Mechanism of tmRNA-mediated trans-translation

tmRNA介导的反式翻译机制

• 批准号: 14035201
• 负责人: AIBA Hiroji
• 金额: $ 43.2万
• 依托单位: Nagoya University (2005-2006)Hirosaki University (2002-2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14035201/
• 关键词: tmRNA; trans-translation; mRNA cleavage; nonstop mRNAs; ribosome; translation surveillance; ribosome stalling; rare codon; 翻訳サーベイランス; レアコドン; mRNA decay; 大腸菌; 枯草菌; 抗生物質; タンパク質品質管理; mRNA; パロモマイシン; tRNA; タンパク質分解

1) Ribosome stalling by the rare codon induces mRNA cleavage, resulting in nonstop mRNAs that are recognized by tmRNA.2) Ribosome stalling at the cognate stop codon caused by reduced activity or expression of RF-1 or RF-2 induces mRNA cleavage, resulting in nonstop mRNAs that are recognized by tmRNA.3) Ribosome stalling caused by amino acid starvation induces mRNA cleavage at the corresponding codons, resulting in nonstop mRNAs that are recognized by tmRNA.4)The ribosome-mediated mRNA cleavage along with the tmRNA system is an important mechanism that enables cells to adapt to amino acid starvation conditions.5) Trans-translation occurs at the A-site of the ribosome without cleavage of mRNA, and that compeptition among tRNA, tmRNARF and RRF occurs at the A-site.6) SmpB binds not only the tRNA domain but also the mRNA domain of tmRNA to determine the initiation point of tag-translation.7) In vitro trans-translation system was developed using purified translation factors. Using this system, we revealed that trans-translation does not require S 1 protein.8) We have proposed new concepts, "a protein mimicking tRNA+mRNA" and "a protein encoding an amino acid".

1)由稀有密码子引起的核糖体停滞诱导mRNA切割，导致被tmRNA识别的不停止的mRNA。2）由RF-1或RF-2的活性或表达降低引起的核糖体在同源终止密码子处停滞诱导mRNA切割，导致被tmRNA识别的不停止的mRNA。3）由氨基酸饥饿引起的核糖体停滞诱导mRNA在相应密码子处切割，4）核糖体介导的mRNA切割沿着tmRNA系统是使细胞适应氨基酸饥饿条件的重要机制。5）反式翻译发生在核糖体的A位点而不切割mRNA，并且tRNA之间的竞争，6）SmpB不仅与tmRNA的tRNA结构域结合，还与tmRNA的mRNA结构域结合，决定tag-translation的起始点。7）利用纯化的翻译因子建立了体外反式翻译系统。8）我们提出了“一种模拟tRNA+mRNA的蛋白质”和“一种编码氨基酸的蛋白质”的新概念。

项目成果

Reduced action of polypeptide release factors induces mRNA cleavage and tmRNA tagging at stop codons in Escherichia coli

• DOI: 10.1111/j.1365-2958.2006.05498.x
• 发表时间: 2007-01-01
• 期刊: MOLECULAR MICROBIOLOGY
• 影响因子: 3.6
• 作者: Li, Xia;Yokota, Teruaki;Aiba, Hiroji
• 通讯作者: Aiba, Hiroji

トランストランスレーション

恍惚翻译

• 发表时间: 2008
• 期刊: キーワード : 蛋白質の一生(蛋白質核酸酵素増刊) 53
• 作者: 須山研吾;五味勝也;新谷尚弘;姫野俵太
• 通讯作者: 姫野俵太

タンパク質科学イラストレイテッド

蛋白质科学画报

• 发表时间: 2005
• 作者: Chan;W.;Nishikimi;A.;Kamata;T.;Adachi;Y.;竹縄忠臣 編集
• 通讯作者: 竹縄忠臣 編集

Interaction analysis between tmRNA and SmpB from Thermus thermophilus.

• DOI: 10.1093/jb/mvi180
• 发表时间: 2005-12
• 期刊: Journal of biochemistry
• 影响因子: 2.7
• 作者: N. Nameki;T. Someya;S. Okano;Reiko Suemasa;M. Kimoto;K. Hanawa-Suetsugu;T. Terada;M. Shirouzu;I. Hirao;H. Takaku;H. Himeno;A. Muto;S. Kuramitsu;S. Yokoyama;G. Kawai

Implication of membrane localization of target mRNA in the action of a small RNA:: mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli

• DOI: 10.1101/gad.1270605
• 发表时间: 2005-02-01
• 期刊: GENES & DEVELOPMENT
• 影响因子: 10.5
• 作者: Kawamoto, H;Morita, T;Aiba, H
• 通讯作者: Aiba, H