Molecular basis of the action of Hfq-binding small RNA inEscherichia coli.

使用 SgrS 作为模型研究了 Hfq 结合 sRNA 功能的分子基础。主要调查结果如下。 (1) SgrS 内的 14 nt 区域是 ptsG mRNA 碱基配对和翻译抑制所需的最小区域。 (2) RNase E 711和750之间的区域足以与Hfq发生功能性相互作用，以支持ptsG mRNA的快速降解。 (3) rho 独立终止子的 PolyU 尾对于 Hfq 作用至关重要。 (4) 细菌 sRNA 的功能性 Hfq 结合模块由双发夹或单发夹组成，前面是富含 U 的序列，后面是 3' 聚 (U) 尾。 (5) 成功设计了合成的Hfq结合小RNA来敲低所需的mRNA。

Molecular basis of the function of Hfq-binding sRNA has been studied using SgrS as a model. The major findings are as follows. (1) The 14 nt region within SgrS is the minimum region required for base-pairing and translational repression of ptsG mRNA. (2) The region between 711 and 750 of RNase E is sufficient for the functional interaction with Hfq to support the rapid degradation of ptsG mRNA. (3) The polyU tail of rho-independent terminator is essential for Hfq action. (4) The functional Hfq-binding module of bacterial sRNAs consists of a double or single hairpin preceded by a U-rich sequence and followed by a 3’ poly(U) tail. (5) The synthetic Hfq-binding small RNAs to knock down desired mRNAs have been successfully designed.