Molecular basis of the action of Hfq-binding small RNA inEscherichia coli.

以SgrS为模型，研究了hfq结合sRNA功能的分子基础。主要研究结果如下：(1) SgrS中的14nt区域是ptg mRNA碱基配对和翻译抑制所需的最小区域。(2) RNase E的711 ~ 750区域足以与Hfq进行功能相互作用，支持ptsG mRNA的快速降解。(3) rho无关终止子的polyU尾对Hfq的作用至关重要。(4)细菌sRNAs的功能性hfq结合模块由双或单发夹组成，发夹前面是一个富U序列，后面是一个3 ' poly(U) tail。(5)成功设计了与hfq结合的小rna敲除所需mrna。

Molecular basis of the function of Hfq-binding sRNA has been studied using SgrS as a model. The major findings are as follows. (1) The 14 nt region within SgrS is the minimum region required for base-pairing and translational repression of ptsG mRNA. (2) The region between 711 and 750 of RNase E is sufficient for the functional interaction with Hfq to support the rapid degradation of ptsG mRNA. (3) The polyU tail of rho-independent terminator is essential for Hfq action. (4) The functional Hfq-binding module of bacterial sRNAs consists of a double or single hairpin preceded by a U-rich sequence and followed by a 3’ poly(U) tail. (5) The synthetic Hfq-binding small RNAs to knock down desired mRNAs have been successfully designed.