键，因为大多数经验性项目将部分依赖于下一代测序。一位博士后将领导这项工作。我们认为，我们的组合方法对于获得对宿主-微生物群-病原体相互作用及其对病原体毒力进化的影响的新见解至关重要。