Regulation of Transcription by cAMP and cAMP receptor protein in Escherichia coli

大肠杆菌中 cAMP 和 cAMP 受体蛋白的转录调控

• 批准号: 60480511
• 负责人: AIBA Hiroji
• 金额: $ 4.29万
• 依托单位: University of Tsukuba (1986-1987)Kyoto University (1985)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480511/
• 关键词: cAMP; cAMp receptor protein; promoter; regulation of transcription; CRPレギュロン; プロモーター

The transcription of Escherichia coli cya gene by cAMP and its receptor protein(CRP) has been studied. The transcription of cya gene was shown to be negatively regulated by cAMP-CRP in vitro. The cAMP-CRP complex binds specifically to the cya promoter region to inhibit the transcription. The negative regulation of the cya transcription was also shown in vivo by S1 nuclease and RNA dot blot assays. The cya-lac gene fusion studies gave another evidence for the cya regulation by cAMP- CRP.The whole genome of E.coli was digested by a restriction enzyme and the resulting DNA fragments were randomly cloned into a promoter selection cloning vector. Among about 100 clones, 5 have been shown to contain promoters that can be activated by cAMP. Additionally one promoter, which transcription is represses by cAMP, was also isolated. The DNA sequences of these promoters have been determined. Most of promoters sequenced turned out to corresponds to unknown genes.

研究了cAMP及其受体蛋白(CRP)对大肠杆菌cya基因的转录作用。体外表明cya基因的转录受到cAMP-CRP的负调控。 cAMP-CRP 复合物特异性结合 cya 启动子区域以抑制转录。 S1 核酸酶和 RNA 斑点印迹测定也显示了体内 cya 转录的负调控。 cya-lac基因融合研究为cAMP-CRP调节cya提供了另一个证据。用限制性酶消化大肠杆菌的整个基因组，并将所得DNA片段随机克隆到启动子选择克隆载体中。在大约 100 个克隆中，有 5 个已显示含有可被 cAMP 激活的启动子。另外，还分离出一种启动子，其转录被cAMP抑制。这些启动子的DNA序列已经确定。大多数经测序的启动子都对应于未知基因。

项目成果

K. Mori & H. Aiba: "Evidence for negative control of cya transcription by cAMP and cAMP receptor protein in intact Escherichia coli cells" J. Biol. Chem.260. 14838-14843 (1985)

森 K.

H. Aiba;T. Nakamura;h. Mitani & H. Mori: EMBO J.4. 3329-3332 (1985)

H.相叶；T.

H. Aiba: Adv. Biophys. 21. 193-204 (1986)

H.相叶：Adv。

H. Aiba: "Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein." J. Biol. Chem.260. 3063-3070 (1985)

H. Aiba：“大肠杆菌腺苷酸环化酶基因的转录受到 cAMP-cAMP 受体蛋白的负调控。”

H. Aiba: "Negative control of transcription by cAMP and cAMP receptor protein in Escherichia coli" Adv. Biophys.21. 193-204 (1986)

H. Aiba：“大肠杆菌中 cAMP 和 cAMP 受体蛋白对转录的负控制”Adv。