Visualization and characterization of GM2-rich lipid raft microdomain

富含 GM2 的脂筏微域的可视化和表征

• 批准号: 22657029
• 负责人: HIROSE Shigehisa
• 金额: $ 2.15万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657029/
• 关键词: 脂質; タンパク質; 糖鎖; 細胞膜; 脂質ラフト; ガングリオシド; GOTO細胞; モノクローナル抗体; フロチリン; エンドサイトーシス; 神経芽腫; シクロデキストリン; GM2

We raised a monoclonal antibody which enables the visualization of ganglioside GM2-rich microdomains as highly punctate dots on a human neuroblastoma cell, GOTO. Using this antibody, we showed that raft-like microdomains of GM2(GM2rafts) were colocalized with flotillin-2/reggie-1-positive microdomains. Prelabeling of GM2 rafts with a fluorescent probe, Alexa555. Anti-GM2, demonstrated internalization and delivery of the raft. flotillin assembly to the recycling endosomes. Depletion of cholesterol with methyl-β-cyclodextrin(MβCD) resulted in disruption and targeting of GM2 rafts to lysosomes. These results suggest that in GOTO cells, GM2 rafts are actually present in association with flotillin and undergo internalization into distinct pools of endosomal vesicles depending on their cholesterol contents.

我们提出了一种单克隆抗体，使可视化的神经节苷脂GM 2丰富的微域作为高度点状点的人神经母细胞瘤细胞，后藤。使用这种抗体，我们发现GM 2的筏状微结构域（GM 2 rafts）与flotillin-2/reggie-1阳性微结构域共定位。用荧光探针Alexa 555预标记GM 2筏。抗GM 2，表现出筏的内在化和传递。flotillin组装到再循环内体。用甲基-β-环糊精（MβCD）去除胆固醇导致GM 2筏被破坏并靶向溶酶体。这些结果表明，在后藤细胞中，GM 2筏实际上存在于与flotillin和经历内化到不同的池的内体囊泡取决于其胆固醇含量。

项目成果

Methyl-beta-cyclodextrin-mediatedlipid raft disruption causes acuteapoptosis in neuroblastoma GOTO cell

甲基-β-环糊精介导的脂筏破坏导致神经母细胞瘤GOTO细胞急性凋亡

• 发表时间: 2010
• 作者: Tomioka;R.;Kato;A.;and Hirose;S
• 通讯作者: S

Differential expression of Na+-Cl- cotransporter and Na+-K+-Cl- cotransporter 2 in the distal nephrons of euryhaline and seawater pufferfishes

• DOI: 10.1152/ajpregu.00725.2009
• 发表时间: 2011-02-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
• 影响因子: 2.8
• 作者: Kato, Akira;Muro, Takayuki;Hirose, Shigehisa
• 通讯作者: Hirose, Shigehisa

Identification of zebrafish Fxyd11a protein that is highly expressed in ion-transporting epithelium of the gill and skin and its possible role in ion homeostasis

• DOI: 10.3389/fphys.2010.00129
• 发表时间: 2010-01-01
• 期刊: FRONTIERS IN PHYSIOLOGY
• 影响因子: 4
• 作者: Saito, Kaori;Nakamura, Nobuhiro;Hirose, Shigehisa
• 通讯作者: Hirose, Shigehisa

From blood typing to a transport metabolon

从血型到运输代谢

• 发表时间: 2010
• 期刊: Am.J.Physiol.Cell.Physiol.
• 作者: Hirose;S.;Nakada;T.
• 通讯作者: T.

ガングリオシドGM2を主成分とするラフトの挙動

以神经节苷脂GM2为主要成分的筏的行为

• 发表时间: 2012
• 作者: Yabuki A;Nakayama T;Yubuki N;Hashimoto T;Ishida K;Inagaki Y;広瀬茂久
• 通讯作者: 広瀬茂久