Elucidation of the structure of angiotensin II receptor and its application to drug design

对血管紧张素II （AII）和心房钠素肽（ANP）受体进行了纯化和表征。从牛肾上腺皮质膜中溶解AII受体，通过离子交换层析、疏水层析和凝集素亲和层析进行部分纯化。纯化后的制剂经辐射失活、亲和标记和化学修饰表征。对配体结合位点的结构也进行了探讨。从牛肺质膜中完全纯化ANP受体。电泳和n端分析表明ANP受体由两个相同的二硫连接的70-KDa亚基组成。对纯化的ANP受体产生抗血清，并成功地用于受体的免疫组织定位和受体cDNA的克隆。

Receptors for angiotensin II (AII) and atrial natriuretic peptide (ANP) were purified and characterized. AII receptors were solubilized from bovine adrenal cortex membranes and partially purified by ion exchange chromatography, hydrophobic chromatography and lectin afinity chromatoraphy. The purified preparation was characterized by radiation inactivation, affinity labeling, and chemical modification. The sturcture of the ligand-binding site was also probed.ANP receptors were completely purified from bovine lung plasma membranes. Electrophoretic and N-terminal analysisi indicated that the ANP receptor is composed of two identical disulfide-linked 70-KDa subunits. Antiserum was raised apainst the purified ANP receptor and used successfully in immunohistoceical localiation of the recepror and in cloning of receptor cDNA.