Elucidation of the structure of angiotensin II receptor and its application to drug design

血管紧张素II受体结构的阐明及其在药物设计中的应用

• 批准号: 60480467
• 负责人: HIROSE Shigehisa
• 金额: $ 4.29万
• 依托单位: Tokyo Institute of Technology (1986-1987)University of Tsukuba (1985)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480467/
• 关键词: Angiotensin II; receptor; Atrial natriuretec peptide; Blld pressure; Auti-receptor antibody; 免疫組織化学; レセプター; 心房性ナトリウケ利尿ホルモン; 内皮細胞; レセプター抗体; Radiation lnactivation; アンジオテンシン【II】; 心房性ナトリウム利尿ホルモン

Receptors for angiotensin II (AII) and atrial natriuretic peptide (ANP) were purified and characterized. AII receptors were solubilized from bovine adrenal cortex membranes and partially purified by ion exchange chromatography, hydrophobic chromatography and lectin afinity chromatoraphy. The purified preparation was characterized by radiation inactivation, affinity labeling, and chemical modification. The sturcture of the ligand-binding site was also probed.ANP receptors were completely purified from bovine lung plasma membranes. Electrophoretic and N-terminal analysisi indicated that the ANP receptor is composed of two identical disulfide-linked 70-KDa subunits. Antiserum was raised apainst the purified ANP receptor and used successfully in immunohistoceical localiation of the recepror and in cloning of receptor cDNA.

对血管紧张素II （AII）和心房钠素肽（ANP）受体进行了纯化和表征。从牛肾上腺皮质膜中溶解AII受体，通过离子交换层析、疏水层析和凝集素亲和层析进行部分纯化。纯化后的制剂经辐射失活、亲和标记和化学修饰表征。对配体结合位点的结构也进行了探讨。从牛肺质膜中完全纯化ANP受体。电泳和n端分析表明ANP受体由两个相同的二硫连接的70-KDa亚基组成。对纯化的ANP受体产生抗血清，并成功地用于受体的免疫组织定位和受体cDNA的克隆。

项目成果

M. Shinjo: "Characteriation of atrial natriuretic factor receptors in adrenal cortex, vascular smooth muscle and exdothelial cells by affinity labeling." Biomedical Res.7. 35-38 (1986)

M. Shinjo：“通过亲和标记来表征肾上腺皮质、血管平滑肌和外皮细胞中的心房钠尿因子受体。”

M. Shinjo: Biomedical Res.7. 35-38 (1986)

M. Shinjo：生物医学研究7。

H.Shionoiri: Biochem.Biophys.Res.Commun.148. 286-291 (1987)

H.Shionoiri：Biochem.Biophys.Res.Commun.148。

M.Ishido: Biochem.Biophys.Res.Commun.140. 101-106 (1986)

M.Ishido：Biochem.Biophys.Res.Commun.140。

S. Hirose: Biochem. Biophys. Res. Commun.130. 574-579 (1985)

S. Hirose：生物化学。